Analysis of DM (AOAC method 930.15), CP (AOAC method 988.05), Ca (AOAC method 935.13) and P (AOAC method 965.17) were conducted according to the methods of AOAC (2000) . GE was determined by an automatic adiabatic oxygen bomb calorimeter (Parr 1281 Automatic Energy Analyzer, Moline, IL). P content was analyzed using a UV-visible spectrophotometer (Hitachi, U-1000). Amino acids in diets and digesta were assayed using ion-exchange chromatography according to Wang et al. (2011) (link). Mg, Na, K, Cu, Fe, Mn, and Zn were determined by Inductively-Coupled Plasma Emission Spectroscopy according to Adeola et al. (1995) (link). The IP6 was analyzed on a Dionex DX-500 Ion Chromatograph System (Sunnyvale, CA) with a Dionex CarboPac PA-100 column (4×250 mm) and a Dionex Knitted coil (75 μL) for post column reaction. High performance ion chromatography (HPLC) was used for the analysis of IP6 according to Tran et al. (2011) (link). The determination of chromium content and AID of nutrients followed the method of Wang et al. (2011) (link).
U 1000
Manufactured by Hitachi
Sourced in Japan
The U-1000 is a laboratory spectrophotometer manufactured by Hitachi. It is designed to measure the absorption or transmittance of light by samples across a wide range of wavelengths. The U-1000 provides accurate and reliable data for various applications in scientific research and analysis.
Automatically generated - may contain errors
3 protocols using u 1000
1
Comprehensive Analytical Procedures for Animal Feed
2
Nutrient Composition Analysis of Animal Feeds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Corn, SBM, CM, and all diet samples were analyzed in duplicate for gross energy (GE) with Automatic Isoperibol Oxygen Bomb Calorimeter (Model 6300; Parr Instruments, Moline, IL, USA), dry matter (DM, method 927.05) [16 ], ash (method 942.05) [16 ], CP (method 990.03) [16 ], acid detergent fiber (ADF), and neutral detergent fiber (NDF) (Ankom Technology method 12 and 13, respectively) with Ankom 2000 Fiber Analyzer (Ankom Technology, Macedon, NY, USA), calcium (Ca, method 968.08) [16 ] with an Atomic Absorption Spectrometer (Z-2000, Hitachi, Tokyo, Japan), P (method 931.01) [16 ] with a UV-visible Spectrophotometer after wet-digestion (U-1000, Hitachi, Japan), and phytate [17 (link)], respectively (Tables 1 , 3 ). The concentration of phytate-bound P in each ingredient was calculated as 28.2% of phytate [18 ], and nonphytate-bound P was calculated as the difference between the concentration of total P and phytate-bound P. Fecal samples were also analyzed in duplicate for concentrations of DM, Ca, and P as explained for the ingredients and diets.
3
Colorimetric Analysis of Nitrate and Phosphate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For nitrate and phosphate analysis, samples were filtered through Whatman® GF/F filters using a vacuum pump. The filtered water was frozen at −20 °C until laboratory analysis by the colorimetric methods described in Hansen & Koroleff (1999) using a spectrophotometer Hitachi® U-1000.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!