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Living image software

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Living Image software is a powerful imaging platform designed for scientific and research applications. It provides a comprehensive suite of tools for image acquisition, analysis, and visualization. The software's core function is to enable users to capture, process, and interpret high-quality images from a variety of imaging devices, supporting a wide range of file formats and experimental setups.

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Lab products found in correlation

D luciferin firefly potassium salt, by Biosynth (2 mentions) Ami ht, by Spectral Instruments Imaging (1 mentions)

3 protocols using living image software

1

In vivo Cell Tracking in MI Mice

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MI was performed as described previously. To track the cell grafts in vivo, iECs/iCMs (3 × 105; iCMs and iECs at a ratio of 2:1) were incubated with DiR (AAT Bioquest, USA) according to the manufacturer's instructions. MI model mice were randomly divided into 2 groups as follows (n = 4 in each group): (1) DiR-iECs/iCMs and (2) DiR-iECs/iCMs encapsulated in SpGel. A total of 1 × 105 iECs and 2 × 105 iCMs were resuspended in 20 μl SpGel or PBS and intramyocardially injected into the hearts. On days 0 and 7, the mice were imaged under general anesthesia with 1.0% inhaled isoflurane using the IVIS 200 system (Xenogen). The maximum signal from an ROI was measured using Living Image software (Media Cybernetics).
Guan G., Huo D., Li Y., Zhao X., Li Y., Qin Z., Sun D., Yang G., Yang M., Tan J., Zeng W, & Zhu C. (2021). Engineering hiPSC-CM and hiPSC-EC laden 3D nanofibrous splenic hydrogel for improving cardiac function through revascularization and remuscularization in infarcted heart. Bioactive Materials, 6(12), 4415-4429.
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2

Bioluminescence Imaging of Mice

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For BLI, D-luciferin firefly potassium salt (375 mg kg−1; Biosynth) was dissolved in PBS (pH 7.4) (Gibco, Invitrogen) and was injected intraperitoneally (250 μl per mouse) into anesthetized mice. Animals were imaged using the IVIS 200 system (Xenogen). Region of interest (ROI) bioluminescence was quantified in units of maximum photons per second per centimeter square per steradian (p s−1 cm−2 sr−1). The maximum signal from an ROI was measured using Living Image software (MediaCybernetics). Mice were monitored on day 0, day 1 and every other day until day 30 and every 10 days afterwards.
Deuse T., Hu X., Gravina A., Wang D., Tediashvili G., De C., Thayer W.O., Wahl A., Garcia J.V., Reichenspurner H., Davis M.M., Lanier L.L, & Schrepfer S. (2019). Hypoimmunogenic derivatives of induced pluripotent stem cells evade immune rejection in fully immunocompetent allogeneic recipients. Nature biotechnology, 37(3), 252-258.
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3

Bioluminescence Imaging of Humanized Mice

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For BLI, D-luciferin firefly potassium salt (375 mg kg−1) (Biosynth) dissolved in sterile PBS (pH 7.4) (Gibco, Invitrogen) was injected intraperitoneally (250 μl per mouse) into anesthetized mice. Animals were imaged using the ami HT (Spectral Instruments Imaging) ROI bioluminescence was quantified in units of maximum photons per second per centimeter square per steradian (p s−1 cm−2 sr−1). The maximum signal from an ROI was measured using Living Image software (MediaCybernetics). Humanized mice were injected with 5 × 105 or 1 × 106 cells in pro survival scaffold as described above. Mice were monitored on day 0, day 1 and every 4 days until cells were rejected or up to 50 days.
Deuse T., Hu X., Gravina A., Wang D., Tediashvili G., De C., Thayer W.O., Wahl A., Garcia J.V., Reichenspurner H., Davis M.M., Lanier L.L, & Schrepfer S. (2019). Hypoimmunogenic derivatives of induced pluripotent stem cells evade immune rejection in fully immunocompetent allogeneic recipients. Nature biotechnology, 37(3), 252-258.
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