Bx61vs microscope

Manufactured by Olympus

Sourced in Japan, Germany

The BX61VS is a research-grade microscope system designed for advanced imaging applications. It features a motorized, vertically-stable stand with precise positioning controls. The system is equipped with high-quality optics and illumination components to deliver clear, high-resolution images.

Automatically generated - may contain errors

Lab products found in correlation

31 protocols using bx61vs microscope

Aortic Root Lesion Quantification

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the study endpoint, hearts were removed from each mouse following PBS perfusion. After a brief PBS wash, individual mouse hearts were embedded in OCT and stored at −80°C until further use. About 8–10-micron serial sections of the aortic root were obtained by cutting the OCT-embedded hearts, as described earlier (27 (link)). Care was taken to ensure that only serial sections collected from aortic root regions representing about 100–150 microns following the valve leaflet were utilized in all morphometric experiments. Additional care was exercised to ensure that staining was performed on sections within similar regions of the aortic root across all treatment groups for quantification and comparison. Aortic root sections were concurrently stained with 0.5% w/v Oil red O (ORO) and hematoxylin and eosin (H&E) to assess the lipid burden and plaque area, respectively. Additional sections were utilized for the detection of collagen content within aortic root lesions using Masson’s Trichrome stain kit (Polysciences). For ORO-stained and MT-stained sections, hematoxylin counterstaining was utilized. All sections were mounted with DPX mounting media, observed using Olympus BX61VS microscope and images were captured using 10× magnification. For quantitative morphometry, eight animals per genotype for each sex with at least 30 sections within each group were analyzed.

Gupta S., Khanal S., Bhavnani N., Mathias A., Lallo J., Kiriakou A., Ferrell J, & Raman P. (2022). Sex-specific differences in atherosclerosis, thrombospondin-1, and smooth muscle cell differentiation in metabolic syndrome versus non-metabolic syndrome mice. Frontiers in Cardiovascular Medicine, 9, 1020006.

+ Open protocol

+ Expand

In situ Alu Sequence Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

In situ hybridization to detect human Alu repeat sequences was performed on paraffin‐embedded sections (N =2–3) using an ISH iVIEW Blue Plus Detection Kit (Ventana) and a BenchMark ULTRA automated IHC/ISH slide staining system (Ventana). According to the manufacturer's instructions, the Alu Positive Control Probe II was loaded with reagents from the detection kit and accessory reagents including Red Counterstain II onto the reagent tray and placed on the BenchMark ULTRA staining system. The slides were then loaded and the staining run was initiated. Human and rat tissues were used as positive and negative controls, respectively. After staining completed, the slides were dehydrated following the manufacturer's Dehydration Protocol. The slides were mounted with Surgipath MM 24 Mounting Medium (Leica Biosystems). All slides were imaged using an Olympus BX61VS microscope (Olympus Corporation, Tokyo, Japan, www.olympusamerica.com) with a Pike F‐505 camera (Allied Vision Technologies, Exton, PA, www.alliedvision.com).

Dang P.N., Herberg S., Varghai D., Riazi H., Varghai D., McMillan A., Awadallah A., Phillips L.M., Jeon O., Nguyen M.K., Dwivedi N., Yu X., Murphy W.L, & Alsberg E. (2017). Endochondral Ossification in Critical‐Sized Bone Defects via Readily Implantable Scaffold‐Free Stem Cell Constructs. Stem Cells Translational Medicine, 6(7), 1644-1659.

+ Open protocol

+ Expand

Alizarin Red S Staining of Cryosections

Check if the same lab product or an alternative is used in the 5 most similar protocols

After 4 weeks of culture, samples were embedded in OCT compound (Fisher Scientific) and frozen in liquid nitrogen. Ten micron thick sections were cut using a Microm HM 505E cryostat (Thermo Scientific, Waltham, MA), placed on Superfrost Plus microscope slides (Fisher Scientific) and kept frozen until staining. Before Alizarin Red S staining, slides were equilibrated to room temperature, fixed in acetone for 20 min and then stained with 2% Alizarin Red S (pH = 4.15, Sigma Aldrich) for 5 min. Slides were rinsed with acetone and mounted with Permount mounting medium (Fisher Scientific). Photomicrographs were acquired with an Olympus BX61VS microscope (Olympus, Center Valley, PA) with a Pike F-505 camera (Allied Vision Technologies, Stadtroda, Germany). N = 4 samples from each condition were sectioned and stained, with representative images shown.

Samorezov J.E., Headley E.B., Everett C.R, & Alsberg E. (2016). Sustained presentation of BMP-2 enhances osteogenic differentiation of human adipose-derived stem cells in gelatin hydrogels. Journal of biomedical materials research. Part A, 104(6), 1387-1397.

