Somatostatin

Manufactured by GeneTex

Sourced in United States

Somatostatin is a hormone produced in the hypothalamus and pancreas that regulates the secretion of other hormones, such as growth hormone and insulin. It plays a role in inhibiting the release of these hormones, thereby helping to maintain homeostasis in the body.

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Lab products found in correlation

Glucagon, by Abcam (2 mentions) Insulin, by Agilent Technologies (1 mentions) Dmire2, by Leica (1 mentions) Volocity software, by PerkinElmer (1 mentions)

3 protocols using somatostatin

Immunofluorescent Pancreatic Cell Staining

Cited 1 time

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Mouse pancreata were fixed in 4% paraformaldehyde and embedded with paraffin. Rehydrated 5 μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300, GeneTex., Irvine, CA, USA), and glucagon (dilution 1:100; Abcam, Cambridge, UK) followed by secondary antibodies (dilution 1:500; anti-guinea pig Alexa Fluor 488, dilution 1:500; anti-mouse, Alexa Fluor 647, and dilution 1:500; anti-rabbit Alexa Fluor 546) as previously described (Vierra et al., 2015 (link)). Sections were imaged either with a Nikon Eclipse TE2000-U microscope or fluorescent ScanScope (Aperio).

Nakhe A.Y., Dadi P.K., Kim J., Dickerson M.T., Behera S., Dobson J.R., Shrestha S., Cartailler J.P., Sampson L., Magnuson M.A, & Jacobson D.A. (2024). The MODY-associated KCNK16 L114P mutation increases islet glucagon secretion and limits insulin secretion resulting in transient neonatal diabetes and glucose dyshomeostasis in adults. eLife, 12, RP89967.

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Immunofluorescent Imaging of Pancreatic Islets

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Mouse pancreata were fixed in 4% paraformaldehyde and embedded with paraffin. Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300, GeneTex., Irvine, CA, USA), and glucagon (dilution 1:100; Abcam, Cambridge, United Kingdom) followed by secondary antibodies (dilution 1:500; anti-guinea pig Alexa Fluor 488, dilution 1:500; anti-mouse, Alexa Fluor 647, and dilution 1:500; anti-rabbit Alexa Fluor 546) as previously described(14 (link)). Sections were imaged either with a Nikon Eclipse TE2000-U microscope or fluorescent ScanScope (Aperio).

Nakhe A.Y., Dadi P.K., Kim J., Shrestha S., Cartailler J.P., Sampson L., Magnuson M.A, & Jacobson D.A. (2023). The MODY-associated TALK-1 L114P mutation causes islet α-cell overactivity and β-cell inactivity resulting in transient neonatal diabetes and glucose dyshomeostasis in adults. bioRxiv.

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Islet and β-cell Imaging with Confocal Microscopy

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Islet and β-cell imaging was performed with a Leica laser scanning confocal microscope system (DMIRE2; Leica Microsystems, Wetzlar, Germany) with ×20 (numerical aperture 0.75) and ×60 (numerical aperture 1.4) oil immersion objectives as previously described (17 (link)). Primary antibodies include insulin (guinea pig, 1:200; Dako Diagnostics, Mississauga, Ontario, Canada), glucagon (mouse, 1:200; NovoCastra Laboratories, Leica Microsystems), and somatostatin (rabbit, 1:200; GeneTex Inc., Irvine, CA) and rabbit anti–Syt-7 antibody (1:200; Synaptic System). Deconvolution algorithm was applied to images to remove out-of-focus background noise. Colocalization was quantified using Volocity software (PerkinElmer, Waltham, MA). Regions of interest (ROIs) were outlined; the number of colocalized pixels within a particular ROI was determined and expressed as percentage of total pixels within that ROI.

Dolai S., Xie L., Zhu D., Liang T., Qin T., Xie H., Kang Y., Chapman E.R, & Gaisano H.Y. (2016). Synaptotagmin-7 Functions to Replenish Insulin Granules for Exocytosis in Human Islet β-Cells. Diabetes, 65(7), 1962-1976.

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