Apheresis platelets were obtained from the Blood Center of Jiangsu Province China. L‐arginine was obtained from Sigma–Aldrich (St. Louis, MO, USA). Fingolimod (FTY720) hydrochloride was purchased from Selleck (Houston, TX, USA). H9C2 cells, THP‐1 cells, and HUVECs were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). RPMI 1640 and Dulbecco's Modified Eagle's medium (DMEM) were obtained from KeyGEN Biotech (China). DiO (excitation/emission wavelength of 483/ 501, green color), DiR (excitation/emission wavelength of 754/ 778), and DiI (excitation/emission wavelength of 551/569, red color) were acquired from Beyotime (Haimen, China).
Dulbecco s modified eagle s medium dmem
Manufactured by Keygen Biotech
Sourced in China
Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium commonly used to support the growth of various cell types in vitro. It is a modification of the original Eagle's minimal essential medium, designed to provide a balanced salt solution and additional nutrients required for cell proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Keygen Biotech (5 mentions)
Streptomycin, by Keygen Biotech (3 mentions)
Penicillin, by Keygen Biotech (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Lysotracker red, by Keygen Biotech (2 mentions)
Huvecs, by National Collection of Authenticated Cell Cultures (1 mentions)
Thp 1 cells, by National Collection of Authenticated Cell Cultures (1 mentions)
L arginine, by Merck Group (1 mentions)
Rpmi 1640, by Keygen Biotech (1 mentions)
Manganese chloride tetrahydrate, by Merck Group (1 mentions)
Bovine serum protein bsa, by Merck Group (1 mentions)
Fitc labeled goat anti mouse igg, by Beyotime (1 mentions)
293t cells, by National Collection of Authenticated Cell Cultures (1 mentions)
Skov3, by Keygen Biotech (1 mentions)
Mccoy s 5a medium, by Keygen Biotech (1 mentions)
A2780, by Keygen Biotech (1 mentions)
Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions)
20 protocols using dulbecco s modified eagle s medium dmem
1
Apheresis Platelet Isolation and Characterization
2
Reactive Oxygen Species Sensing Nanoplatform
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Manganese chloride tetrahydrate (MnCl2•4H2O), tetramethylammonium hydroxide solution (TMA, 1.0 M), bovine serum protein (BSA), and 2′,7′-dichloroflourescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich. NH2-PEG3000 and chlorin e6 (Ce6) were purchased from JenKem Technology and Frontier, respectively. The hydrogen peroxide (H2O2, 30 wt.%) solution was purchased from Sinopharm Chemical Reagent Co., Ltd. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were bought from KeyGen BioTech and Gibco, respectively. HIF-1α antibody (mouse monoclonal antibody) and FITC-labeled goat anti-mouse IgG were purchased from Beyotime Biotechnology. All solutions were prepared by using ultrapure water (18.2 MΩ).
3
Cell Line Procurement and Cultivation
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The ovarian cancer cell lines SKOV3, A2780, and HO8910 were purchased from KeyGEN Biotech (Nanjing, China); 293T cells were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China); immortalized ovarian surface epithelial cells 80 (IOSE80) were a gift from Dr Li Jing in Nanjing Medical University. All cell lines were verified by STR profiling. SKOV3 cells were cultured in McCoy’s 5A medium (KeyGEN Biotech) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, USA), 100 ng/mL streptomycin, and 100 U/mL penicillin (Thermo Fisher Scientific). A2780, HO8910, and 293T cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; KeyGEN Biotech) supplemented with 10% FBS, 100 ng/mL streptomycin, and 100 U/mL penicillin. IOSE80 cells were cultured in RPMI-1640 supplemented with 10% FBS, 100 ng/mL streptomycin, and 100 U/mL penicillin. All cells were cultured at 37°C in an incubator with a humidified atmosphere containing 5% CO
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4
Seed Meal Transglutaminase Hydrogel for Cancer
5
Extraction and Evaluation of Cyperus rotundus L. Bioactivity
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The dry rhizomes of Cyperus rotundus L. were purchased from Anhui Xiehecheng Co., Ltd. (Bozhou, China); the ethanol extract of Cyperus rotundus L. was acquired referring to previous reporting method [10 ]; Dulbecco’s modified Eagle’s medium (DMEM), penicillin, and streptomycin were purchased from Nanjing KeyGen Biotech. Co. Ltd. (Jiangsu, China); Fetal bovine serum (FBS) and Phosphate-buffered saline (PBS) was purchased from Gibco Company (New York, U.S.A.); Dimethyl sulfoxide (DMSO) was purchased from Thermo Fisher Scientific (Massachusetts, U.S.A.); The Cell Counting Kit-8 assay (CCK-8) was purchased from MedChemExpress (New Jersey, U.S.A.); Acetonitrile (liquid chromatogram grade) was purchased from Merck KGaA (Darmstadt, Germany); Formic acid was purchased from Honeywell Trading Co., Ltd. (Muskegon, USA).