+ Open protocol

+ Expand

Quantifying Neurogenesis and Angiogenesis in Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed on 40 μm thick brain coronal sections using anti-DCX (1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-CD31 (1:100; BD) to measure neurogenesis and vessel density respectively. Positive cells were stained by reaction with 3,3′-diaminobenzidine (Vector Laboratories, Burlingame, CA, USA) as previously described^{33 (link), 75 (link)}. For each reaction, adequate negative controls were performed. DCX and CD31 stainings were acquired at 20x of magnification by Olympus BX-61-VS microscope. Images were analyzed using software Fiji.The newly generated neuroblasts were quantified as percentage of DCX stained area (green box, Fig. 5A). The vessel density was quantified by overlaying digitalized images with a grid (10 × 10 μm per single square). The vascular network was quantified in lateral (red box, Fig. 4B) and medial (blue box, Fig. 4B) cortex, by counting the number of vessels crossing the grid and normalizing the values over the area analyzed. Data are expressed as the percentage of vessel density in the ipsilateral over contralateral hemisphere.

Sammali E., Alia C., Vegliante G., Colombo V., Giordano N., Pischiutta F., Boncoraglio G.B., Barilani M., Lazzari L., Caleo M., De Simoni M.G., Gaipa G., Citerio G, & Zanier E.R. (2017). Intravenous infusion of human bone marrow mesenchymal stromal cells promotes functional recovery and neuroplasticity after ischemic stroke in mice. Scientific Reports, 7, 6962.

+ Open protocol

+ Expand

Tumor Angiogenesis Immunohistochemical Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Collected tumor tissue and Matrigel plugs were embedded in OCT (Tissue-Tek) and cryosectioned at 7–10 µM thickness on coated slides. Sections from both the central and peripheral regions of the tumors were used for staining. Frozen sections were stained with the following primary antibodies were added to the sections: CD31 (1:50) (Invitrogen), NG2 (1:200) (Millipore), and VE-cadherin (1:100) (Santa Cruz) and secondary antibody conjugated with Alexa-Fluor 488 or Alexa-Fluor 594 (Invitrogen) as described previously [1 (link), 26 (link)–27]. Lung tissue was processed and embedded in paraffin and cut using a Leica microtome (Leica Biosystems) at 7–10 µM thickness on coated slides. Prior to staining, sections were deparaffinized in a 60°C oven and rehydrated in xylene and varying percentages of alcohol and then stained for H&E. Slides were then dehydrated in varying percentages of alcohol and xylene followed by mounting with DPX Mountant (Sigma Aldrich). Images were captured using either Olympus IX81 or Olympus BX61VS Microscope and processed using NIH Image J software.

Cappelli H.C., Kanugula A.K., Adapala R.K., Amin V., Sharma P., Midha P., Paruchuri S, & Thodeti C.K. (2018). Mechanosensitive TRPV4 channels stabilize VE-cadherin junctions to regulate tumor vascular integrity and metastasis. Cancer letters, 442, 15-20.

+ Open protocol

+ Expand

Aortic Root Plaque Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the study endpoint, mouse hearts were removed after PBS perfusion. After a brief PBS wash, individual hearts were OCT-embedded and stored at −80 °C for further processing. Following dissection of the OCT-embedded hearts, serial sections (8 microns) of the aortic root were obtained, as reported earlier [77 (link)]. For all morphometric measurements, serial sections within 100–150 microns from the valve leaflet were utilized. Moreover, care was taken to ensure that only sections from comparable areas of the aortic root across all treatment groups were included for further staining and quantification. Concurrent aortic root sections derived from each mouse were stained with 0.5% Hematoxylin and Eosin (H & E) and Oil red O (ORO) solutions to detect plaque area and lipid burden, respectively. Furthermore, hematoxylin was used as a counterstain for all ORO-stained sections. Tissue sections were mounted using the DPX mounting media (Electron Microscopy Sciences) and images were captured at 10X magnification using the Olympus BX61VS microscope. For quantitative morphometry, we utilized a total of 7 mice for each genotype, and at least 30 sections from each group were analyzed.

Khanal S., Bhavnani N., Mathias A., Lallo J., Gupta S., Ohanyan V., Ferrell J.M, & Raman P. (2023). Deletion of Smooth Muscle O-GlcNAc Transferase Prevents Development of Atherosclerosis in Western Diet-Fed Hyperglycemic ApoE-/- Mice In Vivo. International Journal of Molecular Sciences, 24(9), 7899.

