1 palmitoyl 2 linoleoyl sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine is a lipid molecule that can be used as a building block for the synthesis of more complex lipids and biomembranes. It is a glycerophosphocholine with a palmitic acid and a linoleic acid attached to the glycerol backbone.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (2 citations)

11 protocols using 1 palmitoyl 2 linoleoyl sn glycero 3 phosphocholine

Lipid Bilayer Preparation and Functionalization

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLinPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1-palmitoyl-2-(9′-oxo-nonanoyl)-sn-glycero-3-phosphocholine (POxnoPC), cholesterol, 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (Biotin-DPPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhodamine-DPPE) were purchased from Avanti Polar Lipids. Avidin and succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)Hexanoate (NBD NHS-ester) were obtained from Invitrogen. Amino-dPEG12-alcohol was purchased from Quanta Biodesign. Poly(dimethylsiloxane) (PDMS) was purchased from Dow Chemical, and the indium-tin-oxide (ITO) coated glass was obtained from Delta Technologies. α-tocopherol and all other chemicals were purchased from Sigma-Aldrich.

Runas K.A, & Malmstadt N. (2015). Low levels of lipid oxidation radically increase the passive permeability of lipid bilayers. Soft matter, 11(3), 499-505.

+ Open protocol

+ Expand

Radiolysis of Lipid Liposomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

HOCH 2 CH 2 SH and 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) were purchased from Aldrich. 1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and 1-stearoyl-2-arachidonoyl-snglycero-3-phosphocholine (SAPC) were purchased from Avanti Polar Lipids (Alabaster, AL) (see Fig 1B). All solvents were HPLC-grade and the utilized water was glass-distilled. Radiolysis was performed at room temperature (22 ± 2 °C) on 1 mL samples using a 60 Co Gammacell. For the construction of 100 nm liposomes the Mini Extruder was used from Avanti Polar Lipids. The composition of lipids during the experiments was monitored by gas chromatography using the GC 7890Β from Agilent equipped with a flame ionization detector. The column used was the DB-23 capillary column (60m × 0.25mm). The heating was carried out at a temperature of 165 °C for 3 minutes followed by an increase of 1 o C/min up to 195 °C. The temperature remained at 195 °C for 40 minutes and then it was followed by an increase of 10 °C /min up to 240 °C for 10 minutes.

Tremi I., Anagnostopoulos D., Spyratou E., Gkeka P., Georgakilas A.G., Chatgilialoglu C, & Cournia Z. (2018). Effect of 5-trans Isomer of Arachidonic Acid on Model Liposomal Membranes Studied by a Combined Simulation and Experimental Approach. The Journal of membrane biology, 251(3).

+ Open protocol

+ Expand

Lipidomic Analysis of Bile Acids and Phospholipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

PPIX was purchased from Sigma-Aldrich (St. Louis, MO). Taurocholic acid (TCA), tauro-α-muricholic acid (T-α-MCA), tauro-β-muricholic acid (T-β-MCA), and β-muricholic acid (β-MCA) were obtained from Steraloids, Inc. (Newport, RI). 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PC 34:2) was purchased from Avanti Polar Lipids (Avanti Polar Lipids Inc., Alabaster, AL). N-palmitoyl-D-erythro-sphingosine (C16:0 Cer), N-stearoyl-D-erythro-sphingosine (C18:0 Cer), and N-nervonoyl-D-erythro-sphingosine (C24:1 Cer) were purchased from Cayman Chemical Company (Ann Arbor, MI). All solvents for ultra-performance liquid chromatography and time-of-flight mass spectrometry (UPLC-TOFMS) analysis were of the highest grade commercially available.

Wang P., Sachar M., Guo G.L., Shehu A.I., Lu J., Zhong X.B, & Ma X. (2018). Liver Metabolomics in a Mouse Model of Erythropoietic Protoporphyria. Biochemical pharmacology, 154, 474-481.

