Dynamic light scattering (DLS) and zeta potential measurements were conducted using a Zetasizer Nano ZS (Malvern). Scanning electron microscopy (SEM) was conducted using a Zeiss Supra 25 Field Emission scanning electron microscope. SEM samples were prepared by gold sputtering and critical point drying. For AFM measurements, nanoparticles were observed on a freshly cleaved mica surface using an Asylum MFP 3D Bio AFM with an Olympus AC240TS probe. For transmission electron microscopy (TEM), a carbon-coated copper TEM grid (Ted Pella) was used with a JEOL 1200 EX transmission electron microscope operated at 80 kV.
1200 ex transmission
Manufactured by JEOL
Sourced in United States
The JEOL 1200 EX is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of samples. It utilizes an electron beam to transmit through thin specimens, providing detailed information about the sample's internal structure, composition, and morphology at the nanoscale level. The JEOL 1200 EX is a versatile instrument suitable for various applications in materials science, life sciences, and other fields that require advanced microscopic analysis.
Automatically generated - may contain errors
Lab products found in correlation
Supra 25 field emission scanning electron microscope, by Zeiss (2 mentions)
Mfp 3d bio afm, by Olympus (2 mentions)
Ac240ts probe, by Olympus (2 mentions)
Amt image capture engine v602, by Advanced Microscopy Techniques (1 mentions)
X cite 120 led light source, by Excelitas (1 mentions)
Elements software, by Nikon (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Rat anti lamp1 antibody, by Abcam (1 mentions)
Ultracut s microtome, by JEOL (1 mentions)
Amt 8 megapixel digital camera, by Advanced Microscopy Techniques (1 mentions)
Aqueous uranyl acetate, by Ted Pella (1 mentions)
J 810 spectropolarimeter, by Jasco (1 mentions)
8 protocols using 1200 ex transmission
1
Nanoparticle Characterization via Multimodal Analysis
2
Microscopy Imaging of Bacterial Morphology
3
Nanoparticle Characterization via Multimodal Analysis
4
Immunogold Labeling of LAMP1 in Cells
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Raw264.7 cell infected as described above were fixed in 4% paraformaldehyde/0.05% glutaraldehyde in 100mM PIPES/0.5mM MgCl2, pH 7.2 for 1 hr at 4°C, were embedded in 10% gelatin and then infiltrated overnight with 2.3M sucrose/20% polyvinyl pyrrolidone in PIPES/MgCl2 at 4°C. Samples were trimmed, frozen in liquid nitrogen, and sectioned with a Leica Ultracut FC7 cryo-ultramicrotome (Leica Microsystems Inc., Bannockburn, IL). Ultrathin sections of 65 nm were blocked with 5% FBS/5% NGS for 30 min and subsequently incubated with rat anti-LAMP1 antibody (Abcam, cat.#ab25245) for 1 hr at room temperature. Following washes in block buffer, sections were incubated with 18nm colloidal gold-conjugated goat anti-rat IgG (H+L) (Jackson Immuno-Research Labs, cat.#112-005-003) for 1 hr. Sections stained with 0.3% uranyl acetate/2% methyl cellulose and microscopy performed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc., Peabody, MA) equipped with an AMT 8 megapixel digital camera and AMT Image Capture Engine V602 software. All labeling experiments were conducted in parallel with controls omitting the primary antibody.
5
Corpus Callosum Axon Myelination Analysis
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Brains, harvested after anesthesia and sequential cardiac perfusion with 15 ml/mouse of 0.09% NaCl containing heparin (25 mg/ml) and 4% PFA in 0.1 M cacodylate buffer, were post-fixed in 2% PFA 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 30 min at room temperature. A brain matrix was used to dissect the region encompassing the midline of the corpus callosum. 1-mm sections were oriented to generate cross-sections of axons within the corpus callosum. Ultrathin sections were cut and stained with toluidine blue. A minimum of 10 images that showed axons cut in cross-section were obtained at 5K, 10K and 20K magnification for quantitation. Images were analyzed by Photoshop software using the free hand selection tool and manually thresholded to determine the number of myelinated axons, axon cross-sectional area, axon diameter (caliber) and g-ratios. 300 axons/mouse were analyzed from 10 non-serial sections from 10K images. An axon was counted as unmyelinated if the area of the axon was equal to or larger than the area of the smallest myelinated axon in the corresponding field. Images were acquired on a JEOL 1200 EX transmission electron microscope.
6
Ultrastructural Analysis of Lgr5-lacZ Colon
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Two Lgr5-lacZ male mice, 6–8 weeks old, were used per time point. Colonic samples were fixed in 2.5% glutaraldehyde plus 2% paraformaldehyde in 0.075 M sodium cacodylate buffer for 1 hour at 4°C and postfixed in 2% osmium tetroxide in double-distilled water. Samples were then rinsed in double-distilled water and dehydrated in a graded alcohol series of 50%, 75%, and 95% through absolute alcohol, followed by propylene oxide, with overnight incubation in 1:1 propylene oxide/poly bed 812 epoxy resin (manufacturer unknown). Ultrathin sections, obtained with a Reichert Ultracut S microtome, were stained with uranyl acetate and lead citrate and photographed using a Jeol 1200 EX transmission electron microscope. Experiments were performed in the Electron Microscopy Facility at the Sloan Kettering Institute.
7
Bacterial Visualization by Negative Staining TEM
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For negative staining and analysis by transmission electron microscopy, bacterial suspensions were allowed to absorb for 10 min onto freshly glow-discharged Formvar/carbon-coated copper grids. The grids were washed in distilled water and stained for 1 min with 1% aqueous uranyl acetate (Ted Pella Inc., Redding, CA). Excess liquid was gently removed, and grids were air dried. Samples were viewed on a JEOL 1200EX transmission electron microscope (JEOL USA, Peabody, MA) equipped with an AMT 8-megapixel digital camera (Advanced Microscopy Techniques, Woburn, MA).
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Time-Resolved Microscopy and Spectroscopy
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