Transmission electron microscopy (Morgani 268D; Philips, Eindhoven, The Netherlands) was used to measure the size of isolate P7213 after negative staining at an operating voltage of 60 kV, as previously described34 (link). For that, a total of seven mycobacteria was measured.
Morgani 268d
Manufactured by Philips
The Morgani 268D is a laboratory centrifuge designed for a variety of applications. It features a high-speed motor capable of reaching up to 16,000 RPM and can accommodate a range of sample volumes and tube sizes. The centrifuge is equipped with a robust, well-balanced rotor system to ensure efficient and reliable separation of samples.
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4 protocols using morgani 268d
1
Transmission Electron Microscopy of Mycobacteria
2
Characterization of Bacillus andreraoultii
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Bacillus andreraoultii strain SIT1T was observed, after negative colouration, using a Morgani 268D (Philips, Amsterdam, The Netherlands) transmission electron microscope at an operating voltage of 60 kV. The Gram colouration was performed using Color Gram 2 Kit (bioMérieux) and observed by using the DM1000 photonic microscope (Leica Microsystems, Wetzlar, Germany) with a 100× oil-immersion objective lens. The sporulation test was done doing a thermic shock (80°C during 30 minutes). To evaluate the motility of Bacillus andreraoultii, fresh colonies were observed between blades and slats using a DM1000 photonic microscope (Leica) with a 40× objective lens.
API ZYM, API 20 NE and API 50 CH (bioMérieux) gallery systems were used to perform biochemical assays. Oxidase (Becton Dickinson, Franklin Lakes, NJ, USA) and catalase assays (bioMérieux) were done separately. The antibiotic susceptibility was tested using SirScan Discs antibiotics (i2a, Montpellier, France).
API ZYM, API 20 NE and API 50 CH (bioMérieux) gallery systems were used to perform biochemical assays. Oxidase (Becton Dickinson, Franklin Lakes, NJ, USA) and catalase assays (bioMérieux) were done separately. The antibiotic susceptibility was tested using SirScan Discs antibiotics (i2a, Montpellier, France).
3
Polymersome Visualization via TEM
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For visualization, 10 μL of a polymersome solution was negatively stained with 2% aqueous uranyl acetate solution, deposited on a carbon-coated copper grid, and then examined with a transmission electron microscope (Philips Morgani 268 D) operating at 80 kV.
4
Microorganism Size Determination by TEM
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The size of the microorganisms was determined by transmission electron microscopy (Morgani 268D; Philips, Eindhoven, The Netherlands) after negative staining at an operating voltage of 60 kV.
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