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Cd8α apc efluor780 clone 53 6.7

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The CD8α-APC-eFluor780 (clone 53-6.7) is a fluorescently labeled antibody that binds to the CD8α molecule. CD8α is a glycoprotein expressed on the surface of certain T cells and natural killer cells. The antibody is conjugated to the fluorescent dyes APC-eFluor780, which can be used to detect and analyze CD8α-positive cells in flow cytometry applications.

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Lab products found in correlation

Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Lsr 2, by BD (2 mentions) Cd5 fitc clone 53 7.3, by BD (2 mentions) Cd44 pe cy7 clone im7, by BD (2 mentions) Cd4 qdot605 clone rm4.5, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (2 mentions)

Close spelling variants of this product

CD8α (clone 53–6.7, CD8α (53-6.7, Clone 53-6.7 CD8α-APC

2 protocols using cd8α apc efluor780 clone 53 6.7

1

Multiparameter Flow Cytometry of Thymic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
FTOCs or thymic slices were dissociated into single cell suspensions, filtered,
and stained with antibodies for cell surface markers. The following antibodies were used
for FTOC samples: CD5-FITC (clone 53-7.3) and CD44-PE-Cy7 (clone IM7) from BD Biosciences;
TCRβ-APC (clone H57–597), CD24-Pacific Blue (clone M1/69), and
TCRγδ-PerCPCy5.5 (clone GL3) from BioLegend; CD25-PE (clone PC61.5) and
CD8α-APC-eFluor780 (clone 53-6.7) from eBioscience, and CD4-Qdot605 (clone RM4.5)
from Life Technologies. The following antibodies were used for thymic slice samples:
CD45.1-FITC (clone A20), CD69-PerCPCy5.5 (clone H1.2F3), CD5-PE (clone 53-7.3), CD4-PE-Cy7
(clone RM4–5), CD8α-eFluor450 (clone 53-6.7) from eBioscience. For
negative selection experiments, cells were pre-stained with LIVE/DEAD Fixable Aqua Dead
Cell Stain kit (Molecular Probes) for thirty minutes in PBS. Data were acquired on an
LSRII or LSR Fortessa (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Au-Yeung B.B., Melichar H.J., Ross J.O., Cheng D.A., Zikherman J., Shokat K.M., Robey E.A, & Weiss A. (2014). Quantitative and Temporal Requirements Revealed for Zap-70 Catalytic Activity During T Cell Development. Nature immunology, 15(7), 687-694.
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2

Multiparameter Flow Cytometry of Thymic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
FTOCs or thymic slices were dissociated into single cell suspensions, filtered,
and stained with antibodies for cell surface markers. The following antibodies were used
for FTOC samples: CD5-FITC (clone 53-7.3) and CD44-PE-Cy7 (clone IM7) from BD Biosciences;
TCRβ-APC (clone H57–597), CD24-Pacific Blue (clone M1/69), and
TCRγδ-PerCPCy5.5 (clone GL3) from BioLegend; CD25-PE (clone PC61.5) and
CD8α-APC-eFluor780 (clone 53-6.7) from eBioscience, and CD4-Qdot605 (clone RM4.5)
from Life Technologies. The following antibodies were used for thymic slice samples:
CD45.1-FITC (clone A20), CD69-PerCPCy5.5 (clone H1.2F3), CD5-PE (clone 53-7.3), CD4-PE-Cy7
(clone RM4–5), CD8α-eFluor450 (clone 53-6.7) from eBioscience. For
negative selection experiments, cells were pre-stained with LIVE/DEAD Fixable Aqua Dead
Cell Stain kit (Molecular Probes) for thirty minutes in PBS. Data were acquired on an
LSRII or LSR Fortessa (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Au-Yeung B.B., Melichar H.J., Ross J.O., Cheng D.A., Zikherman J., Shokat K.M., Robey E.A, & Weiss A. (2014). Quantitative and Temporal Requirements Revealed for Zap-70 Catalytic Activity During T Cell Development. Nature immunology, 15(7), 687-694.
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