Inveon micro ct pet scanner

Micro-CT of the hind limbs was performed using a Siemens Inveon Micro-CT/PET scanner (Siemens Medical Solutions, Knoxville, TN, USA).

Mouse skulls from three experimental and control animals were scanned with a Siemens Inveon Micro-CT/PET scanner using 60 kVp and 500 mA with a voxel size of 30 μm. Reconstructed images were imported using Osirx DICOM software. Mouse heads were prepared by overnight fixation at 4°C, followed by storage in 70% EtOH. Scans directed across the anterior-posterior plane produced 2D images which were matched between animals using topology markers such as the molar.

To analyze variation in gross craniomandibular dimensions, we imaged n = 3 WT and n = 2 Fox06^-/- mice using a Siemens Inveon Micro-CT/PET scanner. Skulls were scanned at 60kVp and 500mA with a voxel size of 30μm and reconstructed images were imported into Osirx DICOM imaging software [165 (link)] for morphometric analysis. Using two- and three- dimensional renderings we collected a series of linear anterior-posterior and transverse skeletal and dental measurements defined in Fig 3. Hemimandibles with soft tissues removed were scanned in a μCT-40 (Scanco, Brüttisellen, Switzerland) at 70 kV, 114 mA, and 6 μm resolution. Images were processed with μCT-40 evaluation software and FIJI (https://fiji.sc/) was used to orient the mandibles in a standardized way based on anatomical landmarks to clearly observe and compare enamel mineralization in two planes 1) through the first molar, in a coronal plane through the distal root and, extending this plane, in the early maturation stage of the developing incisor and, 2) in the maturation stage incisor, in coronal plane through the mandible.