Formalin

After 28 days, cells were fixed with 10% formalin according to the manufacturer’s protocol (Abcam, San Francisco, CA). Stained cells were imaged using color brightfield microscopy.

Hepatic tissue and epididymal fat were preserved in a 10% formalin (Abcam, USA) solution overnight before being embedded in paraffin (Thermo Fisher Scientific, USA). 4 µm slices were prepared and subsequently deparaffinized with the xylene (Sigma, St Louis, USA) and concentration gradient of ethanol and rehydrated using deionized H₂O for two washes of 5 min. Furthermore, we stained with hematoxylin and eosin (H&E, Harris Hematoxylin and Eosin Y; Sigma, St Louis, USA) (21 (link)), the H&E-stained slides were inspected using a Leica Microsystems CMS GmbH (Wetzlar, Germany), and morphological pictures were analyzed using the SIS 3.2 software (Soft-Imaging System).