+ Open protocol

+ Expand

Lipid Standards for Metabolomics Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Linoleic acid (FA 18:2), cholesterol, butylhydroxytoluene (BHT), and 2-methoxypropene (MxP) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Cholesteryl linoleate (CE 18:2) was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PC 16:0/18:2) and 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC 16:0/0:0) were from Avanti Polar Lipids (Alabaster, AL, USA). All other reagents were of the highest grade available.

Kato S., Osuka Y., Khalifa S., Obama T., Itabe H, & Nakagawa K. (2021). Investigation of Lipoproteins Oxidation Mechanisms by the Analysis of Lipid Hydroperoxide Isomers. Antioxidants, 10(10), 1598.

+ Open protocol

+ Expand

Lipid and Oxylipin Standards for Lipidomic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PC 16:0/18:2), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (PC 16:0/18:1), and SPLASH® LIPIDOMIX® were purchased from Avanti Polar Lipids (Avanti Polar Lipids, Inc., Alabama, USA). 1,2-Dipalmitate-3-linoleate-glycerol (TG 16:0/16:0/18:2) and cholesteryl linoleate (CE 18:2) were purchased from Larodan (Solna, Sweden). Oxylipin standards (15(S)-hydroperoxyeicosatetraenoic acid, 15(S)-hydroxyeicosatetraenoic acid, 14(15)-epoxyeicosatetraenoic acid, 15-oxoeicosatetraenoic acid, 8(S),15(S)-dihydroxyeicosatetraenoic acid) were from Cayman Chemical (Ann Arbor, USA).

Criscuolo A., Nepachalovich P., Garcia-del Rio D.F., Lange M., Ni Z., Baroni M., Cruciani G., Goracci L., Blüher M, & Fedorova M. (2022). Analytical and computational workflow for in-depth analysis of oxidized complex lipids in blood plasma. Nature Communications, 13, 6547.

+ Open protocol

+ Expand

Phosphatidylcholine: Sourcing and Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phosphatidylcholine type S100 (soybean lecithin, phosphatidylcholine content ≥94%) and S-PC-3 (soybean lecithin, hydrogenated, phosphatidylcholine content ≥98%) were kindly provided by Lipoid GmbH, Ludwigshafen, Germany.
According to the manufacturer’s specification the fatty acids of the two acyl groups of phosphatidylcholine within the used lecithin types are listed in Table 1.
Phosphatidylcholine standards of 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (18:2–18:2 PC, DLPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (16:0–18:2 PC, PLPC), 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (16:0−18:0 PC, PSPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (16:0–16:0 PC, DPPC) were purchased from Avanti Polar Lipids (Alabama, US). Phosphatidylcholine standards 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (16:0–18:1 PC, POPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (18:1–18:1 PC, DOPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (18:0–18:0 PC, DSPC) were supplied from Lipoid GmbH (Ludwigshafen, Germany).

Zlomke C., Albrecht J, & Mäder K. (2020). Nicardipine Loaded Solid Phospholipid Extrudates for the Prevention of Cerebral Vasospasms: In Vitro Characterization. Pharmaceutics, 12(9), 817.

+ Open protocol

+ Expand

Lipid Standards for Lipidomics Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and n-oleoyl-d-erythro-sphingosylphosphorylcholine (SM(d18:1/18:1)) were obtained from Avanti Polar Lipids (AL, USA). Chloroform was purchased from Merck (Darmstadt, Germany). Water was purified (resistance > 18 mΩ/cm) on a PureLab Ultra Analytic System (ELGA Lab Water, Celle, Germany). Ammonium bicarbonate, ammonium formate, sodium ascorbate, and copper (II) sulfate anhydrous were purchased from Sigma-Aldrich (Munich, Germany). UPLC-grade methanol, acetonitrile, formic acid, and isopropanol were obtained from Biosolve VB (Valkenswaard, The Netherlands).

Coliva G., Lange M., Colombo S., Chervet J.P., Domingues M.R, & Fedorova M. (2020). Sphingomyelins Prevent Propagation of Lipid Peroxidation—LC-MS/MS Evaluation of Inhibition Mechanisms. Molecules, 25(8), 1925.

