Log In Sign up
The largest database of trusted experimental protocols

Luminex assay

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The Luminex assay is a multiplex detection system that enables the simultaneous measurement of multiple analytes in a single sample. It utilizes color-coded magnetic beads coated with specific capture antibodies to bind and detect target molecules. The Luminex platform combines flow cytometry and laser technology to provide quantitative results for a wide range of applications, including protein expression analysis, cytokine profiling, and biomarker detection.

Automatically generated - may contain errors

Lab products found in correlation

Mcp 1 elisa kit, by Thermo Fisher Scientific (1 mentions) Labscreen single antigen assay, by Thermo Fisher Scientific (1 mentions) Confocal laser scanning microscope, by Leica (1 mentions) Bio plex system, by Bio-Rad (1 mentions) Legend max elisa kit, by BioLegend (1 mentions) Cytofix cytoperm, by BD (1 mentions) Golgistop, by BD (1 mentions) Il 1β elisa kit, by R&D Systems (1 mentions) Human s100a8 s100a9 heterodimer quantikine elisa kit, by R&D Systems (1 mentions) Luminex assay, by R&D Systems (1 mentions) Elisas, by R&D Systems (1 mentions) Fsl 1, by InvivoGen (1 mentions) Imiquimod, by InvivoGen (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Facscalibur, by BD (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Tnf α, by Miltenyi Biotec (1 mentions) Ccl11, by Thermo Fisher Scientific (1 mentions) Cxcl10, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Luminex Multiplex Assays (13 citations) 63-plex Luminex antibody-conjugated bead capture assay (4 citations) Human Cytokine Magnetic 25-Plex Luminex Assay (4 citations) Luminex assay kits (4 citations) Luminex multiplex human cytokine assay kit (3 citations) BioSource Multiplex Assays for Luminex (2 citations) Luminex cytokine assays (2 citations) Luminex HLA Single Antigen Bead assays (LABScreen; (2 citations) Human 48-plex Luminex Procarta Immuno-assay (2 citations) LabScreen Mixed Luminex assay (2 citations)

34 protocols using luminex assay

1

Comprehensive Cytokine Plasma Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Comprehensive cytokine analysis including 57 plasma cytokines, chemokines, and growth factors (Supplemental Table 2, Page 9) was performed using a magnetic bead based Luminex assay in samples from 14 patients (Affymetrix, CA, USA). Cytokine measurement was part of exploratory analysis.
Dumitriu B., Ito S., Feng X., Stephens N., Yunce M., Kajigaya S., Melenhorst J.J., Rios O., Scheinberg P., Chinian F., Keyvanfar K., Battiwalla M., Wu C.O., Maric I., Xi L., Raffeld M., Muranski P., Townsley D.M., Young N.S., Barrett A.J, & Scheinberg P. (2015). Alemtuzumab in T-cell large granular lymphocytic leukaemia: a phase 2 study. The Lancet. Haematology, 3(1), e22-e29.
+ Open protocol
+ Expand
2

Comprehensive Cytokine Plasma Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Comprehensive cytokine analysis including 57 plasma cytokines, chemokines, and growth factors (Supplemental Table 2, Page 9) was performed using a magnetic bead based Luminex assay in samples from 14 patients (Affymetrix, CA, USA). Cytokine measurement was part of exploratory analysis.
Dumitriu B., Ito S., Feng X., Stephens N., Yunce M., Kajigaya S., Melenhorst J.J., Rios O., Scheinberg P., Chinian F., Keyvanfar K., Battiwalla M., Wu C.O., Maric I., Xi L., Raffeld M., Muranski P., Townsley D.M., Young N.S., Barrett A.J, & Scheinberg P. (2015). Alemtuzumab in T-cell large granular lymphocytic leukaemia: a phase 2 study. The Lancet. Haematology, 3(1), e22-e29.
+ Open protocol
+ Expand
3

Cytokine Analysis of Cytolysis Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Supernatants from the cytolysis assays (see above) were used for cytokine analysis by either Luminex assay (Affymetrix) or ELISA (IL-1β: R&D Systems; IL-18: MBL) as per the manufacturer's instructions.
Whidbey C., Vornhagen J., Gendrin C., Boldenow E., Samson J.M., Doering K., Ngo L., Ezekwe E.A., Gundlach J.H., Elovitz M.A., Liggitt D., Duncan J.A., Adams Waldorf K.M, & Rajagopal L. (2015). A streptococcal lipid toxin induces membrane permeabilization and pyroptosis leading to fetal injury. EMBO Molecular Medicine, 7(4), 488-505.
+ Open protocol
+ Expand
4

Profiling Plasma Chemokine Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma samples were collected in Ethylenediaminetetraacetic acid (EDTA) tubes and stored at -80 C before measuring chemokine levels. Chemokines CCL2, CCL5 and CXCL1 were determined with a Luminex assay (Affymetrix) according to the manufacturer's protocol. Further analysis of plasma CCL2 was performed with a MCP-1 ELISA kit (Thermo Fisher) according to the manufacturer's protocol.
Winter C., Silvestre-Roig C., Ortega-Gomez A., Lemnitzer P., Poelman H., Schumski A., Winter J., Drechsler M., de Jong R., Immler R., Sperandio M., Hristov M., Zeller T., Nicolaes G.A.F., Weber C., Viola J.R., Hidalgo A., Scheiermann C, & Soehnlein O. (2018). Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis. Cell metabolism, 28(1).
+ Open protocol
+ Expand
5

