Hepes

Manufactured by Cultilab

Sourced in Brazil, United States

HEPES is a chemical buffer solution commonly used in cell culture, biochemical, and molecular biology applications. It maintains a stable pH range, typically between 7.2 and 7.5, to support optimal cellular or enzymatic activity. HEPES is an effective buffer for maintaining physiological conditions in various experimental settings.

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Lab products found in correlation

Fetal bovine serum (fbs), by Cultilab (2 mentions) L glutamine, by Cultilab (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) αmem medium, by LGC (1 mentions) Rpmi 1640 medium, by Cultilab (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Bewo cells, by American Type Culture Collection (1 mentions)

2 protocols using hepes

Liposomal Cytotoxicity Evaluation in Murine and Human Cell Lines

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The B16F10 murine melanoma cells (ATCC^® CRL 6475) and human umbilical vein endothelial cells (HUVECs; ATCC^® CRL 1730) were grown in Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich Co.); for Hepa1c1c7 murine hepatocellular carcinoma, ATCC^® CRL 2026 was used as the αMEM medium (LGC Biotecnologia, Cotia, SP, Brazil). The mediums were supplemented with 2 mM L-glutamine (Cultilab, Campinas, SP, Brazil), 10 mM HEPES (Cultilab), 24 mM sodium bicarbonate, 0.01% antibiotics, and 10% fetal bovine serum (Cultilab). Cells were cultivated in 5% CO₂ atmosphere at 37°C as monolayer cultures. Cells were checked for viability using trypan blue exclusion test.
The B16F10 cells and HUVECs were used in this study in order to compare the effects of PHO-S and DODAC/PHO-S liposomes with those from other PHO-S studies, which were recently published and used these same cell lines.4 –8 (link) Hepa1c1c7 was added in this study with the aim of assessing the potential anticancer capability of DODAC/PHO-S liposomes and PHO-S in hepatocarcinoma cells that enables easy reproducibility for in vivo studies. Ethical approval for the use of human cells was not sought.

Luna A.C., Saraiva G.K., Filho O.M., Chierice G.O., Neto S.C., Cuccovia I.M, & Maria D.A. (2016). Potential antitumor activity of novel DODAC/PHO-S liposomes. International Journal of Nanomedicine, 11, 1577-1591.

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Culture and Maintenance of Trophoblast Cell Lines

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Human trophoblast BeWo cells were commercially acquired from American Type Culture Collection (ATCC, Manassas, VA, United States). The human extravillous trophoblast cells (HTR-8/SVneo), originally generated from villous explants an early pregnancy, was gently provided by Dr. Estela Bevilacqua (University of São Paulo, SP, Brazil). BeWo and HTR-8/SVneo cells were maintained in a humidified incubator at 37°C and 5% CO₂ using culture flasks of 75cm². RPMI 1640 medium (Cultilab, Campinas, SP, Brazil) supplemented with 100U/ml of penicillin (Sigma Chemical Co., St. Louis, MO, United States), 100μg/ml of streptomycin (Sigma), 25mM HEPES and 10% fetal bovine serum (FBS; Cultilab) were used for cell culture maintenance (Barbosa et al., 2008 (link)). According to Ethics Committee of the Federal University of Uberlândia, MG, Brazil (Protocol # 13/2012), studies performed with cell lines acquired commercially do not need ethical approval.

Costa I.N., Ribeiro M., Silva Franco P., da Silva R.J., de Araújo T.E., Milián I.C., Luz L.C., Guirelli P.M., Nakazato G., Mineo J.R., Mineo T.W., Barbosa B.F, & Ferro E.A. (2021). Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both Human Trophoblast Cells and Villous Explants. Frontiers in Microbiology, 11, 623947.

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