Z vad fmk c1202

Manufactured by Beyotime

Sourced in China

Z-VAD-FMK (C1202) is a broad-spectrum caspase inhibitor. It inhibits the enzymatic activity of caspases, which are key regulators of apoptosis, or programmed cell death. Z-VAD-FMK acts as a potent, cell-permeable, and irreversible inhibitor of caspase-1, -3, -6, -7, -8, and -9.

Automatically generated - may contain errors

Lab products found in correlation

Mg132 s1748, by Beyotime (1 mentions) Tlrl chq, by InvivoGen (1 mentions) Poly 1 c tlrl pic, by InvivoGen (1 mentions) Ab18766, by Abcam (1 mentions) Ab125066, by Abcam (1 mentions) Cuso4, by Chron Chemicals (1 mentions) Df4255, by Affinity Biosciences (1 mentions) N acetylcysteine nac a9165, by Merck Group (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Beyotime (1 mentions)

3 protocols using z vad fmk c1202

Investigating the Role of HSP27 and OAS1 in Viral Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The empty vectors pCMV-HA and pCMV-Myc were provided by the Biomedical Research Center of Northwest Minzu University. The recombinant plasmids Myc-HSP27, Myc-OAS1, Flag-MDA5, HA-VP1, HA-VP2, HA-2A, HA-2C, HA-3A, Myc-VP3, and Myc-3C were all constructed in the lab. The proteasome inhibitor MG132 (S1748) and caspase inhibitor Z-VAD-FMK (C1202) were obtained from Beyotime (Shanghai, China). The autolysosome inhibitor chloroquine (tlrl-chq) and poly (I:C) (tlrl-pic) were obtained from InvivoGen (San Diego, CA, United States). The small interfering RNA (siRNAs) an analog of double-stranded RNA (dsRNA) targeting homo HSP27 were designed and synthesized by Guangzhou Ribo Biotechnology (China). The siRNA sequences are listed in Table 1. A549 cells in 6-well plates were transfected with siRNAs or various plasmids using Lipofectamine™ 2000 according to the manufacturer’s instructions.

Li X., Ma R., Wu B., Niu Y., Li H., Li D., Xie J., Idris A, & Feng R. (2021). HSP27 Protein Dampens Encephalomyocarditis Virus Replication by Stabilizing Melanoma Differentiation-Associated Gene 5. Frontiers in Microbiology, 12, 788870.

+ Open protocol

+ Expand

Investigating Autophagy and Apoptosis Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

CuSO₄ came from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). N-acetylcysteine (NAC) (A9165) and 3-MA (M9281) were provided by Sigma Aldrich. Z-VAD-FMK (C1202) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) (S0033 M) were provided by Beyotime Biotechnology. Compound C (Com C) (S7306) was purchased from Selleck.
Antibodies involved in the current research included: rabbit anti-LC3B (2775S CST), rabbit anti-p62 (5114S CST), rabbit anti-AMPKα (5832 CST), rabbit anti-p-AMPKα (2535 CST), rabbit anti-mTOR (2972 CST), rabbit anti-p-mTOR (2971 CST), rabbit anti-ULK1 (8054 CST), rabbit anti-p-ULK1(Ser757) (6888 CST), rabbit anti-Atg16L1 (8089T CST), rabbit anti-Atg7 (8558 CST), rabbit anti-Atg3 (3415 CST), rabbit anti-Beclin1 (12994 CST), anti-rabbit cleaved-caspase-3 (9664 CST), anti-rabbit cleaved-caspase-8 (9496 CST), anti-rabbit cleaved-caspase-9 (9509 CST), anti-rabbit caspase-12 (ab18766 Abcam), and anti-rabbit cleaved-PARP (5625 CST), anti-rabbit GPX4 (ab125066 Abcam), anti-rabbit COX-2 (abs131986 Absin Biotechnology), anti-rabbit FTH1 (bs-5907R Bioss Biotechnology) and anti-rabbit NCOA4 (DF4255 Affinity biosciences).

Guo H., Ouyang Y., Yin H., Cui H., Deng H., Liu H., Jian Z., Fang J., Zuo Z., Wang X., Zhao L., Zhu Y., Geng Y, & Ouyang P. (2021). Induction of autophagy via the ROS-dependent AMPK-mTOR pathway protects copper-induced spermatogenesis disorder. Redox Biology, 49, 102227.

+ Open protocol

+ Expand

Antibody Reagents for Cellular Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse monoclonal anti-human SOD1 (sc-101523), SOD2 (sc-137254), BRCA1 (sc-6954) and BRCA2 (sc-518154) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-human caspase 3 (9662), PARP (9532), HO-1 (5853) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). ROS scavengers N-acetyl cysteine (NAC, ST1546) and reduced glutathione (GSH, S0073), DCF ROS assay kit (S0033), pan-caspase inhibitor Z-vad-FMK (C1202), and a mouse monoclonal anti-human GAPDH antibody (AF5009) were purchased from Beyotime (Shanghai, China). PARP inhibitor PJ34 (3255), were purchased from Tocris (Ellisville, MO, USA). SOD1 inhibitor LCS-1 (567417) was purchased from Merck (Darmstadt, Germany). Recombinant human EGF (AF-100-15), IL-6 (200-06) were purchased from PeproTech (Rocky Hill, NJ, USA).

Ling M., Liu Q., Wang Y., Liu X., Jiang M, & Hu J. (2022). LCS-1 inhibition of superoxide dismutase 1 induces ROS-dependent death of glioma cells and degradates PARP and BRCA1. Frontiers in Oncology, 12, 937444.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!