Anti ccr7 150503

Manufactured by R&D Systems

Anti-CCR7 (150503) is a recombinant protein that functions as a chemokine receptor. It is used for research purposes.

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Lab products found in correlation

Streptavidin pacific blue, by Thermo Fisher Scientific (2 mentions) Anti cd25 b1.49.9, by Beckman Coulter (1 mentions) Anti cd8 b9.11, by Beckman Coulter (1 mentions) Antibody coated microbeads, by Miltenyi Biotec (1 mentions) Facsaria, by BD (1 mentions) Nkg2a pe, by Beckman Coulter (1 mentions) Cd28 petexasred, by Beckman Coulter (1 mentions) Cd16 pacific blue, by BD (1 mentions) Cd14 fitc, by BD (1 mentions) Ki67 fitc, by BD (1 mentions) Stat5 pe, by BD (1 mentions) Cd95 pe, by BD (1 mentions)

Close spelling variants of this product

FITC-conjugated anti-CCR7 clone 150503 Anti-CCR7-FITC (clone 150503; Anti-CCR7

2 protocols using anti ccr7 150503

Isolation of CD14+ Monocytes and CD4+ T Cell Subsets

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CD14⁺ monocytes and CD4⁺ T cells were isolated from PBMCs by positive selection with antibody-coated microbeads (Miltenyi Biotec). CD14⁺ monocytes were immediately cryopreserved and stored in liquid nitrogen until required for use as antigen-presenting cells in subsequent stimulation assays. CD4⁺ T cell subsets were cell sorted to 99% purity on a FACSAria (BD) after staining with FITC-labeled anti-CD45RA (ALB11; Beckman Coulter), allophycocyanin-labeled anti-CD4 (SK3; BD), and anti-CCR7 (150503; R&D Systems), followed by staining with biotinylated anti-IgG2a (SouthernBiotech) and streptavidin–Pacific blue (Invitrogen). PE-cyanine 5 (PC5)–labeled anti-CD56 (N901 [NHK-1]; Beckman Coulter), anti-CD25 (B1.49.9; Beckman Coulter), and anti-CD8 (B9.11; Beckman Coulter) were included as a dump channel to exclude natural killer, regulatory, and CD8⁺ T cells.

Campion S.L., Brodie T.M., Fischer W., Korber B.T., Rossetti A., Goonetilleke N., McMichael A.J, & Sallusto F. (2014). Proteome-wide analysis of HIV-specific naive and memory CD4+ T cells in unexposed blood donors. The Journal of Experimental Medicine, 211(7), 1273-1280.

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Flow Cytometry Immunophenotyping Protocol

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The following antibodies were used for flow cytometry: CD3 Alexa 700 (SP34-2 BD Biosciences), CD4 AmCyan (L200 BD Biosciences), CD8 PerCP-Cy5.5 (SKI eBiosciences), CD8 AmCyan (SKI BD Biosciences), CD28 PE-Texas Red (CD28.2 Beckman Coulter, BD Biosciences), CD95 PE (DX2 BD Biosciences, eBiosciences), CCR5 Allophycocyanin (3A9 BD Biosciences), Ki-67 FITC (B56 BD Biosciences), CD56 PerCP-Cy5.5 (MEM-188 Invitrogen), CD16 Pacific Blue (3G8 BD Biosciences, Biolegend), CD20 Allophycocyanin-Cy7 (L27 BD Biosciences), HLA-DR PE-Texas Red (TU36 Invitrogen, Immu357 Beckman Coulter), NKG2A PE (Z199 Beckman Coulter), CD14 FITC (M5E2 BD Biosciences, R&D Systems), STAT5 PE (47/Stat5 (pY6) BD Biosciences), BrdU FITC (B44 BD Biosciences), BrdU Allophycocyanin (B44 BD Biosciences). Anti-CCR7 (150503) was purchased as purified immunoglobulin from R&D Systems, conjugated to biotin using a Pierce Chemical Co. biotinylation kit, and visualized with streptavidin–Pacific Blue (Invitrogen). Rhesus recombinant anti-IL-15 and rhesus recombinant control IgG1 mAb were provided through the National Institutes of Health’s Nonhuman Primate Reagent Resource Program. Simian recombinant IL-7 was provided by Cytheris SA (Issy-Les-Moulineaux, France). Rhesus recombinant IL-15 was provided by Francois Villinger (Emory University) through the Resource for Nonhuman Primate Immune Reagents.

DeGottardi M.Q., Okoye A.A., Vaidya M., Talla A., Konfe A.L., Reyes M.D., Clock J.A., Duell D.M., Legasse A.W., Sabnis A., Park B.S., Axthelm M.K., Estes J.D., Reinmann K.A., Sekaly R.P, & Picker L.J. (2016). Effect of anti-IL-15 administration on T cell and NK cell homeostasis in rhesus macaques. Journal of immunology (Baltimore, Md. : 1950), 197(4), 1183-1198.

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