B6.129 sirt2tm1 1fwa j

All the procedures involving animal use were in compliance with the policies of the Institutional Animal Care and Use Committee (IACUC) at Boston University (protocol #: 14-033). C57BL/6J wild-type (#000664) and SIRT2 knock-out mice (B6.129-Sirt2^tm1.1Fwa/J, #012772) were obtained from Jackson Laboratory (Bar Harbor, ME). Mouse colonies were maintained in the Laboratory Animal Care Facility (LACF) at Boston University, Charles River Campus. The mice aged 8–15 weeks of either sex were randomly selected from different litters. Only mice with normal body size, morphology and no observable disabilities were used for the experiments.

C57BL/6J mice, Pmel mice with gp100-reactive TCR:
B6.Cg-Thy1^a/Cy Tg(TcraTcrb)8Rest/J, OT-II
mice with MHC II-restricted OVA-specific TCR: B6.Cg-Tg(TcraTcrb)425Cbn/J,
Sirt2^−/− mice:
B6.129-Sirt2^tm1.1Fwa/J and NSG mice:
NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ were purchased from The Jackson
Laboratory. Pmel mice and OT-II mice were crossed with
Sirt2^−/− mice to generate
Sirt2^−/− Pmel mice and
Sirt2^−/− OT-II mice,
respectively. All mice were bred and maintained under specific pathogen-free
conditions at the animal facility of H. Lee Moffitt Cancer Center. All
animal protocols were approved by the Institutional Animal Care and Use
Committee (IACUC). Mice were used at 7–8 weeks of age with age- and
sex-matched controls.