Alinity c analyzer

Serum adiponectin, leptin, resistin, complement factor D, monocyte chemoattractant protein 1 (CCL2), Serpin (PAI-1), IL-6, IL-10, TNF-alpha (TNF-a) were measured using an automated immunoassay platform called ELLA (ProteinSimple, Bio-techne, Minneapolis, MN, USA) [15 (link)].
Serum concentrations of IGF-I, IGFBP-3, and SHBG were determined by a chemiluminescent immunoassay designed for the IDS-iSYS Multi-Discipline Automated System (Immunodiagnostic Systems Limited, United Kingdom).
Serum concentrations of insulin were determined by a chemiluminescent microparticle immunoassay, performed on the automated instrument ARCHITECT i System (Abbott Laboratories, Wiesbaden, Germany). Serum concentrations of C-reactive protein were determined by an immunoturbidimetric assay using the automated instrument ALINITY c analyzer (Abbott Laboratories, Wiesbaden, Germany). HOMA-IR [fasting insulinemia (mU/L) x glycemia (mmol/L)]/22.5 was applied as a surrogate index of insulin resistance.

Cells were plated in a 6-well plate at a density of 200,000 cells/well and treated after 24 h with H₂O₂ for additional 24 h. A minimum of 200 µL of the culture medium was removed for lactate quantification, which was performed by using the Alinity Lactic Acid Reagent Kit (8P2120, Abbott, Chicago, IL, USA) following the manufacturers’ instructions. The reaction was measured photometrically with the Alinity c analyzer (Abbott, Chicago, IL, USA). Measured lactate concentrations were normalized to the total protein amount per well.

Three different validated methods were used for the analytical assay, as each hospital had a different system. One hospital used a chemiluminescent microparticle immunoassay with an Architect i4000SR immunoassay analyzer (Abbott), two hospitals used the kinetic interaction of microparticles in solution (KIMS) with a Cobas c system (Roche/Hitachi), and three hospitals used the homogeneous particle-enhanced turbidimetric inhibition immunoassay (PETINIA) with an Alinity c analyzer (Abbott). The assay range for the Architect immunoassay is from 0.5 to 83 µg/ml, the range for the KIMS assay is 4–80 µg/ml, and the range for the PETINIA is 1.4–100 µg/ml. For the Scr assays, four centers used the Jaffe method, and two centers used the enzymatic method. The following formula was used to convert the enzymatic assay to the Jaffe method (22 (link)): $Jaffeconcentration=0.122+{\displaystyle \frac{Enzymaticconcentration}{1.05}}$