EPCs (5 × 104 cells) were seeded onto transwells (6.5 mm, 8 μm pore; BD Biosciences) in IMDM supplemented with 0.5% FBS. IMDM/0.5% FBS with recombinant human SDF-1 (200 ng/mL; R&D Systems) was added to the lower chamber. The assays were performed under different oxygen tensions (20% or 5%) for 6 h, and nonmigrated cells were wiped away from the top surface of the membrane. Cells that adhered to the undersurface of the membrane were stained with Diff-Quik staining solution (International Reagents) and then counted using an inverted microscope.
Recombinant human sdf 1
Manufactured by R&D Systems
Recombinant human SDF-1 is a protein produced using recombinant DNA technology. It is a chemokine that plays a role in the regulation of cell migration and homing.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant human sdf 1
1
Endothelial Progenitor Cell Migration
2
Fibroblast and Endothelial Cell Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro experiments were conducted in NRK-49F renal fibroblasts (ATCC, Manassas, VA) and human umbilical vein endothelial cells (HUVECs) [4] (link), [23] (link). NRK-49F cells were treated with 10 ng/ml recombinant rat transforming growth factor β (TGF-β) (R&D Systems) for 24 h, with or without pre-treatment with the histone deacetylase (HDAC) inhibitor vorinostat (5 μM) (Exclusive Chemistry, Obninsk, Russia) for 4 h. HUVECs were pre-incubated with 20 μM LY294002 (LC Laboratories, Woburn, MA), 1 μM AMD3100 or vehicle (0.1% DMSO) for 30 min before the addition of recombinant human SDF-1 (100 ng/ml) (R&D Systems) (or 1% BSA) for 30 min.
+ Expand
In vitro experiments were conducted in NRK-49F renal fibroblasts (ATCC, Manassas, VA) and human umbilical vein endothelial cells (HUVECs) [4] (link), [23] (link). NRK-49F cells were treated with 10 ng/ml recombinant rat transforming growth factor β (TGF-β) (R&D Systems) for 24 h, with or without pre-treatment with the histone deacetylase (HDAC) inhibitor vorinostat (5 μM) (Exclusive Chemistry, Obninsk, Russia) for 4 h. HUVECs were pre-incubated with 20 μM LY294002 (LC Laboratories, Woburn, MA), 1 μM AMD3100 or vehicle (0.1% DMSO) for 30 min before the addition of recombinant human SDF-1 (100 ng/ml) (R&D Systems) (or 1% BSA) for 30 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!