We used a Leica confocal microscope TCS SP5 II (objective 63-fold) and examined the images with Leica LAS-AV software. Investigated cells were stained using the wheat germ agglutinin (WGA) Alexa Fluor® 488 conjugate (4 µg/ml, W11261, Life Technologies GmbH, Germany) according to the manufacturer's instructions. The following laser power was used for Cy7, DAPI and WGA Alexa Fluor® 488 conjugate: 643-800 nm, 415-466 nm and 498-547 nm, respectively.
Las av software
Manufactured by Leica
LAS-AV software is a comprehensive imaging and analysis platform designed for Leica microscopes. It provides a range of tools for image acquisition, processing, and analysis, enabling researchers to effectively manage and analyze their microscopy data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using las av software
1
Confocal imaging of cellular structures
2
Visualizing Milk and Yogurt Microstructure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Leica confocal laser scanning microscope (Model TCS-SP5, Leica Microsystems, Wetzlar, Germany) was used to visualize the microstructure of milk and yogurt samples. First, 1 mL of each sample was mixed with 100 µL of Nile Red (0.02% w/w in 1,2-propanediol) and 100 µL of Fast Green (0.1% w/w in water) and incubated at room temperature for 30 min. The stained samples were then placed onto the glass slides, and dual excitation at 488 nm (argon laser) and 633 nm (HeNe 633 laser) was employed (emission wavelength was 563-610 nm for Nile Red and 650-705 nm for Fast Green). Confocal micrographs were obtained using a 63x oil immersion objective with 1.4 numerical aperture. Digital images of 1024×1024 pixels were recorded by Leica LAS AV software (V 2.7.3.9723).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!