Genesys 20 uv vis spectrophotometer

Manufactured by Thermo Fisher Scientific

Sourced in United States

The GENESYS 20 UV–VIS spectrophotometer is a compact and reliable instrument designed for general laboratory use. It features a dual-beam optical system and a wavelength range of 190 to 1100 nm. The GENESYS 20 is capable of measuring the absorbance, transmittance, and concentration of various samples.

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Lab products found in correlation

2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions) Prestoblue assay, by Thermo Fisher Scientific (1 mentions) Hptlc silica gel 60 f254 plate, by Merck Group (1 mentions) Simplicity system, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions) Tlc silica gel 60 f254 plate, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions)

Close spelling variants of this product

UV-Vis spectrophotometer (180 citations) Genesys 20 (154 citations) UV spectrophotometer (112 citations) Genesys 20 spectrophotometer (67 citations) GENESYS 20 UV−VIS (2 citations) Genesys UV-VIS spectrophotometer (2 citations) Spectrophotometer Genesys (2 citations)

5 protocols using genesys 20 uv vis spectrophotometer

Glucose Consumption Rate Measurement

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Glucose consumption rate has
been estimated based on the monitored glucose level in culture medium
by the BioMaxima-glucose enzymatic assay (BioMaxima, PL). 1.0 mL of
BioMaxima reagent was mixed with 20 μL of filtered (syringe
filters, ϕ = 0.2 μm) culture medium gathered from the
cultures (in the case of the test sample) or with 20 μL of double-distilled
water (in the case of the blank sample). Next, all samples were incubated
for 20 min at room temperature before absorbance measurements using
a GENESYS 20 UV–VIS spectrophotometer (Thermo Fisher Scientific,
US) at 550 nm. Based on absorbance, the glucose concentrations were
calculated. Finally, values of the glucose consumption rate normalized
by the average concentration of glucose were determined according
to the following equation: where r_glc is the glucose consumption
rate and C_glc, i and C_glc, j are
the glucose concentrations in the culture medium
at time stamps i and j (for j > i).

Ulatowski K., Wierzchowski K., Fiuk J, & Sobieszuk P. (2022). Effect of Nanobubble Presence on Murine Fibroblasts and Human Leukemia Cell Cultures. Langmuir, 38(28), 8575-8584.

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Spectrophotometric LDH Activity Assay

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LDH activity
has been determined according to the procedure of BioMaxima-LDH enzymatic
assay (BioMaxima, PL). 1.0 mL of Biomaxima-LDH reagent was added to
20 μL of filtered (syringe filters, ϕ = 0.2 μm)
culture medium sampled from cultures or mixed with 20 μL of
double-distilled water (in the case of blank samples). Absorbances
of such reaction mixtures were spectrophotometrically monitored in
1 min intervals using a GENESYS 20 UV–VIS spectrophotometer
(Thermo Fisher Scientific, US) at 340 nm. Finally, values of LDH activity
were estimated based on the following correlation, proposed by the
assays’ manufacturer: where a_LDH is the lactate dehydrogenase activity and ΔA is the absorbance change per minute. Afterward, this activity
was normalized using cell density to show the leakage of LDH per cell, a_LDH/X as follows:

Ulatowski K., Wierzchowski K., Fiuk J, & Sobieszuk P. (2022). Effect of Nanobubble Presence on Murine Fibroblasts and Human Leukemia Cell Cultures. Langmuir, 38(28), 8575-8584.

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Metabolic Activity Quantification via PrestoBlue Assay

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Metabolic activity assessment was based on the PrestoBlue assay results (Thermo Fischer Scientific, US). 156 μL of PrestoBlue reagent was added to 1.5 mL of L929 cells culture or cell-free culture (reference). All samples were incubated at 37°C for 2 h. Then, absorbance was measured using a GENESYS 20 UV−VIS spectrophotometer (Thermo Fisher Scientific, US) at 570 and 600 nm. Metabolic activity was calculated according to the formula:

metabolic activity = 37.04 \times (A_{570} - A_{570 R E F}) - (A_{600} - A_{600 R E F}), [μ kat / {dm}^{3}]

where:
A₅₇₀—the absorbance of the test sample at 570 nm, A_570REF–the absorbance of the reference sample at 570 nm, A₆₀₀—the absorbance of the test sample at 600 nm, A_600REF–the absorbance of the reference sample at 600 nm.
Results from three trials were averaged. The value of the standard deviation was calculated.

Bandzerewicz A., Howis J., Wierzchowski K., Slouf M., Hodan J., Denis P., Gołofit T., Pilarek M, & Gadomska-Gajadhur A. (2024). Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing. Frontiers in Bioengineering and Biotechnology, 12, 1332290.

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Echinacea Root Antioxidant Characterization

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The basic raw materials for the preparation of feed were obtained from ANIMEX Group S.A., Zamosc Branch. Dried Echinacea root was obtained from “A Herbal Farm Waldemar Lupa”, Poland.
Standards of rutin, gallic, cichoric, caftaric and caffeic acids, echinacoside as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH), were purchased from Sigma–Aldrich Fine Chemicals (St. Louis, MO, USA).
Solvents used for HPLC were HPLC-grade, purchased from J.T. Baker (the Netherlands), and water was purified using a Millipore laboratory ultra pure water system (Simplicity™ system, Millipore, Molsheim, France). Methanol used for preparation of the extracts was of analytical grade and obtained from the Polish Reagents (POCH, Gliwice, Poland).
The TLC–DPPH test was performed on the HPTLC silica gel 60 F254 plates (Merck, Darmstadt, Germany). The extracts were prepared using ultrasonic bath (Bandelin Electronic, Sonorex RK 100H, Germany). Samples were applied to chromatographic plates with the applicator Desaga AS-30 (Heidelberg, Germany). Absorbance was measured by a GENESYS™ 20 UV–Vis spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) in a 1 cm quartz cell.

Oniszczuk T., Oniszczuk A., Gondek E., Guz L., Puk K., Kocira A., Kusz A., Kasprzak K, & Wójtowicz A. (2016). Active polyphenolic compounds, nutrient contents and antioxidant capacity of extruded fish feed containing purple coneflower (Echinacea purpurea (L.) Moench.). Saudi Journal of Biological Sciences, 26(1), 24-30.

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DPPH Antioxidant Activity Assay

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Quercetin and DPPH were obtained from Sigma-Aldrich Fine Chemicals (St. Louis, MO, USA). Analytical purity grade methanol, chloroform, ethyl acetate, and formic acid were of chromatographic grade from Merck (Darmstadt, Germany). The TLC−DPPH test was conducted on TLC silica gel 60 F₂₅₄ plates, 20 × 20 cm (Merck). Extraction was performed using an ultrasonicator bath (Bandelin Electronic, Sonorex RK 100H, Germany). Antioxidant activity was determined using a Genesys 20 UV−vis spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA) in a 1 cm quartz cell.

Mayasari D., Murti Y.B., Pratiwi S.U., Sudarsono S., Hanna G, & Hamann M.T. (2021). TLC-Based Fingerprinting Analysis of the Geographical Variation of Melastoma malabathricum in Inland and Archipelago Regions: A Rapid and Easy-to-Use Tool for Field Metabolomics Studies. Journal of natural products, 85(1), 292-300.

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