Anti rpa32 s33

Western blotting was performed as previously described (15 (link)). The primary antibodies were used at the following dilutions: anti-PTIP (1:1000, Bethyl), anti-CtIP (1:1000, Proteintech), anti-CtIP (1:500, Santa Cruz), anti-RPA32-S33 (1:1000, Bethyl), anti-β-actin (1:3000, Abclonal), anti-FALG (1:2000, Invitrogen), anti-HA (1: 1000, Proteintech), anti-UFL1 (1:1000, Bethyl), anti-GST (1:1000, Abclonal), and anti-His (1:1000, Abclonal).

Cells grown on coverslips were prepared for IF by standard procedures. The following antibodies were used for immunostaining: anti-ATRX (Santa Cruz sc-15408); anti-ATRX 39f [38] (link); anti MRE11 (Abcam ab214); anti-MRE11 (Calbiochem PC388); anti-RAD50 (Abcam ab89); anti-PCNA (Santa Cruz sc-9857); anti-RPA32 (S33) (Bethyl A300-246A); anti-NBS1 (BD Biosciences 611871); anti-53BP1 (Novus Biologicals NB100-305); For MRN co-localisation studies cells were pre-permeabilised prior to fixation with ice cold 0.5% Triton X-100. For MRE11/ATRX/PCNA co-localisation in mES cells, cells were attached to coverslips via poly-l-lysine treatment prior to pre-permeabilisation. Telomere FISH was performed subsequent to antibody incubation using Telomere PNA FISH Kit/Cy3 (Dako K5326).