Antibody Profiling in Extracorporeal Photopheresis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All patients in this study had sera obtained before and after initiation of ECP and were screened for Abs to DSA and SAg. DSA determination was performed by the Barnes-Jewish Hospital HLA lab using the LABScreen Single Antigen assay (One Lambda Inc, CA). Results were reported as mean fluorescence intensity values (MFI). In cases where a patient has positive HLA class I and II Abs, the recorded MFI is the sum of both individual MFI measurements.
Abs against SAg (Col-I, Col-V and Kα1T) were measured by an enzyme linked immunosorbent assay (ELISA) and serum levels of cytokines IL-1β, IL-2, IL-4, IL-10, IFN-γ, IL-17 and MCP-1 were measured using the LUMINEX assay (Invitrogen, CA) as per the manufacturer’s protocols and as detailed in our previous publications21 (link),22 (link).
Baskaran G., Tiriveedhi V., Ramachandran S., Aloush A., Grossman B., Hachem R, & Mohanakumar T. (2014). Efficacy of extracorporeal photopheresis in clearance of antibodies to donor and lung specific antigens in lung transplant recipients. The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, 33(9), 950-956.
+ Open protocol
+ Expand
6

Comprehensive Cytokine Profiling by Luminex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sera were thawed and simultaneously analyzed with the multiplex Luminex assay (30-plex, Invitrogen) per manufacturer’s protocol in a BioRad reader (IMCPL). The following analytes were tested: GM-CSF, IFNγ, IP-10, MCP-1, TNFα, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, and IL-10, IL-15,IL-13, IL-17, IL-1Rα, MIP-1β, IL-2r,IL-7 Exotaxin, MCP-1, MIG, MIP-1alpha and RANTES, in a kit pre-tested for any potential cross-reactivity by the manufacturer. Controls included the kit standard curve and Multiplex QC standards (R&D Systems).
Butterfield L.H., Economou J.S., Gamblin T.C, & Geller D.A. (2014). Alpha fetoprotein DNA prime and adenovirus boost immunization of two hepatocellular cancer patients. Journal of Translational Medicine, 12, 86.
+ Open protocol
+ Expand
7

Cytokine and Apoptosis Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Changes in cytokine levels were measured in apical and basal channel effluents from our chips.11 Ten inflammatory cytokines were selected and a Luminex assay was conducted according to the manufacturer’s protocol (Invitrogen). Analyte concentrations were measured using a Luminex FLEXMAP 3D instrument with xPONENT® software. For apoptosis analysis, cleaver Caspase-3 (Cell Signaling Technology, 9661S) was stained with a secondary antibody (ThermoFisher, A31572) and imaged with a laser scanning confocal microscope (Leica); the fluorescence intensity was measured using ImageJ.
Kim S., Naziripour A., Prabhala P., Horváth V., Junaid A., Breault D.T., Goyal G, & Ingber D.E. (2023). Direct therapeutic effect of sulfadoxine-pyrimethamine on nutritional deficiency-induced enteric dysfunction in a human Intestine Chip. eBioMedicine, 99, 104921.
+ Open protocol
+ Expand
8

Cytokine Profiling in Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adherent mouse and human macrophages were incubated at 37°C with 5% CO2 with compound treatments for 24 hours and supernatant collected. For the mouse samples, cytokines were measured using a mouse specific Luminex Assay (Invitrogen, Cat. EPX020-26090-901; GM-CSF, IFNγ, IL1b, IL12p70, IL13, IL18, IL2, IL4, IL5, IL6, TNFα, Eotaxin, Gro-alpha/KC, IP10, Mcp1, Mcp3, Mip1a, Mip1b, Mip2, Rantes) according to manufacturer guidelines. IP10 was measured from the human macrophage supernatant using a human specific CXCL10/IP10 ELISA (R&D Systems Cat. DIP100) according to manufacturer guidelines.
Pardue M.L., DeBaryshe P.G, & Lowenhaupt K. (2001). Another protozoan contributes to understanding telomeres and transposable elements. Proceedings of the National Academy of Sciences of the United States of America, 98(25), 14195-14197.
+ Open protocol
+ Expand
9

Quantification of Soluble Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Soluble factors in 72 h culture supernatants were quantified by Luminex assay (cytokine human magnetic 25-plex panel; Invitrogen, Courtaboeuf, France). IL-6 and IL-8 were also quantified by ELISA (CXCL8 and Human IL-6 Quantikine ELISA Kit, R&D systems) following the manufacturer's instructions.
Benjelloun F., Quillay H., Cannou C., Marlin R., Madec Y., Fernandez H., Chrétien F., Le Grand R., Barré-Sinoussi F., Nugeyre M.T, & Menu E. (2020). Activation of Toll-Like Receptors Differentially Modulates Inflammation in the Human Reproductive Tract: Preliminary Findings. Frontiers in Immunology, 11, 1655.
+ Open protocol
+ Expand
10

Quantifying Soluble Factors in Infected/Uninfected Cocultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Supernatants of infected dMs, infected and uninfected cocultures, infected and uninfected double chamber cocultures and dNK cells were harvested after 48 h of culture. Soluble factors were quantified by Luminex® assay (Cytokine human magnetic 25 plex panel, Invitrogen).
Quillay H., El Costa H., Duriez M., Marlin R., Cannou C., Madec Y., de Truchis C., Rahmati M., Barré-Sinoussi F., Nugeyre M.T, & Menu E. (2016). NK cells control HIV‐1 infection of macrophages through soluble factors and cellular contacts in the human decidua. Retrovirology, 13, 39.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!