4T1, RM-1, HUVEC, and Tohoku Hospital Pediatrics 1 (THP-1) cells (human acute monocytic leukemia cell line) were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). These cell lines were authenticated by their morphology and short tandem repeat genotyping, and tested for mycoplasma. E. coli (ATCC8739) was purchased from Institute of Applied Microbiology, Heilongjiang Provincial Academy of Sciences (Harbin, China). L. reuteri (337178) and B. longum (185971) were purchased from BeNa Culture Collection (Xin yang, China).
Human umbilical vein endothelial cells (huvec)
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
HUVEC is a primary cell line derived from human umbilical vein endothelial cells. It is used for in vitro studies of endothelial cell biology and function.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by National Collection of Authenticated Cell Cultures (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Mcf 7, by National Collection of Authenticated Cell Cultures (3 mentions)
Hct116, by National Collection of Authenticated Cell Cultures (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Smmc 7721, by National Collection of Authenticated Cell Cultures (2 mentions)
Mda mb 231, by National Collection of Authenticated Cell Cultures (1 mentions)
Mcf 10a, by National Collection of Authenticated Cell Cultures (1 mentions)
Dmem medium, by Procell (1 mentions)
Sk br 3, by National Collection of Authenticated Cell Cultures (1 mentions)
Hs578t, by National Collection of Authenticated Cell Cultures (1 mentions)
Hs578t, by Procell (1 mentions)
Thp 1, by National Collection of Authenticated Cell Cultures (1 mentions)
Mcf 10a, by Lonza (1 mentions)
Rpmi 1640 medium, by Procell (1 mentions)
Mda mb 231, by Procell (1 mentions)
Mcf 7, by Procell (1 mentions)
Skbr3, by Procell (1 mentions)
Megm kit, by Lonza (1 mentions)
24 protocols using human umbilical vein endothelial cells (huvec)
1
Cell Line Validation and Procurement
2
Culturing Diverse Human Cell Lines
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The human breast cell line MCF-10 A, human breast cancer cell lines (MCF7, T47D, SKBR-3, MDA-MB-231 and Hs578T), human monocyte cell line THP-1 and human umbilical vein endothelial cell line HUVEC were all purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). T47D, SKBR3, MCF7, MDA-MB-231, Hs578T and HUVEC cells were cultured with DMEM medium (Procell, China) containing 10% fetal bovine serum (FBS). THP-1 cells were cultured with RPMI-1640 medium (Procell, China) with 10% FBS and 0.05 mM ß-Mercaptoethanol. MCF-10 A cells cultured with MEGM kit (Lonza Clonetics, Switzerland). All cells were cultured at 37 °C and in 5% CO2 humidified atmosphere incubator.
3
Evaluating Anticancer Drug Efficacy Using In Vitro Assays
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Reagents and the compounds MTT, DMEM, FBS, and penicillin/streptomycin were all commercially purchased from Nanjing Keybionet Biotechnology Co., Ltd. (Nanjing, China). Hela, HepG2, MCF-7, A549, and HUVEC cells were purchased from the National Collection of Authenticated Cell Cultures.
We added cancer cells to 96-well plates with a density of 1 × 104 cells per well. The culture media were removed after 12 h of incubation at 5% CO2 and 37 °C, and the cells were incubated with DDP, DOX, S1-DDP, and S1-DOX. The standard sample was dissolved in DMEM at different concentrations (each concentration repeated three times) for 48 h at 5% CO2 and 37 °C. Afterward, we removed the culture media and added the new culture medium containing MTT (1 mg/mL), followed by incubation for 4 h. After removing the medium, we added 200 μL of DMSO to each well to dissolve the formazan crystals. Absorbance was measured at 595 nm in a microplate photometer. Cell viability values were determined (at least three times) according to the following formulae: cell viability (%) = the absorbance of the experimental group/the absorbance of the blank control group × 100% [29 (link),30 (link)].
