The largest database of trusted experimental protocols

Cd160

Manufactured by BD
Sourced in United States

CD160 is a lab equipment product. It is a surface receptor that is expressed on natural killer cells, cytotoxic T cells, and a subset of T cells. The core function of CD160 is to facilitate cell-cell interactions and signal transduction.

Automatically generated - may contain errors

3 protocols using cd160

1

Investigating SRSF2 Function in Tumor-Infiltrating Lymphocyte Immune Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
To investigate the functions of SRSF2 in the immune response of TILs against autologous tumor cell lines, TILs transfected with SRSF2 siRNA were co-cultured with autologous tumor cell lines. Then, flow cytometry was performed with an anti-CD8 antibody (Biolegend, 344704) and anti-IFN-γ antibody (BD Biosciences, 554552) on BD AccuriC6 (BD Biosciences). To examine the expression level of the immune checkpoints in the TILs and PBMCs, the frequency of immune checkpoint + and CD8 + TILs was assessed using flow cytometry on BD AccuriC6 (BD Biosciences). The antibodies against the immune checkpoints used for flow cytometry included PD-L1 (BD Biosciences, 561787), BTLA (BD Biosciences, 564802), CTLA-4 (BD Biosciences, 561717), LAG3 (BD Biosciences, 565617), and CD160 (BD Biosciences, 562351). All data were analyzed by FlowJo software.
+ Open protocol
+ Expand
2

Phenotyping Cryopreserved PBMCs by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved PBMCs were thawed in a 37 C water bath as quickly as possible and were washed twice with RPMI 1640 medium to remove the dimethyl sulfoxide. The samples were used for flow cytometry analysis. The PBMCs were immunolabelled using antibodies against the following surface proteins: CD3, CD56, CD28, and CD160 (BD Biosciences, Franklin Lake, USA) according to the manufacturer's instructions. Flow cytometry was carried out using Fluorescence Activated Cell Sorting flow cytometers (Beckman Coulter FC50, Life Sciences, Indianapolis, USA). Data acquisition was carried out using Cell Quest software (BD Biosciences, Franklin Lake, USA).
+ Open protocol
+ Expand
3

Multiparametric Flow Cytometry Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry, fluorochrome-conjugated antibodies against the following antigens were used: CD56 (clone NCAM16.2; conjugated to BV605) (B159; APC), CD3 (SK7; BUV395) (HIT3a; PE), CD107a (H4A3; APC), IFN-γ (B27; PerCP/Cy5.5), TNF (MAb11; PE), CD69 (FN50; BV421 and BV711), CD11a (HI111; BV711), NKG2D (149810; PE-CF594), NKp30 (p30-15; BV421), TACTILE (6F9; BV711), TRAIL (RIK-2; BV421), BTLA (J168-540; PE-CF594), Phospho-Lck (4/LCK-Y505; AF647), Phospho-LAT (J96-1238.58.93; PE), Phospho-SLP-76 (J141-668.36.58; PE), Phospho-MEK1/2 (O24-836; PE), Phospho-ERK1/2 (20A; BV421), Phospho-SHP-2 (L99-921; AF647) (all BD Biosciences), CD160 (BY55; PE/Cy7), NKp46 (9E2; PE/Cy7),NKp44 (P44-8; PE), DNAM-1 (11A8; APC), 2B4 (C1.7; PerCP/Cy5.5), NKp80 (5D12; PE) (all BioLegend), NKG2C (134591; APC) (R&D Systems), and VZV gE:gI (SG1-1; conjugated in-house to DyLight 488) (Meridian Life Science). Matched isotype control antibodies were also used where appropriate.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!