Standard bipolar in utero electroporation of the somatosensory cortex was performed as previously described (Bony et al., 2013 (link); Szczurkowska et al., 2016 (link)). Timed-pregnant Sprague Dawley rats (Harlan Italy SRL, Correzzana, Italy) were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (1–2 μg μl−1/Vector in water) and dye Fast Green (0.3 mg ml−1; Sigma, St. Louis, MO) were injected (5–6 μl) through the uterine wall into one of the embryo’s lateral ventricle by a 30-G needle (Pic indolor, Grandate, Italy). Each embryo’s head was held between tweezer-type electrodes (10 mm diameter; Nepa Gene, Chiba, Japan) across the uterus and five electrical pulses (amplitude, 50 V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Tweezer type electrodes
Manufactured by Nepa Gene
Sourced in Japan
The Tweezer-type electrodes are a specialized laboratory tool designed for precise micromanipulation and measurement. They feature a pair of fine, pointed tips that can be used to grasp, hold, and apply electrical signals to small-scale samples or components. The core function of these electrodes is to enable delicate handling and controlled electrical interfacing in various research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tweezer type electrodes
1
In Utero Electroporation of Somatosensory Cortex
2
In Utero Electroporation of Somatosensory Cortex
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