U118mg

Manufactured by American Type Culture Collection

Sourced in United States, China, United Kingdom, Switzerland

The U118MG is a human glioblastoma cell line, derived from a male patient with a primary brain tumor. It is a commonly used model for in vitro studies of glioblastoma, one of the most aggressive forms of brain cancer.

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U118MG cells (5 citations)

109 protocols using u118mg

Glioblastoma Cell Lines and Primary Cultures

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A172, T98G, U118MG and U87MG cell lines were purchased from ATCC. A172 cells (ATCC® CRL-1620™) and U118MG cells (ATCC® HTB-15™) were managed in cultivation flasks in DMEM medium (LGC Standard); T98G cells (ATCC® CRL-1690™) and U87MG (ATCC® HTB-14™) were cultivated in EMEM medium (LGC Standard). Both media were supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific) and 1% penicillin/streptomycin (Life Technologies, Thermo Fisher Scientific).
GBM clinical samples were obtained from patients undergoing surgery at University Hospital Hradec Králové. The study was approved by Ethics Committee, University Hospital Hradec Králové (Reference No. 201709 S13P) and patients gave their written informed consent. Cells were isolated by mechanic dissociation as described previously^{26 (link)} and all experiments were performed in accordance with relevant guidelines and regulations. GBM primary cultures (GBM49, GBM50, GBM57, GBM71, GBM72, GBM73) were grown in cultivation flask in cultivation medium: RPMI 1640 medium (Sigma-Aldrich) supplemented with 15% fetal bovine serum (Gibco, Thermo Fisher Scientific), insulin (100 IU/ml, Eli Lilly) and transferrin (2 mg/ml, Sigma-Aldrich). Cells were grown in humified atmosphere with 5% CO2 at 37 °C and subcultured every 3 days.

Vítovcová B., Skarková V., Havelek R., Soukup J., Pande A., Caltová K, & Rudolf E. (2023). Flubendazole exhibits anti-glioblastoma effect by inhibiting STAT3 and promoting cell cycle arrest. Scientific Reports, 13, 5993.

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Characterization of Human Glioblastoma Cell Lines

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The four human glioblastoma tumor cell lines such as T98-G, LN-18, U-118 MG, and U-138 MG were obtained from the American Type Culture Collection (Rockville, MD) and were grown in Eagle’s Minimum Essential medium supplemented with 10% fetal bovine serum (U-138 MG, LN-18) or Dulbecco’s Modified Eagle medium with 10% fetal bovine serum (U-118 MG, T98-G). All glioma cell lines were kindly provided by Prof. Jan Barciszewski (NanoBioMedical Center, Adam Mickiewicz University in Poznan and Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland). The cells’ age did not exceed 15 passages.
The HeLaPKCεA/E subline was derived from parental HeLa wild-type cells, using transfection as previously described [21 (link)]. Created cell line shows constitutively active PKCε without activators such as TPA (12-O-Tetradecanoylphorbol-13-acetate). The HeLaPKCεA/E cells were grown in DMEM medium supplemented with 10% Tet-Approved fetal bovine serum (Clontech, Mountain View, USA), 100 μg/ml geneticin, and 100 μg/ml hygromycin (both from Roche Diagnostics, Mannheim, Germany). All cells were propagated in a humidified atmosphere containing 5% CO₂ at 37 °C.

Toton E., Romaniuk A., Konieczna N., Hofmann J., Barciszewski J, & Rybczynska M. (2018). Impact of PKCε downregulation on autophagy in glioblastoma cells. BMC Cancer, 18, 185.

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Culturing Human Malignant Glioma Cell Lines

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Human malignant glioma cell lines (U118MG, U251MG, SF126, SHG-44, U87MG) were obtained from ATCC. All cells were cultured in DMEM medium supplemented with 10% (v/v) fetal bovine serum (FBS) in a humidified incubator with 5% CO₂ at 37°C.

