Nucleosil si 300 silica

The HSA (essentially fatty acid free, ≥ 96% pure), Con A (type V, lyophilized powder, highly purified), glycogen (bovine liver type IX, total glucose ≥ 85 %, dry basis), racemic warfarin (purity ≥ 98%), 4-methylumbellipheryl α-D-mannopyranoside (MUM, purity ≥ 96%) and 4-methylumbellipheryl α-D-galactopyranoside (MUGA, purity ≥ 98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nucleosil Si-300 silica (7 μm particle diameter, 300 Å pore size) was obtained from Macherey Nagel (Duren, Germany). All other chemicals were of the purest grades available. Vivaspin 6 ultrafiltration tubes (30 kDa cutoff; Sartorius, Gottingen, Germany) were used for purification of the oxidized glycogen. All buffers and aqueous solutions were prepared using water from a Nanopure system (Barnstead, Dubuque, IA, USA). Buffers were filtered using 0.20 μm GNWP nylon membranes from Fisher Scientific (Pittsburgh, PA, USA) and were degassed by sonication under vacuum for at least 30 min prior to use.

The AGP (from pooled human plasma, product no. G9885, ≥ 99% pure), L-fucose (> 99%), (3-glycidoxypropyl)trimethoxysilane (≥ 98%), Con A (product C7275, type V, highly purified), human serum albumin (HSA, product A1887, 96%, fatty acid free), ovalbumin (≥ 98%), p-nitrophenyl α-D-mannopyranoside (p-NP-α-D-Man) and p-nitrophenyl α-L-fucopyranoside (p-NP-α-L-Fuc) were purchased from Sigma-Aldrich (St Louis, MO, USA). The AAL (product L-1390, homogeneous by SDS-PAGE) was purchased from Vector Laboratories (Burlingame, CA, USA). Nucleosil Si-300 silica (300 Å pore size, 5 μm particle diameter) was purchased from Macherey-Nagel (Duren, Germany). A micro BCA assay kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA). All aqueous solutions and samples were prepared using water from a Milli-Q Advantage A10 Water Purification System (EMD Millipore Corporation, Billerica, MA, USA).

The glibenclamide (≥ 99% pure), glimepiride (≥ 99%), glipizide (≥ 96%), and HSA (Cohn fraction V, essentially fatty acid free, ≥ 96%) were from Sigma (St. Louis, MO, USA). Nucleosil Si-300 silica (7 μm particle diameter, 300 Å pore size) was acquired from Macherey Nagel (Dűren, Germany). The components for the bicinchoninic acid (BCA) protein assay were purchased from Pierce (Rockford, IL, USA). All aqueous solutions and buffers were prepared using water that was generated by a NANOpure system (Barnstead, Dubuque, IA, USA) and were passed through Osmonics 0.22 μM nylon filters from Fisher Scientific (Pittsburgh, PA, USA).