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Porcine c peptide elisa

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The Porcine C-peptide ELISA is a quantitative sandwich enzyme immunoassay designed for the measurement of C-peptide in porcine samples. It utilizes antibodies specific for porcine C-peptide and provides a reliable method for the detection and quantification of this analyte.

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Lab products found in correlation

Vitros 5.1 fs system, by Ortho Clinical Diagnostics (1 mentions)

Close spelling variants of this product

ELISAs (9 citations) Mercodia C-peptide ELISA (6 citations) C-peptide (6 citations)

6 protocols using porcine c peptide elisa

1

Porcine Islet Transplantation Evaluation

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The function of the transplanted islets was assessed by monitoring the blood glucose and plasma porcine C-peptide concentrations. Normoglycemia was defined as a blood glucose concentration of <200 mg/dL. The plasma porcine C-peptide concentrations were measured using a Porcine C-peptide ELISA (Cat#10-1256-01; Mercodia, Winston Salem, NC, USA).
Sakata N., Yoshimatsu G., Kawakami R., Aoyagi C, & Kodama S. (2023). Optimal temperature for the long-term culture of adult porcine islets for xenotransplantation. Frontiers in Immunology, 14, 1280668.
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2

Bovine Lung Aprotinin Treatment and C-peptide Measurement

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UF was collected and treated with bovine lung aprotinin (Millipore-Sigma, Darmstadt, Germany) at a ratio of a minimum of 500 kU to 1 ml of sample. C-peptide was measured via commercial Porcine C-Peptide ELISA (Mercodia, Uppsala, Sweden) (106 ).
Oppler S.H., Hocum Stone L.L., Leishman D.J., Janecek J.L., Moore M.E., Rangarajan P., Willenberg B.J., O’Brien T.D., Modiano J., Pheil N., Dalton J., Dalton M., Ramachandran S, & Graham M.L. (2024). A bioengineered artificial interstitium supports long-term islet xenograft survival in nonhuman primates without immunosuppression. Science Advances, 10(1), eadi4919.
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3

Evaluating Islet Graft Function

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The function of the transplanted islets was assessed by monitoring blood glucose and plasma porcine C-peptide concentrations, and changes in blood glucose concentration during glucose tolerance testing. Normoglycemia was defined as a blood glucose concentration < 200 mg/dL. Plasma porcine C-peptide concentrations were measured using a porcine C-peptide ELISA (Mercodia, Winston Salem, NC, USA).
Sakata N., Yoshimatsu G., Kawakami R, & Kodama S. (2024). Influence of relatively short-term culture on adult porcine islets for xenotransplantation. Scientific Reports, 14, 11640.
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4

Monitoring Islet Transplant Outcomes

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Post-transplantation monitoring consisted of daily clinical assessment by the veterinary staff, as well as weekly laboratory studies including complete blood count and complete metabolic panel. Rhesus CMV was monitored using quantitative polymerase chain reaction from whole blood weekly and when clinically indicated. Intramuscular ganciclovir at 6mg/kg was given as treatment if recipient serum CMV exceeded 10,000 copies/ml.
Following streptozocin induction, animal morning fasting and afternoon postprandial blood glucose levels were monitored daily by either ear- or tail-stick. Based on blood glucose readings, animals received NPH insulin twice daily and long-acting lantus once in the evening per sliding scale to maintain a target fasting blood glucose (FBG) <200 mg/dL. Intravenous glucose tolerance tests (IVGTTs) with a bolus of dextrose (500 mg/kg) were performed pre-diabetes, pre-transplant, and then every two weeks during the post-transplantation period. IVGTT glucose levels were measured 5 minutes prior to dextrose bolus, immediately following dextrose bolus, and then at 10-, 30-, 60- and 90-min intervals. Porcine c-peptide was measured from sera obtained at each IVGTT timepoint and from serial samples obtained throughout the study period using the Mercodia Porcine C-peptide ELISA (Mercodia, Uppsala, Sweden).
Gao Q., Davis R., Fitch Z., Mulvihill M., Ezekian B., Schroder P., Schmitz R., Song M., Leopardi F., Ribeiro M., Miller A., Moris D., Shaw B., Samy K., Reimann K., Williams K., Collins B, & Kirk A. (2021). Anti-thymoglobulin Induction Improves Neonatal Porcine Xenoislet Engraftment and Survival. Xenotransplantation, 28(6), e12713.
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5

Intravenous Glucose Tolerance Test for Diabetes

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An intravenous glucose tolerance test (IVGTT) was performed under full GA prior to the animal being rendered diabetic, after induction of diabetes, and post-transplant at monthly intervals. An intravenous bolus of dextrose (0.5 g/kg body weight) was injected into the cephalic venous line followed by a saline flush. Blood samples were obtained before injection and at 1, 2.5, 3, 5, 7.5, 10, 15, 20, 30, 40, 45, 50, 60, 75, 90, 105 and 120 min after injection. Blood glucose concentrations were measured using a dry chemistry method on a Vitros 5.1/FS system (Ortho-Clinical Diagnostics, Rochester, NY). NHP and porcine insulin were measured using Mercodia Insulin ELISA and c-peptide concentrations were measured using a Mercodia Porcine C-peptide ELISA (Mercodia AB, Uppsala, Sweden). K values [percentage decline of natural logarithm (blood glucose)/min] were calculated with blood glucose measurements from the IVGTT.
Hawthorne W.J., Salvaris E.J., Chew Y.V., Burns H., Hawkes J., Barlow H., Hu M., Lew A.M., Nottle M.B., O’Connell P.J, & Cowan P.J. (2022). Xenotransplantation of Genetically Modified Neonatal Pig Islets Cures Diabetes in Baboons. Frontiers in Immunology, 13, 898948.
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6

Porcine C-Peptide ELISA for Graft Function

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To assess graft function, the level of porcine C-peptide was measured in the serum of NICC graft recipients using Mercodia porcine C-peptide ELISA according to the manufacturer’s instructions.
Zhao Y., Nicholson L., Wang H., Qian Y.W., Hawthorne W.J., Jimenez-Vera E., Gloss B.S., Lai J., Thomas A., Chew Y.V., Burns H., Zhang G.Y., Wang Y.M., Rogers N.M., Zheng G., Yi S., Alexander S.I., O’Connell P.J, & Hu M. (2024). Intragraft memory-like CD127hiCD4+Foxp3+ Tregs maintain transplant tolerance. JCI Insight, 9(6), e169119.
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