Dulbecco’s modified Eagle’s medium (DMEM) and Ham’s F-10 medium were obtained from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan). Penicillin/streptomycin and trypsin–EDTA were purchased from Thermo Fischer Scientific (Rochester, NY, USA). Cell culture equipment and rectangular 8-well plates were obtained from BD Biosciences (San Jose, CA, USA) and Thermo Fisher Scientific, respectively. SUMILON Cell-disk LF1 was from Sumitomo Bakelite Co. LTD (Tokyo, Japan). Insert chamber (Falcon 353,095–353,097) was purchased from Corning (Lowell, MA, USA) and an ultrasonic cutter (ZO-91, Echo Tech Co. Ltd, Japan) was used to make an incision (~ 3 mm) for conducting electricity at the sidewall located ~ 5 mm above the bottom (Fig. S1 ). The insert chamber was placed between the carbon electrodes, one of which directly faced the incision. Calf serum (CS) and fetal bovine serum (FBS) were obtained from BioWest (Nuaille, France). Matrigel was obtained from Corning (#354230, NY, USA). Unless otherwise noted, all chemicals were of the purest grade available from Sigma Chemical (St Louis, MO, USA) or Fujifilm Wako Pure Chemical Corp.
Ham s f 10 medium
Manufactured by Fujifilm
Sourced in Japan
Ham's F-10 medium is a cell culture medium used for the in vitro cultivation of various cell types. It is a balanced salt solution that provides the necessary nutrients and ingredients to support the growth and maintenance of cells in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Matrigel, by Corning (2 mentions)
Fetal bovine serum (fbs), by Biowest (2 mentions)
Calf serum, by Biowest (2 mentions)
Cell culture equipment, by BD (2 mentions)
Rectangular 8 well plates, by BD (2 mentions)
Dulbecco s modified eagle s medium dmem, by Fujifilm (2 mentions)
Sumilon cell disk lf1, by Sumitomo Bakelite (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Nacalai Tesque (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Hek blue tgf β cells, by InvivoGen (1 mentions)
Glutamine, by Nacalai Tesque (1 mentions)
293 t and 293ft cells, by Thermo Fisher Scientific (1 mentions)
Non essential amino acid solution, by Nacalai Tesque (1 mentions)
3 protocols using ham s f 10 medium
1
Electrical Stimulation of Cell Cultures
2
Cell Culture Media and Reagents
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Dulbecco’s modified Eagle’s medium (DMEM), and Ham’s F-10 medium were purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan). Penicillin/streptomycin, and trypsin–EDTA were obtained from Thermo Fischer Scientific (Rochester, NY, USA). Cell culture equipment and rectangular 8-well plates were obtained from BD Biosciences (San Jose, CA, USA) and Thermo Fisher Scientific, respectively. SUMILON Cell-disk LF1 was from Sumitomo Bakelite Co. LTD (Tokyo, Japan). Calf serum (CS) and fetal bovine serum (FBS) were obtained from BioWest (Nuaille, France). Matrigel was obtained from Corning (#354230, NY, USA). Unless otherwise noted, all chemicals were of the purest grade available from Sigma Chemical (St Louis, MO, USA) or Fujifilm Wako Pure Chemical Corp.
3
Cell Culture of Melanoma and Kidney Cell Lines
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The B16-F0 mouse melanoma cell line (cat. no. JCRB0202) was purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank and maintained in Eagle's Minimum Essential Medium (EMEM; FUJIFILM Wako Pure Chemical Corporation) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich; Merck KGaA), 100 U/ml penicillin and 100 µg/ml streptomycin (both from Nacalai Tesque, Inc.) under mycoplasma-free conditions. Clone M3 (Cloudman S91) melanoma cell line was obtained from European Collection of Authenticated Cell Cultures (ECACC) and cultured in Ham's F10 medium (FUJIFILM Wako Pure Chemical Corporation) supplemented with 15% FBS, 2 mM glutamine (Nacalai Tesque, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin. 293T and 293FT cells were obtained from Invitrogen; Thermo Fisher Scientific, Inc. HEK-Blue™ TGF-β cells were purchased from InvivoGen. 293T, 293FT and HEK-Blue™ TGF-β cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Inc.) supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. The cultured medium for 293FT cells was also supplemented with 1% non-essential amino acid solution (Nacalai Tesque, Inc.). All cell lines were cultured in a humidified incubator containing 5% CO2 at 37°C.
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