Deltavision core microscope system

Manufactured by GE Healthcare

The Deltavision Core microscope system is a high-performance imaging platform designed for advanced microscopy applications. It features a modular design, allowing for customization to suit various research needs. The system provides precise control and exceptional image quality, enabling detailed analysis of cellular and subcellular structures.

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Lab products found in correlation

1.42 na oil immersion objective, by Olympus (1 mentions) Glass bottom culture dishes, by MatTek (1 mentions) Softworx, by Cytiva (1 mentions)

Close spelling variants of this product

DeltaVision microscope (58 citations) DeltaVision system (15 citations) DeltaVision microscope system (14 citations) DeltaVision Core microscope (8 citations) DeltaVision Core system (6 citations)

2 protocols using deltavision core microscope system

Time-Lapse Imaging of Neural Tube Explants

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Time-lapse imaging of neural tube explants was performed using a Deltavision Core microscope system in a WeatherStation environmental chamber maintained at 37°C (GE Healthcare). Imaging was optimised for minimal light transmission, exposure times (50–100 ms) and total acquisition times (10–20 min) to avoid phototoxicity (Das et al., 2012 (link); Das and Storey, 2014 (link)). Image acquisition was performed using an Olympus 60×1.42 NA oil immersion objective or an Olympus 40×1.25 NA silicone oil immersion objective, a solid-state LED light source and a CoolSnap HQ2 cooled CCD camera (Photometrics). Unless otherwise stated, 10 optical sections spaced 0.5 μm apart were acquired for each explant (1024×1024 pixels, 1×1 binning). For the live-imaging sessions totalling for 1 h, image acquisition between timepoints was set at 1-min intervals. Images were deconvolved using the SoftWorx image processing software.

Kasioulis I., Dady A., James J., Prescott A., Halley P.A, & Storey K.G. (2022). A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis. Journal of Cell Science, 135(6), jcs259897.

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Microscopic Analysis of Sporulating Cells

Cited 1 time

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Cells were induced to sporulate by resuspension in SM medium (Sterlini and Mandelstam, 1969 (link)). For microscopy, cells were harvested from 1 ml culture by centrifugation and resuspended in 100 μl SM medium. If required, the membrane dye FM4–64 was added at 15 μg/ml and incubated for at least 15 min at room temperature. 3 μl cell suspension were placed on glass-bottom culture dishes (Mattek Corp). A pad made of 1% agarose (in ultrapure water) was cut to size and placed over the cells. Cells were viewed at room temperature with a DeltaVision Core microscope system (GE Healthcare/Applied Precision) equipped with a Photometrics CoolSnap HQ2 camera as described previously (Eswaramoorthy et al., 2014 (link)). Ten z-planes were acquired at 200-nm intervals and the data were deconvolved using SoftWorx (ver. 5.5, Applied Precision/GE Healthcare). Analysis of fluorescence intensities along line scans was performed using ImageJ. Images shown are representatives of three independent experiments.

Kim E.Y., Tyndall E.R., Huang K.C., Tian F, & Ramamurthi K.S. (2017). Dash-and-recruit mechanism drives membrane curvature recognition by the small bacterial protein SpoVM. Cell systems, 5(5), 518-526.e3.

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