+ Open protocol

+ Expand

Phospholipid Profiling for Biological Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phospholipids (with purity of > 99%) including N-palmitoyl-D-erythro-sphingosylphosphorylcholine (SM(34:1)), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (PC(34:1)), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PC(34:2)), 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (PC(36:4)) and 1,2-dielaidoyl-sn-glycero-3-phosphocholine (PC(36:2)) were purchased from Avanti Polar lipids (Alabaster, AL, USA). 1,2-diastearoyl-sn-glycero-3-phosphocholine (PC(36:0)) as internal standard was purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). HPLC-grade dichloromethane, methanol, and acetonitrile were from Fisher Scientific (Fair Lawn, NJ, USA). Ultrapure water was purified by a Milli-Q system (Millipore, MA, USA).

Guo Y., Ren J., Li X., Liu X., Liu N., Wang Y, & Li Z. (2017). Simultaneous Quantification of Serum Multi-Phospholipids as Potential Biomarkers for Differentiating Different Pathophysiological states of lung, stomach, intestine, and pancreas. Journal of Cancer, 8(12), 2191-2204.

+ Open protocol

+ Expand

Phospholipid and Sphingolipid Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and n-oleoyl-d-erythro sphingosylphosphorylcholine (SM (d18:1/18:1)) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Methyl-tert-buthylether (MTBE) and chloroform were purchased from Sigma Aldrich (Taufkirchen, Germany). Potassium ferrocyanide (K₄[Fe(CN)₆]), disodium hydrogen phosphate (Na₂HPO₄), and sodium dihydrogen phosphate (NaH₂PO₄) were obtained from Merck (Darmstadt, Germany). Sodium sulfate was purchased from VWR (Darmstadt, Germany). UPLC-grade methanol, acetonitrile, formic acid, and isopropanol were obtained from Biosolve BV (Valkenswaard, Netherlands). Hydrogen peroxide (30%) and 32% ammonia solutions are from Carl Roth (Karlsruhe, Germany). 50 mM buffer solution of (Na₂HPO₄/NaH₂PO₄) (pH = 7.4) was used in the experiments. All chemicals employed were used without further purification. In all solutions and experiments ultrapure water was used (resistivity: 18.2 MΩ.cm, conductivity: 0.055 μS/cm) prepared by Thermo Scientific GenPure pro water purification system (Niederelbert, Germany).

Ravandeh M., Coliva G., Kahlert H., Azinfar A., Helm C.A., Fedorova M, & Wende K. (2021). Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress. Biomolecules, 11(2), 276.

+ Open protocol

+ Expand

Lipid and Protein Reagents for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-Bis palmitoyl-sn-glycero-3-phosphocholine (DPPC), egg yolk phosphatidylcholine (PC), phosphatidylglycerol (PG), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC), phosphatidylserine (PS), and cholesterol (chol) were purchased from Avanti-Polar Lipids (Birmingham, AL). Extracellular-signal-regulated kinase (Erk2) was purchased from Upstate (Millipore, Billerica, MA, USA). Protein concentration was measured by Coomassie blue binding using bovine γ-globulin as the standard (Bio-Rad, Hercules, CA, USA). A mouse monoclonal antibody against human Prdx6 was purchased from Chemicon EMD Millipore (Billerica, MA, USA). Bromoenol lactone (BEL) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Reduced glutathione (GSH), oxidized glutathione (GSSG), MJ33 (1-hexadecyl- 3-trifluoroethylglycero -sn-2-phosphomethanol), tris (2-carboxyethyl) phosphine (TCEP), dithiothreitol (DTT), and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Zhou S., Dodia C., Feinstein S.I., Harper S., Forman H.J., Speicher D.W, & Fisher A.B. (2018). Oxidation of Peroxiredoxin 6 in the Presence of GSH Increases its Phospholipase A2 Activity at Cytoplasmic pH. Antioxidants, 8(1), 4.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!