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Silver flakes

Manufactured by Merck Group
Sourced in United States

Silver flakes are a form of pure metallic silver in a flake-like shape. They are primarily used as a conductive material in various applications, such as in the production of electronic components, coatings, and inks. The core function of silver flakes is to provide electrical conductivity and reflective properties.

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6 protocols using silver flakes

1

Ecoflex-based Flexible Biosensor

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Ecoflex 00–30 was purchased from Smooth-on. TPU film was purchased from Lubrizol. Silver flakes, SEBS, toluene, Prussian blue (soluble), chitosan, acetic acid, potassium hydroxide (KOH), PVA (MW ~89,000), phosphate buffer solution (PBS) (1 M, pH 7.4), uric acid, l-lactic acid, d(+)-glucose, acetaminophen, ascorbic acid, sucrose, sodium chloride, bovine serum albumin (BSA) and potassium chloride were purchased from Sigma-Aldrich. Graphite powder was purchased from Acros Organics. Super-P carbon black was obtained from MTI. Silver conductive epoxy adhesive was purchased from MG Chemicals. LOx (activity 101 U mg−1) was purchased from Toyobo. Mould release spray (Smooth-on) was purchased from Amazon.
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2

Thermoreversible Hydrogel for Drug Delivery

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Mebiol Gel PNIPAAm–PEG 3D thermoreversible hydrogel was obtained from Cosmo Bio (Tokyo, Japan). Chitosan, gold(III) chloride hydrate (HAuCl4), thioglycolic acid (TGA), potassium phosphate (dibasic), sodium acetate, acetic acid, doxorubicin hydrochloride (DOX), mitomycin C, and silver flakes were purchased from Sigma-Aldrich (St. Louis, MO). Phosphate-buffered saline (PBS), Dulbecco’s modified Eagle’s medium (DMEM), MEM nonessential amino acid solution (MEM NEAA), fetal bovine serum (FBS), TrypLE Express enzyme, and penicillin–streptomycin (Pen–Strep) were obtained from ThermoFisher Scientific (Carlsbad, CA). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) was obtained from Carbosynth (San Diego). N-Succinimidyl acrylate (NHS-AA) and N-hydroxysuccinimide (NHS) were purchased from TCI America (Portland, OR). Ethyl alcohol was obtained from Pharmco-AAPER (Shelbyville, KY). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Alfa Aesar (Haverhill, MA). Paclitaxel (PTX) and docetaxel (DTX) were obtained from Avachem Scientific (San Antonio, TX). All distilled water used in this study was generated using a Thermo Scientific Barnstead GenPure Water Purification System (Waltham, MA).
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3

Synthesis of Stretchable Silver Ink

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Example 1

Fluoroelastomer (DAI-EL G801, Daikin Industries) and butanone (Sigma Aldrich) were mixed with weight ratio of 1:2.55. After stirring with a magnetic stirrer for 6 hours, silver flakes (Sigma Aldrich, average particle size of 2-3.5 μm, >99.9% trace metals basis) were added to the solution in a 2.5:1 (silver:fluoroelastomer) weight ratio and mixed with magnetic stirrer for 4 h. 10 minutes sonication was applied to the solution to get well-dispersed stretchable silver ink. All procedures are carried out at room temperature.

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4

Inductive Wireless Charging via Conductive Ink

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The polymer solution was prepared by dissolving 5% Alginate powder (Sigma-Aldrich) in deionized water followed by centrifuging for 2 min at a rate of about 7155 × g to remove air bubbles. The conductive ink was prepared by adding silver flakes (with an average diameter of 10 µm, Sigma-Aldrich) and ethanol into the Alginate solution in the weight ratio of 4:6:1. The square resistivity of the ink film was measured with four-point probing equipment (ST2558B-F01, Suzhou Jingge Electronic co., Ltd.). Through wireless power transmission, an alternating magnetic field was generated by an electromagnetic coil with a power of 1600 W. An LED connected to the conductive silver structures was used to demonstrate the inductive currents generated by the alternating magnetic field.
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5

Synthesis of Castor Oil-based Polyurethane

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Poly(tetramethylene ether) glycol (Mw = 1000 g/mol, 98%) and castor oil (CO, Mw = 933.45, 90% ricinoleic acid) have been obtained from Sigma-Aldrich and refluxed for 3 h at 120 °C before being used. Soybean oil (SBO, Sigma-Aldrich), aliphatic isophorone diisocyanate (IPDI, 98%, Alfa Aesar), formic acid (HCOOH, ≥95%, Sigma-Aldrich), and N, N-dimethylformamide (DMF, 99.5%, Thermo Scientific™) were used without additional distillation. The diethyl ether (Et2O, ≥99.5%), dibutyltin dilaurate (DBTDL, 95%), DMG (≥99%), silver flakes (<10 µm), lactic acid (~90%), sodium chloride (≥99%), acetic acid (≥97%), citric acid monohydrate (≥98%), lipase (from Candida rugosa) were purchased from Sigma-Aldrich and used in their original forms. Stretchable TPU substrate and textile Lycra Shiny Milliskin Nylon Spandex Fabric was purchased from Spandex World. Inc, USA, and Dongguan Jiaxin Plastic Materials, respectively. Cambridge Isotope Laboratories Inc. provided deuterated solvents for NMR characterization. Double-deionized water was used throughout the study.
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6

Attaching gold wire to stink bugs

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To attach the gold wire onto the pronota of the stink bugs, adult females were immobilized using an adhesive tape (PL Mitchell, pers. comm.) on the top of a Petri dish lid, then attached to the posterior part of the abdomen. The gold wire (3 cm long, and 0.1 mm in diameter) (Sigmund Cohn, Mount Vernon, NY, USA) was glued to a copper electrode (3 cm long) that had previously been soldered to a brass nail; together, the reusable copper wire plus brass nail are termed a 'wiring stub'. At the tip of the gold wire, a small loop (0.5 mm) was made to expand the surface of gold area in contact with the stink bug pronotum using conductive silver glue. This glue was made by mixing water, silver flakes (Sigma-Aldrich, Saint Louis, MO, USA), and white glue (Elmer's Glue-All, Westerville, OH, USA) (1:1:1 vol/wt/vol). A drop of the silver glue was placed on the centre of the pronotum, and the loop of the gold wire was sunk into the glue, where it was allowed to dry for 40 min. After that, the bugs were released (the adhesive tape was carefully removed to avoid damaging the bug) and were allowed to dangle from the wiring stub for 10 min before they were used with the EPG monitor.
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