+ Open protocol

+ Expand

Histological Analysis of Cartilage Rings

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue rings and 3-ring tubes (N≥2) were fixed in 10% neutral buffered formalin overnight, embedded in paraffin and sectioned at 5 microns. Rings were sectioned in either axial or vertical planes. Tubes were sectioned first in the axial plane and then re-embedded in paraffin and sectioned in the vertical plane. Mounted tissue sections were deparaffinized and rehydrated. Safranin O (Acros Organics) was used to stain for sulfated GAG content with a Fast Green counterstain (Fisher Chemical). For immunohistochemical staining, the presence of type II collagen was detected using anti-collagen type II primary antibody (abcam ab34712, Cambridge, UK) with a Fast Green counterstain. A section of the human knee articular cartilage and underlying subchondral bone served as a positive and negative control, respectively. Samples stained with isotype-matched IgG instead of primary antibody also served as negative controls. Histostain-Plus Bulk kit (Invitrogen) with aminoethyl carbazole (AEC; Invitrogen) was used to visualize the primary antibody. Images of stained tissues were acquired using an Olympus BX61VS microscope (Olympus, Center Valley, PA) with a Pike F-505 camera (Allied Vision Technologies, Stadtroda, Germany).

Dikina A.D., Strobel H.A., Lai B.P., Rolle M.W, & Alsberg E. (2015). Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs. Biomaterials, 52, 452-462.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Breast Cancer Samples

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The research involved the analysis of human specimens through the utilization of tissue microarray (TMA) slides procured from SuperBioChips Laboratories in Seoul, Republic of Korea. These TMA slides encompassed samples from human breast cancer, metastatic cancer, and normal tissues. The immunohistochemistry (IHC) on the TMA slides was conducted following the established protocol outlined in a prior study [5 (link), 29 (link)]. The IHC protocol entailed the use of specific antibodies to label and detect the proteins of interest within the tissue samples. Initial steps included fixation of samples with paraformaldehyde (4%) and permeabilization with Triton X-100 (0.2%). Subsequently, non-specific antibodies were blocked using BSA (5%) before the application of specific antibodies. Following an overnight incubation with primary antibodies at 4 °C, secondary antibodies were applied and left to incubate for 30 min at room temperature. The slides underwent examination and analysis at Li-Tzung Pathology Laboratory in Kaohsiung, Taiwan, with whole slide images captured using a BX61VS^® microscope manufactured by Olympus in Tokyo, Japan.

Li C.J., Tzeng Y.D., Hsiao J.H., Tseng L.M., Hsu T.S, & Chu P.Y. (2024). Spatial and single-cell explorations uncover prognostic significance and immunological functions of mitochondrial calcium uniporter in breast cancer. Cancer Cell International, 24, 140.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Integrin β-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lung sections were de-paraffinized, subjected to boiling citrate buffer (pH = 6.0) for antigen retrieval, and blocked with 10% normal goat serum in PBS for 2 hr at room temperature. The primary antibody, mouse anti-integrin β−1/CD29 (MAB17783), was added and incubated for 12 h at 4 °C, or 4 hr at room temperature at a 1:100 dilution. For all stains, the secondary antibody, goat anti-mouse IgG 647 (ab150116), was diluted 1:200 and incubated for 1 hr at room temperature. Sections were mounted in Vectashield Mounting Medium with DAPI (Vector Laboratories), coverslipped, and imaged using an Olympus FSX100 or BX61VS microscope (Olympus).

Kim J., Guenthart B., O’Neill J.D., Dorrello N.V., Bacchetta M, & Vunjak-Novakovic G. (2017). Controlled delivery and minimally invasive imaging of stem cells in the lung. Scientific Reports, 7, 13082.

+ Open protocol

+ Expand

Measuring Frozen Aggregate Diameters

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen aggregates (n = 4 per group; donor B) were thawed in PBS and imaged using an Olympus BX61VS microscope (Olympus, Center Valley, PA, http://www.olympusamerica.com) equipped with a Pike F-505 camera (Allied Vision Technologies, Stadtroda, Germany, https://www.alliedvision.com). The diameter of each aggregate was obtained by averaging measurements at 3 different positions (12 to 6, 2 to 8, and 4 to 10 o’clock) using ImageJ software (NIH, Washington, DC, http://www.nih.gov).

Dang P.N., Dwivedi N., Phillips L.M., Yu X., Herberg S., Bowerman C., Solorio L.D., Murphy W.L, & Alsberg E. (2015). Controlled Dual Growth Factor Delivery From Microparticles Incorporated Within Human Bone Marrow-Derived Mesenchymal Stem Cell Aggregates for Enhanced Bone Tissue Engineering via Endochondral Ossification. Stem Cells Translational Medicine, 5(2), 206-217.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!