IL-2 (Miltenyi Biotec); IL-21, IL-6, IFN-α, TGF-β3, SDF1α, Osteoprotegerin, M-CSF, Osteopontin, SCF, MIF, IGF-BP2, IGF-II, and Activin A (PeproTech); Jagged-1, ANGPT1, VEGF-165 and VEGF-121 (R&D Systems); Multimeric-APRIL (Caltag); goat anti-human IgM & IgG F(ab′)2 fragments (Jackson ImmunoResearch); Hybridomax hybridoma growth supplement (Gentaur); Lipid Mixture 1, chemically defined (200×) and MEM Amino Acids Solution (50×) (Sigma-Aldrich); SB525334 (Selleckchem).
Hybridomax hybridoma growth supplement
Manufactured by Gentaur
HybridoMax is a hybridoma growth supplement. It is designed to support the growth and development of hybridoma cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 6 (il 6), by Thermo Fisher Scientific (3 mentions)
Mem amino acids solution, by Merck Group (3 mentions)
7 aad, by BD (3 mentions)
Il 21, by Thermo Fisher Scientific (2 mentions)
Cd19 pe lt19, by Miltenyi Biotec (2 mentions)
Cd138 allophycocyanin b b4, by Miltenyi Biotec (2 mentions)
Cd38 af700, by BioLegend (2 mentions)
Cd20 efluor v450 2h7, by Thermo Fisher Scientific (2 mentions)
Cd27 af647 lt27, by Bio-Rad (2 mentions)
Cd19 percp cy5.5 sj225c1, by BD (2 mentions)
Cd24 fitc ml5, by BD (2 mentions)
Cd27 bv605, by BioLegend (2 mentions)
Cd3viogreen bw264 56, by Miltenyi Biotec (2 mentions)
Unconjugatedgoat anti irf4 m 17, by Santa Cruz Biotechnology (2 mentions)
Donkey anti goat igg af488, by Thermo Fisher Scientific (2 mentions)
Goat anti human f ab 2 fragments anti igm and igg, by Jackson ImmunoResearch (2 mentions)
Ifn α, by Merck Group (2 mentions)
Lipid mixture 1, by Merck Group (2 mentions)
Cd38 pe cy7, by BD (2 mentions)
Human il 2, by Roche (2 mentions)
4 protocols using hybridomax hybridoma growth supplement
1
Optimal Cytokine Conditions for Cell Culture
2
Multiparametric Flow Cytometry of B Cells
3
Comprehensive Multicolor Flow Cytometry for Plasma Cell Analysis
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Abs used were CD19 PE (LT19; Miltenyi Biotec), CD138 allophycocyanin (B-B4; Miltenyi Biotec), CD38 PE-Cy7 (HB7; BD Biosciences), CD38 AF700 (HIT2; BioLegend), CD20 eFluor V450 (2H7; eBioscience); CD27 AF647 (LT27; AbD Serotec), CD27 FITC (M-T271), CD19 PerCP-Cy5.5 (SJ225C1; BD Biosciences), CD19 PE-Cy7 (SJ225C1; BD Biosciences), CD24 FITC (ML5; BD Biosciences), CD84 PE (CD84.1.21; BioLegend), CD38 PerCP-Cy5.5 (HIT2; BD Biosciences), CD95 BV421 (DX2; BioLegend), CD20 allophycocyanin-H7 (L27; BD Biosciences), CD27 BV605 (O323; BioLegend), CD3 VioGreen (BW264/56; Miltenyi Biotec), Ki67 FITC (B56; BD Biosciences), unconjugated goat anti-IRF4 (M-17; Santa Cruz Biotechnology), and donkey anti-goat IgG AF488 (polyclonal; Invitrogen). Controls were isotype-matched mouse mAbs. Annexin V FITC was from eBioscience, and 7-AAD was from BD Biosciences.
Reagents included human IL-2 (Roche), IL-6 (PeproTech), IFN-α (Sigma), IL-21 (PeproTech), goat anti-human F(ab′)2 fragments (anti-IgM and anti-IgG; Jackson ImmunoResearch), HybridoMax hybridoma growth supplement (Gentaur), Lipid Mixture 1, Chemically Defined (200×), and MEM Amino Acids Solution (50×, Sigma).
Reagents included human IL-2 (Roche), IL-6 (PeproTech), IFN-α (Sigma), IL-21 (PeproTech), goat anti-human F(ab′)2 fragments (anti-IgM and anti-IgG; Jackson ImmunoResearch), HybridoMax hybridoma growth supplement (Gentaur), Lipid Mixture 1, Chemically Defined (200×), and MEM Amino Acids Solution (50×, Sigma).
4
In Vitro Culture of Human Lymphocytes
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Reagents utilized in the in vitro culture system include human IL-2 (Roche); IFN-γ (Sigma); IL-6, IFN-α and IL-21 (PeproTech); goat anti-human F(ab’)2 fragments (anti-IgM and -IgG; Jackson Immunoresearch); HybridoMax hybridoma growth supplement (Gentaur); Lipid Mixture 1, chemically defined (200X) and MEM Amino Acids Solution (50X; Sigma); and PF-429242 (Tocris Bioscience).
The antibodies employed for protein detection include CREB3L2 (NBP1-88697, Novus Biologicals), ATF6 (40256; Novus Biological), ACTIN (A1978; Sigma), LC3A/B (Cell Signaling). The reagents utilized in flow cytometry assays include PE-conjugated anti-CD19 (LT19; Miltenyi Biotech), V450-conjugated anti-CD20 (2H7; E-Biosciences), PE-Cy7-conjugated anti-CD38 (HB7; BD Biosciences); APC-conjugated anti-CD138 (B-B4; Miltenyi Biotech); CountBright beads (Invitrogen) and 7-AAD (BD Biosciences).
The antibodies employed for protein detection include CREB3L2 (NBP1-88697, Novus Biologicals), ATF6 (40256; Novus Biological), ACTIN (A1978; Sigma), LC3A/B (Cell Signaling). The reagents utilized in flow cytometry assays include PE-conjugated anti-CD19 (LT19; Miltenyi Biotech), V450-conjugated anti-CD20 (2H7; E-Biosciences), PE-Cy7-conjugated anti-CD38 (HB7; BD Biosciences); APC-conjugated anti-CD138 (B-B4; Miltenyi Biotech); CountBright beads (Invitrogen) and 7-AAD (BD Biosciences).
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