Transfection of SLAMF3 was performed with pBud-SLAMF3 plasmid in Huh-7 cells, as previously described by Marcq et al. [17 (link)]. Transfection with empty pBud plasmid served as a negative control. For SLAMF3 ectopic expression, 2 × 105 of Huh-7 Ctrl or Res cells were first seeded into six-well plates for 24 h prior to transfection. Cells were transfected with 0.8 μg of plasmid DNA using the FuGENE HD Transfection Reagent Kit (Promega, Charbonnières-les-bains, France) according to the manufacturer’s instructions, and were incubated for 48 h or 72 h at 37 °C before all experiments.
Fugene hd transfection reagent kit
Manufactured by Promega
Sourced in France
The FuGENE HD Transfection Reagent Kit is a non-liposomal transfection reagent designed for efficient delivery of DNA, RNA, or protein into a variety of cell types. The kit includes the transfection reagent and necessary buffers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fugene hd transfection reagent kit
1
SLAMF3 Overexpression in Huh-7 Cells
2
Constructs and Transfection of CAS and Cortactin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Constructs for WT CAS (wild type Crk-associated substrate) and CAS-SD (nonphosphorylatable Crk-associated substrate mutant) have been previously described [21 (link), 35 (link)]. Constructs for WT cortactin (wild type cortactin) and NP cortactin (nonphosphorylatable cortactin mutant) have been previously described [26 (link)]. Cell transfection was performed by using the FuGENE HD transfection reagent kit (Promega) according to the manual of the manufacture.
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