ZO-1 and F4/80 staining of the colon was detected by staining the colonic tissue sections (5 μm) with anti-ZO-1 antibody (Servicebio, GB11195, 1:200 dilution) in PBS, anti-F4/80 antibody (Servicebio, GB11027, 1:1000 dilution) in PBS and goat-anti-rabbit CY3 conjugated antibody (Servicebio, GB21303, 1:300 dilution) in PBS. Finally, the sections were counterstained with DAPI (Servicebio, G1012). At a temperature of − 18°C, 20 μm frozen brain sections were cut using a cryostat. The brain slices were blocked with 3% bovine serum albumin for 30 min at room temperature and then incubated with the primary antibodies at 4°C overnight. The primary antibody anti-Iba1 (Servicebio, GB13105, 1:500 dilution) and anti-GFAP (Servicebio, GB11096, 1:800 dilution) were used. After washing with PBS, the sections were incubated with the secondary antibodies at 37°C for 50 min. The secondary antibody goat-anti-rabbit Cy3 conjugated antibody (Servicebio, GB21303, 1:300 dilution) were used. Finally, the sections were counterstained with DAPI (Servicebio, G1012) and then imaged with microscope (Nikon Eclipse C1). Quantification of positively stained cells in the PFC and HIP regions were used ImageJ.
G1012
Manufactured by Nikon
Sourced in Japan
The G1012 is a laboratory instrument manufactured by Nikon. It is designed to perform precise measurements and analysis of various samples. The core function of the G1012 is to provide accurate and reliable data for research and testing purposes.
Automatically generated - may contain errors
Lab products found in correlation
Gb21303, by Wuhan Servicebio Technology (2 mentions)
Gb11096, by Wuhan Servicebio Technology (1 mentions)
Gb11027, by Wuhan Servicebio Technology (1 mentions)
Gb11195, by Wuhan Servicebio Technology (1 mentions)
Eclipse c1, by Nikon (1 mentions)
Gb13105, by Wuhan Servicebio Technology (1 mentions)
G1012, by Wuhan Servicebio Technology (1 mentions)
Ab3611, by Abcam (1 mentions)
A1r confocal microscope, by Nikon (1 mentions)
2 protocols using g1012
1
Immunohistochemical Analysis of Colon and Brain
2
Immunohistochemical Analysis of Neural Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were anesthetized with an intraperitoneal injection of 4% chloral hydrate (350 mg/kg) and transcardially perfused with a short prerinse of 20-40 ml of ice-cold physiological saline, followed by 20-40 ml of 4% paraformaldehyde (PFA). Whole tissues were then post xed in 4% PFA at 4°C overnight. After dehydration with 15% and 30% sucrose for 48 h, coronal 30-μm sections were cut using a sliding freezing microtome. The slices were incubated at room temperature for 100 minutes in goat serum, followed by a 24 h incubation with the following primary antibodies: anti-doublecortin (DCX, Abcam, ab18723, 1:500), anti-HMGB1 (GeneTex, GTX101277, 1:600), and anti-RAGE (Abcam, ab3611, 1:300). Proteins were visualized by detection with CY3 secondary antibodies (Servicebio, GB21303, 1:400) after adding the secondary antibody after 1 h at room temperature. The slices were mounted with DAPI (Servicebio, G1012) before images were captured with a Nikon A1R confocal microscope (Nikon, Japan). The uorescence data were analyzed using ImageJ software.
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