6
Cell Culture Conditions for GBM
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Human GBM cell lines (U118, U87, U251, T98G, and LN229), human normal brain glial cells (HEB), and normal human astrocyte (NHA) were originally obtained from the American Type Culture Collection (ATCC). All these cell lines were cultured with Dulbecco’s modified Eagle’s medium (DMEM; KeyGen Biotech, China) containing 10% fetal bovine serum (FBS, Takara, Japan) at 37°C within a humid atmosphere containing 5% CO2.
7
Aconitine-Induced Cytotoxicity Assay
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Aconitine was obtained from the Chengdu Research Institute of Biology of the Chinese Academy of Sciences. MTT was obtained from Roche Diagnostics. Protein Extraction kit (cat. no. KGP250), penicillin and streptomycin combination were purchased from Nanjing KeyGen Biotech Co., Ltd. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS; cat. no. 10099-141) were purchased from Gibco; Thermo Fisher Scientific, Inc. 4′,6′-Diamidino-2-phenylindole (DAPI) was purchased from Sigma-Aldrich; Merck KGaA. Comet assay kit (cat. no. 4250) was purchased from Cell Biolabs, Inc. The TUNEL assay kit (cat. no. FA201) was purchased from Beijing Transgen Biotech Co., Ltd. JC-1 mitochondrial membrane potential assay kits (cat. no. C2006) were obtained from Beyotime Institute of Biotechnology. Primary and secondary antibodies were purchased from ProteinTech Group or BIOSS and are presented in Table I . BSA blocking buffer was purchased from Beijing Solarbio Science & Technology Co, Ltd (cat. no. SW3015).
8
Liposome-based Drug Delivery System
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LA was procured from Chengdu Luokema Biological Technology Co. (Chengdu, China). Cholesterol and soya lecithin were obtained from Shanghai Mecklin Biological Technology Co. (Shanghai, China). Polyethylene glycogen I 400 (PEG400) was obtained from Beijing Solabor Biological Technology Co. (Beijing, China). Lyso-Tracker Red, ceramide, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI), Cell Counting Kit-8 (CCK-8), trypsin, fetal bovine serum (FBS), and Dulbecco’s modified Eagle’s medium (DMEM) were bought from KeyGEN Bio TECH (Jiangsu, China). Sigma-Aldrich (St. Louis, MO, USA) supplied Coumarin-6. All other reagents used in this experiment were of analytical quality.
The Southern Medical University provided male Sprague–Dawley (SD) rats (200 ± 10 g). All animal experiments performed conformed to the animal handling guidelines established by the “Guiding Principles in the Care and Use of Animals”, and the procedures were approved by the Ethics Committee of Southern Medical University (L2019036, date of approval: 13 April 2019).
The Southern Medical University provided male Sprague–Dawley (SD) rats (200 ± 10 g). All animal experiments performed conformed to the animal handling guidelines established by the “Guiding Principles in the Care and Use of Animals”, and the procedures were approved by the Ethics Committee of Southern Medical University (L2019036, date of approval: 13 April 2019).
9
Triptolide-Loaded Lipid Nanoparticles
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Triptolide (TPL) was purchased from Shanghai Yuanye Biological technology co., (Shanghai, China). Compritol 888 ATO (solid lipid), Capryol 90 (liquid lipid), and Transcutol® HP was obtained from Gattefosse (Saint-Priest, France). 4% paraformaldehyde solution was purchased from Wuhan Google biological technology Co., Ltd. (Shanghai, China). Soya lecithin was obtained from Shanghai Taiwei Pharmaceutical Co., Ltd. (Shanghai, China). Coumarin-6 was provided by Sigma-Aldrich (St. Louis, MO). Tween 80 was purchased from Tianjin Guangcheng Chemical Agent Co., Ltd. (Tianjin, China). Enzyme linked immunosorbent assay (ELISA) were obtained from Bender Medsystems GmbH (South San Francisco, USA); Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), Lyso-Tracker Red, DAPI, Cell Counting kit-8 (CCK-8), Trypsin were obtained from KeyGEN Bio TECH (Jiangsu, China). The other chemicals and solvents were of analytical reagent grade.
Male SD rats (200 ± 10 g) and Wistar rats (300 ± 20 g) were purchased from the Second Military Medical University. And the animal experiments coincided with the protocols evaluated by the ethics committee of the Second Military Medical University.
Male SD rats (200 ± 10 g) and Wistar rats (300 ± 20 g) were purchased from the Second Military Medical University. And the animal experiments coincided with the protocols evaluated by the ethics committee of the Second Military Medical University.
10
Microglia and Astrocytoma Cell Co-culture
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N9 murine microglia and C6 rat astrocytoma cells were obtained from Bluef Biotechnology (Shanghai, China). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; KeyGEN BioTECH, China) supplemented with 10% heat-inactivated fetal bovine serum at 37 ℃ in a humidified incubator (5% CO2). N9 or C6 cells were incubated with 50 µL of platelets (1.60 × 106/mL) isolated from rats subjected to SAD or sham rats, clopidogrel (30 µM)- or vehicle (PBS)-pretreated platelets, or CD40L neutralizing antibody (30 µg/mL)- or vehicle (PBS)-pretreated platelets for 24 h.
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