The conjugated antibodies used for functional molecules detection were the following: PerCP-Cy5.5 Mouse Anti-Human CD3 (catalog: 560835), FITC Mouse Anti-Human CD16 (catalog: 561308), PE Mouse Anti-Human NKp46 (catalog: 557991), PE Mouse Anti-Human NKp44 (catalog: 558563), PE Mouse Anti-Human NKG2A (catalog: 555889), PE Mouse Anti-Human CD158a (catalog: 556063), PE Mouse Anti-Human CD158b (catalog: 559785), PE Mouse Anti-Human Perforin (catalog: 556437), PE Mouse Anti-Human Granzyme B (catalog: 561142), APC Mouse Anti-Human CD11c (catalog: 559877), PE Mouse Anti-Human CD80 (catalog: 557227), PE Mouse Anti-Human CD86 (catalog: 555658), PE-Cy7 Mouse Anti-Human CD56 (catalog: 557747), Mouse Anti-Human CD86 (catalog: 555658) and FITC Annexin V Apoptosis Detection Kit (catalog: 556570) were purchased from BD Pharmingen (Franklin Lakes, USA). PE Mouse Anti-Human CD314 (NKG2D)(catalog: 12-5879-42), APC Mouse Anti-Human TIM3 (catalog: 17-3109-42), PerCP Mouse Anti-Human HLA-DR (catalog: MA1-10348) were purchased from Invitrogen (Carlsbad, USA).
Pe mouse anti human cd158b
Manufactured by BD
Sourced in United States
The PE Mouse Anti-Human CD158b is a laboratory reagent used for the detection and identification of the CD158b antigen, also known as killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3), on the surface of human cells. It is a monoclonal antibody conjugated with the fluorescent dye R-Phycoerythrin (PE).
Automatically generated - may contain errors
Lab products found in correlation
Pe mouse anti human cd158a, by BD (2 mentions)
Fitc mouse anti human cd16, by BD (1 mentions)
Pe mouse anti human cd86, by BD (1 mentions)
Pe cy7 mouse anti human cd56, by BD (1 mentions)
Pe mouse anti human cd80, by BD (1 mentions)
Percp cy 5.5 mouse antihuman cd3, by BD (1 mentions)
Mouse anti human cd86, by BD (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)
Ficoll paque plus, by Miltenyi Biotec (1 mentions)
Apc cy7 anti human cd4 antibody, by BioLegend (1 mentions)
Pe cy7 antihuman cd3 antibody, by BioLegend (1 mentions)
Percp cyanine5.5 anti human cd19, by BioLegend (1 mentions)
Fcr cd16 32 blocking reagent, by Miltenyi Biotec (1 mentions)
Apc cyanine7 streptavidin, by BioLegend (1 mentions)
Pe cyanine7 streptavidin, by BioLegend (1 mentions)
Pe anti human cd184 cxcr4 antibody, by BioLegend (1 mentions)
Arial 2, by BD (1 mentions)
2 protocols using pe mouse anti human cd158b
1
Functional Molecules Detection in Immune Cells
2
Immunophenotyping of Peripheral Blood and Bone Marrow Cells
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PB and BM samples from HDs and AA patients were collected in EDTA anticoagulant tubes and stored at 4 °C. PBMCs and BMMCs were isolated by density gradient centrifugation using Ficoll‐Paque PLUS within 24 h. PBMCs and BMMCs were blocked using FcR (CD16/32) Blocking Reagent (Miltenyi), and stained with the following antibodies/reagents: PE anti‐human CD158f (KIR2DL5) antibody (Biolegend), PE anti‐human CD158e1 (KIR3DL1, NKB1) antibody, PE mouse anti‐Human CD158a (BD Pharmingen), PE mouse anti‐human CD158b (BD Pharmingen), APC anti‐human TCRγ/δ (Biolegend), Biotin anti‐humanTCRvδ2 (Biolegend), APC/Cyanine7 streptavidin (Biolegend), human KIR3DL2/CD158k PE‐conjugated antibody (R&D), anti‐humanCD261(DR4)‐APC (Miltenyi), FITC anti‐human CD8a antibody (Biolegend), APC‐Cy7 anti‐human CD4 antibody (Biolegend), Percp‐Cy5.5 anti‐human CD25 antibody (Biolegend), PE‐Cy7 anti‐human CD127 antibody (eBioscience), PE/Cyanine7 Streptavidin (Biolegend), PerCP/Cyanine5.5 anti‐human CD19 (BioLegend), PE‐Cy7 anti‐human CD3 antibody (BioLegend), APC anti‐human TCRγ/δ antibody (BioLegend), and PE anti‐human CD184 (CXCR4) antibody (BioLegend). The cells were resuspended in 400 µl 0.1 µg mL−1 DAPI solution (Salarbio). Treg cells (CD8a−CD4+CD127−/lowCD25+) were sorted using an Arial II (BD Biosciences).
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