Guo J., Xue Q., Liu K., Ge W., Liu W., Wang J., Zhang M., Li Q.Y., Cai D., Shan C., Zhang C., Liu X, & Li J. (2019). Dimethylaminomicheliolide (DMAMCL) Suppresses the Proliferation of Glioblastoma Cells via Targeting Pyruvate Kinase 2 (PKM2) and Rewiring Aerobic Glycolysis. Frontiers in Oncology, 9, 993.

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Establishment and Validation of Glioma and Meningioma Cell Lines

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Two human glioma cell lines, U118-MG and U87-MG from ATCC (Manassas, VA), and IOMM and KT21 human meningioma cell lines were provided by Gregory Riggins (Johns Hopkins University). Three canine glioma cell lines, SDT-3g, GO6A, and J3T-Bg, were provided by Peter Dickinson (UC Davis). Cells were cultured at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with penicillin (100 IU/mL), streptomycin (100 IU/mL), and 10% fetal bovine serum with 5% CO₂. Cell cultures were maintained in subconfluent monolayers and passaged 2–3 times weekly as needed. Cell lines were tested by STR (short tandem repeat) analysis at the University of Arizona (human) and at the Flint Animal Cancer Center Cell Line Validation Core at Colorado State University (canine).

Schlein L.J., Fadl-Alla B., Pondenis H.C., Lezmi S., Eberhart C.G., LeBlanc A.K., Dickinson P.J., Hergenrother P.J, & Fan T.M. (2019). Immunohistochemical Characterization of Procaspase-3 Overexpression as a Druggable Target With PAC-1, a Procaspase-3 Activator, in Canine and Human Brain Cancers. Frontiers in Oncology, 9, 96.

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Establishing Primary Astrocyte and Glioma Cell Cultures

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Primary normal human astrocytes (NHAs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured according to the manufacturer’s instructions. Glioma cell lines A172, T98G, LN18, LN229, U138MG, U87, and U118MG were from ATCC (Manassas, VA, USA). The cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Wang H., Pan J.Q., Luo L., Ning X.J., Ye Z.P., Yu Z, & Li W.S. (2015). NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma. Molecular Cancer, 14, 2.

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Culturing Human Cancer and Normal Cell Lines

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Human squamous cell carcinoma (SCC-15) and human glioblastoma (U-118 MG) cell lines obtained from ATCC (Manassas, VA, USA) were cultured in DMEM (doubling time 48 and 35 h, respectively). Normal fibroblast (BJ) purchased from ATCC (doubling time 1.9 days) were grown in EMEM. Each medium was supplemented with 10% heat-inactivated FBS and 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were cultured as described [22 ]. All biological tests were carried out in triplicates in three independent experiments.

Zaręba M., Sareło P., Kopaczyńska M., Białońska A., Uram Ł., Walczak M., Aebisher D, & Wołowiec S. (2019). Mixed-Generation PAMAM G3-G0 Megamer as a Drug Delivery System for Nimesulide: Antitumor Activity of the Conjugate Against Human Squamous Carcinoma and Glioblastoma Cells. International Journal of Molecular Sciences, 20(20), 4998.

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Glioblastoma Multiforme Cell Lines: Characterization and Treatment

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GBM cells (LN18, U87MG, and U118MG) were procured from ATCC (Manassas, VA). LN18 cells were cultured in DMEM supplemented with 5% FBS, U87MG cells in Eagle’s MEM supplemented with 10% FBS, and U118MG cells in DMEM supplemented with 10% FBS. U87MG-LUC cells were constructed by transducing U87MG cells with MSCV Luciferase PGK, and clones were selected with hygromycin. AsA, rutin hydrate, MTT reagent, temozolomide (TMZ), crystal violet, haematoxylin and eosin were from Sigma-Aldrich (St. Louis, MO). Trypan blue was from Invitrogen (Carlsbad, CA). Antibodies for cleaved PARP, cleaved caspases (3, 9, and 8), Bid, Bad, GRP78, IRE1α, Calnexin, PDI, and Calpain were from Cell signaling (Danvers, MA). Survivin antibody was from Novus (Littleton, CO). α-Tubulin antibody was from Neomarkers (Fremont, CA). ECL detection system and anti-mouse HRP conjugated secondary antibody were from GE Healthcare (Buckinghamshire, UK). Protein assay kit was from Bio-Rad Laboratories (Hercules, CA). BAPTA and Fluo-3/AM were from Calbiochem (San Diego, CA). All other reagents were acquired in their highest purity grade available commercially.

