The assay was designed to detect complexes containing DNA and leucocyte calprotectin and performed as previously described [40 (link)]. In brief, 96-well microplates (ThermoFisher Scientific, Waltham, MA, USA) were coated with rabbit anti-histone 3 (H3) (Agrisera AB, Vännäs, Sweden) or anti-calprotectin (ThermoFisher Scientific, Waltham, MA, USA), the latter used to establish a standard curve for bound calprotectin in complexes with H3. Plasma samples were tested at 1:5 dilution. After incubation at room temperature (RT) for 90 min and three washes, the horse radish peroxidase (HRP)-conjugated monoclonal anti-calprotectin antibodies were added to the wells. The H3-NET amount was measured as 450 nm and expressed in ng/mL.
Anti calprotectin
Manufactured by Thermo Fisher Scientific
Sourced in Sweden, United States
Anti-calprotectin is a lab equipment product used for the detection and measurement of calprotectin, a protein involved in various inflammatory processes. The product provides a reliable and accurate method for quantifying calprotectin levels in biological samples, supporting research and diagnostic applications.
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Lab products found in correlation
2 protocols using anti calprotectin
1
Quantifying H3-Calprotectin Complexes
2
Quantifying Intestinal Inflammation Biomarkers
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The expression of calprotectin and mast cell tryptase was determined via immunohistochemical analysis. The paraffin-embedded histological tissue sections from the ileum and proximal colon were deparaffinized. Tissue sections were incubated in 3% hydrogen peroxide for 10 minutes to block activity of endogenous peroxidase. The tissue sections were then incubated overnight with primary antibodies, anti-calprotectin (1:250; ThermoFisher, Waltham, MA, USA) and anti-mast cell tryptase (1:2000; ThermoFisher), 4°C. After 3 washes with phosphate-buffered saline, the tissue sections were incubated with secondary antibody anti-mouse IgG (1:200; Vector Laboratories). Next, the sections were incubated with streptavidin-HRP for 30 minutes and then treated with AB-peroxidase solution and counterstaining with hematoxylin. Images were analyzed via MetaMorph (MDS Analytical Technologies, Sunnyvale, CA, USA).
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