(POPC), egg sphingomyelin (SM), cholesterol (Ch), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)(ammonium salt) (Rho-DOPE) were
purchased from Avanti Polar Lipids (Alabaster, AL). Poly(1,2-butadiene)-b-poly(ethylene oxide) (PBD22-b-PEO14) with the average molecular weights of 1200 and
600 g/mol for the PBD and PEO blocks, respectively, was purchased
from Polymer Source Inc. (Dorval, Quebec, Canada). Sucrose, glucose,
and chloroform were purchased from Sigma-Aldrich (St. Louis, MO). N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium
salt (NBD-PE) and 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose
(2-NBDG) were purchased from ThermoFisher Scientific (Eugene, OR).
Sucrose, glucose, and 2-NBDG solutions were prepared in deionized
water (DI) (Millipore, Sigma, St. Louis, MO) were used for experimentation.