Ft ir 240 c spectrophotometer

All the chemicals were purchased from Sigma-Aldrich, and all the solvents for the use were of laboratory grade. The materials for in vitro evaluation were obtained from Sigma-Aldrich Ltd. The reactions were monitored by thin layer chromatography (TLC) on precoated silica gel 60 F254 (Merck, Darmstadt, Germany), and spots were visualized with UV light. Merck silica gel (80–120 mesh; Merck, Darmstadt, Germany) was used for column chromatography. All the synthesized compounds were purified by recrystallization and column chromatography. Melting points were determined via an open capillary method and were uncorrected. IR spectra were recorded on a Perkin-Elmer FT-IR 240-C Spectrophotometer using KBr optics (Perkin-Elmer, Waltham, MA, USA). Next, 1H-NMR spectra were recorded on Gemini Varian (Varian, Palo Alto, CA, USA) 200 MHz, Bruker (Bruker Bioscience, Billerica, MA, USA) AV 300 MHz, and Unity (Varian) 400 MHz spectrometer in DMSO-d6 or CDCl3 using TMS as an internal standard. Electron impact (EI) and chemical ionization mass spectra were recorded on a VG 7070 H instrument (Micromass, St Leonards-0n Sea, UK) at 70 eV.