Kavitha C.V., Jain A.K., Agarwal C., Pierce A., Keating A., Huber K.M., Serkova N.J., Wempe M.F., Agarwal R, & Deep G. (2014). Asiatic acid induces endoplasmic reticulum stress and apoptotic death in glioblastoma multiforme cells both in vitro and in vivo. Molecular carcinogenesis, 54(11), 1417-1429.

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Human Glioblastoma Cell Culture Protocol

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The human glioblastoma cells, U87MG, U118MG, NU04 and A172 (ATCC, Manassas, VA), were obtained in 2013, and were maintained in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA) with 10 % fetal bovine serum (Thermo Fisher Scientific, Waltham, MA). Cells were grown as monolayer cultures at 37 °C in a humidified atmosphere of 5 % CO₂ and tested for mycoplasma contamination with the Mycoplasma Detection Kit, PlasmoTest (InvivoGen, San Diego, California).

Kyani A., Tamura S., Yang S., Shergalis A., Samanta S., Kuang Y., Ljungman M, & Neamati N. (2018). Discovery and Mechanistic Elucidation of a Class of PDI Inhibitors for the Treatment of Glioblastoma. ChemMedChem, 13(2), 164-177.

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Cell Line Culture Protocols

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The BJ (CRL-2522), Caco-2 (HTB-37), PANC-1 (CRL-1469), U-118 MG (HTB-15), SK-MEL-30 (ACC 151), and MDA-MB-231 (HTB-26) cell lines were purchased from ATCC (Manassas, VA, USA) or DSMZ (Braunschweig, GERMANY). Cells were cultured in accordance with manufacturers' recommendations in a DMEM, MEM, or RPMI-1640 (Sigma, Saint Louis, MO, USA; Gibco, Waltham, MA, USA), supplemented with 10% FBS, except for Caco-2 (20%) (Thermo Fisher Scientific, Waltham, MA, USA) and 1% antibiotic mix solution (penicillin, streptomycin; Thermo Fisher Scientific, Waltham, MA, USA) in a humidified atmosphere with 5% CO₂ at 37 °C. All cell lines were cultured in T75 cell culture flasks or cell culture dishes to reach 80–90% confluency and seeded at a standard density of 5 × 10³ cells/0.32 cm², except for BJ cells at 2 × 10³ cells/0.32 cm².

Baumgart S., Kupczyk D., Archała A., Koszła O., Sołek P., Płaziński W., Płazińska A, & Studzińska R. (2023). Synthesis of Novel 2-(Cyclopentylamino)thiazol-4(5H)-one Derivatives with Potential Anticancer, Antioxidant, and 11β-HSD Inhibitory Activities. International Journal of Molecular Sciences, 24(8), 7252.

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Curcumin Effects on U87MG and U118MG Cells

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The cell lines U87MG and U118MG (ATCC, Rockville, MD, USA) were grown in DMEM supplemented with 10% heat inactivated FBS. All cell lines were grown without antibiotics in an incubator containing humidified atmosphere of 95% air and 5% CO₂ at 37 °C. Curcumin stock solution (20 mM; in DMSO) was kept in a dark colored bottle at −20 °C. Cells were grown to about 70% confluences and then treated with curcumin at different concentrations (0–100 μM) and for different period of time (0–24 h). Cells treated with a medium containing an equivalent amount of DMSO without curcumin was served as control.

Thayyullathil F., Rahman A., Pallichankandy S., Patel M, & Galadari S. (2014). ROS-dependent prostate apoptosis response-4 (Par-4) up-regulation and ceramide generation are the prime signaling events associated with curcumin-induced autophagic cell death in human malignant glioma. FEBS Open Bio, 4, 763-776.

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