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2 protocols using triethylammoniumsalt nbd pe

1

Lipid Membrane Composition and Labeling

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1-Palmitoyl-2-oleoylglycero-3-phosphocholine
(POPC), egg sphingomyelin (SM), cholesterol (Ch), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)(ammonium salt) (Rho-DOPE) were
purchased from Avanti Polar Lipids (Alabaster, AL). Poly(1,2-butadiene)-b-poly(ethylene oxide) (PBD22-b-PEO14) with the average molecular weights of 1200 and
600 g/mol for the PBD and PEO blocks, respectively, was purchased
from Polymer Source Inc. (Dorval, Quebec, Canada). Sucrose, glucose,
and chloroform were purchased from Sigma-Aldrich (St. Louis, MO). N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium
salt (NBD-PE) and 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose
(2-NBDG) were purchased from ThermoFisher Scientific (Eugene, OR).
Sucrose, glucose, and 2-NBDG solutions were prepared in deionized
water (DI) (Millipore, Sigma, St. Louis, MO) were used for experimentation.
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2

Cell Viability Assay Protocol

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Paraformaldehyde, dimethyl sulfoxide (DMSO), phosphate buffered saline (PBS), Amiodarone and compounds listed in Table 1 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified eagle medium (DMEM), penicillin/streptomycin solution (100X) were purchased from HyClone Laboratories (Logan, UT, USA). Hoechst33342, fetal bovine serum (FBS), MEM non-essential amino acids (100X), L-glutamine and N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium Salt (NBD-PE) were purchased from Thermo Fisher Scientific (St. Wyman, MA, USA). Insulin, transferrin and dexamethasone were purchased from Solarbio (Beijing, China). Epidermal growth factor was purchased from Pepro Tech (Rocky Hill, NJ, USA). Cell Counting Kit-8 was obtained from Dojindo Molecular Technologies (Kumamoto, Japan). Glutaraldehyde was purchased from SPI Supplies Division of Structure Probe (West Chester, PA, USA).
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