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Pfm450e

Manufactured by Thorlabs
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The PFM450E is a power and energy meter from Thorlabs. It is designed to measure optical power and energy across a wide range of wavelengths. The device features a high-sensitivity detector and provides accurate measurements with a wide dynamic range.

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Lab products found in correlation

Gaasp photomultiplier tube, by Hamamatsu Photonics (2 mentions) Lsk gr08, by Thorlabs (2 mentions) Thorimage 3, by Thorlabs (2 mentions) 0.8 numerical aperture microscope objective, by Nikon (2 mentions) Mai tai hp, by Spectra-Physics (2 mentions) Matlab 2021a, by MathWorks (1 mentions) Deepsee, by Spectra-Physics (1 mentions)

3 protocols using pfm450e

1

Two-Photon Imaging of Drosophila Neural Circuits

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We used a two-photon microscope equipped with a galvo-galvo-resonant scanhead (Thorlabs Bergamo II GGR) and ×25, 1.10 numerical aperture (NA) objective (Nikon CFI APO LWD; Thorlabs, WDN25X-APO-MP). For volumetric imaging, we used a fast piezoelectric objective scanner (Thorlabs PFM450E). To excite GCaMP we used a wavelength-tunable femtosecond laser with dispersion compensation (Mai Tai DeepSee, Spectra Physics) set to 920 nm. GCaMP fluorescence signals were collected using GaAsP PMTs (PMT2100, Thorlabs) through a 405–488 nm band-pass filter (Thorlabs). All image acquisition and microscope control was conducted in MATLAB 2021a (MathWorks Inc), using ScanImage 2021 Premium with vDAQ hardware (Vidrio Technologies LLC) and custom MATLAB scripts for further experimental control. The region for imaging the fan-shaped body and protocerebral bridge was 150 × 250 pixels, whereas the region for imaging the LAL was 150 × 400 pixels. We acquired 10–12 slices in the z axis for each volume (4 µm per slice), resulting in 6–8 Hz volumetric scanning rate. For experiments using the selective PFL2 split-Gal4 line, we imaged in the protocerebral bridge, fan-shaped body, or LAL for different trials. For experiments imaging the mixed PFL2 and PFL3 split-Gal4 line, we only imaged in the LAL.
Westeinde E.A., Kellogg E., Dawson P.M., Lu J., Hamburg L., Midler B., Druckmann S, & Wilson R.I. (2024). Transforming a head direction signal into a goal-oriented steering command. Nature, 626(8000), 819-826.
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2

Two-Photon Calcium Imaging of Neural Activity

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Two-photon calcium imaging was performed on a custom-build microscope equipped with a resonant scanning module (LSK-GR08, Thorlabs), GaAsP photomultiplier tube (Hamamatsu), and a 16 × 0.8 numerical aperture microscope objective (Nikon) using ThorImage 3.1 (Thorlabs). We used a Ti-Sapphire laser (Mai Tai HP, Spectra Physics) to excite GCaMP6 at 920 nm. The FOV was 798 μm × 798 μm (512 × 512 pixels) and images were acquired at 30 Hz for single-plane recordings and at 5 Hz for multi-plane recordings. For multi-plane recordings the objective was moved between frames using a piezo objective scanner (PFM450E, Thorlabs). The piezo was allowed to settle for 35 ms in the new z position before the next frame was recorded.
Buetfering C., Zhang Z., Pitsiani M., Smallridge J., Boven E., McElligott S, & Häusser M. (2022). Behaviorally relevant decision coding in primary somatosensory cortex neurons. Nature neuroscience, 25(9), 1225-1236.
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3

Two-Photon Calcium Imaging of Neural Activity

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Two-photon calcium imaging was performed on a custom-build microscope equipped with a resonant scanning module (LSK-GR08, Thorlabs), GaAsP photomultiplier tube (Hamamatsu), and a 16 × 0.8 numerical aperture microscope objective (Nikon) using ThorImage 3.1 (Thorlabs). We used a Ti-Sapphire laser (Mai Tai HP, Spectra Physics) to excite GCaMP6 at 920 nm. The FOV was 798 μm × 798 μm (512 × 512 pixels) and images were acquired at 30 Hz for single-plane recordings and at 5 Hz for multi-plane recordings. For multi-plane recordings the objective was moved between frames using a piezo objective scanner (PFM450E, Thorlabs). The piezo was allowed to settle for 35 ms in the new z position before the next frame was recorded.
Buetfering C., Zhang Z., Pitsiani M., Smallridge J., Boven E., McElligott S, & Häusser M. (2022). Behaviorally relevant decision coding in primary somatosensory cortex neurons. Nature neuroscience, 25(9), 1225-1236.
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