We added cancer cells to 96-well plates with a density of 1 × 104 cells per well. The culture media were removed after 12 h of incubation at 5% CO2 and 37 °C, and the cells were incubated with DDP, DOX, S1-DDP, and S1-DOX. The standard sample was dissolved in DMEM at different concentrations (each concentration repeated three times) for 48 h at 5% CO2 and 37 °C. Afterward, we removed the culture media and added the new culture medium containing MTT (1 mg/mL), followed by incubation for 4 h. After removing the medium, we added 200 μL of DMSO to each well to dissolve the formazan crystals. Absorbance was measured at 595 nm in a microplate photometer. Cell viability values were determined (at least three times) according to the following formulae: cell viability (%) = the absorbance of the experimental group/the absorbance of the blank control group × 100% [29 (link),30 (link)].
4
Investigating miR-30d Inhibitor Effects on Human Cell Lines
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The human cell lines EC109, KYSE150, TE-1, TE-13, and HUVEC were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM medium (Gibco, USA) containing 10% fetal bovine serum. Ricolinostat (ACY-1215) was purchased from Selleck Chemicals (TX, USA) and was dissolved in DMSO to obtain a stock concentration of 100 mM. MiR-30d inhibitor was purchased from RiboBio Company (Guangzhou, China) and was dissolved in RNase-free water to obtain a concentration of 20 µM.
5
Hypoxia-Induced Cardiomyocyte Stress and PRDX2 Overexpression
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Embryonic rat heart-derived H9c2 cells and human umbilical vein endothelial cells (HUVEC) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). H9c2 cardiomyocytes were cultured in high-glucose DMEM supplemented with 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, and 2 mM L-glutamine. Cells were maintained at 37 °C in an atmosphere of 95% air and 5% CO2. After reaching 80–90% confluence, H9c2 cells with or without PRDX2 overexpression were cultured with serum-free medium in a hypoxia chamber (Thermo, Dreieich, Germany) for the indicated time periods. The hypoxic atmosphere was maintained at 37 °C with 2% O2/93% N2/5% CO2. Cells were then removed from the hypoxic chamber and used for further experiments. For other experiments, H9c2 cells exposed to a hypoxic atmosphere for 6 h were used.
6
Cell Line Cultivation Protocols
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HCoEpiC, SW480, HCT116, Caco-2, RKO, HT-29 cell lines, and human umbilical vein endothelial cells (HUVEC) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cell lines HCoEpiC, HCT116, RKO, HT-29, and HUVECs were cultured in PRMI 1640 medium (HyClone, GE Healthcare, UK) containing 10% fetal bovine serum (FBS, HyClone). The Caco-2 and SW480 cell lines were cultured in DMEM medium containing 10% fetal bovine serum. All cells were cultured at 37°C in a 5% CO2 atmosphere.
7
Cell line preparation and ionic extraction
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The NIH/3T3 and HUVEC cell lines were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM medium with 10% fetal bovine serum and 1% penicillin-streptomycin in a CO2 incubator with 5% level at 37 °C. Samples including GelMA, CuNA@GelMA, and CuNA-bFGF@GelMA prepared for making ionic extraction were sterilized by 75% ethanol for 2 h and washed three times with PBS (15 min/time). Ionic extraction of these samples were prepared according to International Standard Organization (ISO/EN) 10993-5 by directly immersing samples into DMEM with 10% FBS at 37 °C in an incubator with 5% CO2 for 24 h.
8
Evaluating Anti-Cancer Effects in Cell Lines
9
Cell Culture of Hepatoma and Control Cells
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Human hepatoma 7402 and SUN-449 cells and noncancerous cell lines, including Raw 264.7 and HUVEC cells, were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). All cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL), and streptomycin (100 μg/mL) in an incubator with 5% CO2 at 37 °C.
10
Culturing Macrophages and Endothelial Cells
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RAW264.7 macrophages and human umbilical vein endothelial cells (HUVEC) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). RAW264.7 cells were cultured in a DMEM medium (Gibco) containing 10% FBS, 1% penicillin (100 mg mL−1), and streptomycin (100 mg mL−1). HUVEC cells were propagated in RPMI 1640 medium (Gibco) containing 10% FBS-containing, 1% penicillin (100 mg mL−1), and streptomycin (100 mg mL−1). All cells were cultured in a 5% CO2 saturated humidity incubator at 37 °C.
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