The effects of YAP/Mask2 complex on cell growth were determined by Cell Counting Kit-8 assay (Transgene, China). Briefly, 5×103 cells per well were seeded in a 96-well plate for 12 hours and then transfected as mentioned above. After 48 hours of transfection, 100μl fresh medium containing 10% of CCK-8 was replaced to each well and the cells were cultured for another 1 hour. The absorbance at 450 nm was determined using an ELISA microplate reader (Bio-Rad). Experiments were repeated for three times.
Cell counting kit 8 (cck8)
Manufactured by Transgene
Sourced in China
The Cell Counting Kit-8 is a colorimetric assay used for determining the number of viable cells in a cell proliferation or cytotoxicity assay. It utilizes a water-soluble tetrazolium salt to measure the activity of dehydrogenases in living cells, providing a quantitative assessment of cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Elisa microplate reader, by Bio-Rad (3 mentions)
Microplate reader, by Bio-Rad (3 mentions)
Cck 8, by Transgene (3 mentions)
Microplate reader, by Agilent Technologies (2 mentions)
Edu apollo dna in vitro kit, by RiboBio (2 mentions)
Multiskan go, by Thermo Fisher Scientific (2 mentions)
Synergy 2, by Agilent Technologies (1 mentions)
Prism 9, by GraphPad (1 mentions)
Beyoclick edu cell proliferation kit, by Beyotime (1 mentions)
Spectramax m2e, by Molecular Devices (1 mentions)
Transdetect annexin 5 egfp pi cell apoptosis detection kit, by Transgene (1 mentions)
Imark microplate reader, by Bio-Rad (1 mentions)
Automatic microplate reader, by Bio-Rad (1 mentions)
5 ethynyl 20 deoxyuridine edu assay kit, by RiboBio (1 mentions)
Cck 8, by Bio-Rad (1 mentions)
Multi mode microplate reader, by Molecular Devices (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Edu assay kit, by RiboBio (1 mentions)
Spectra max i3x multifunctional microplate detection system, by Molecular Devices (1 mentions)
Irinotecan, by Merck Group (1 mentions)
30 protocols using cell counting kit 8 (cck8)
1
Cell Growth Analysis by CCK-8 Assay
2
Evaluating Cytotoxicity of SR in MC3T3-E1 Cells
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The cytotoxicity of SR was determined using Cell Counting Kit-8 (CCK-8, Transgen Biotech, Beijing, China). According to the manufacturer’s protocol, MC3T3-E1 cells were harvested and adjusted to the density at 1.0 × 105/ml, inoculating into a 96-well cell culture plate at 200 μl per well. After cell adherence, different concentrations (0.1–5.0 mM) of SR (MedChemExpress, NJ, U.S.A.) were added for 48 h of incubation, and triplicate wells were set in each group. Finally, 20 μl CCK-8 solution was added to each well, and incubation was continued for another 4 h at 37°C, then each well was measured by a multi-plate reader BioTek Synergy 2 (Winooski, VT, U.S.A.). The effect of SR on the cell viability of MC3T3-E1 was evaluated according to OD450 value. The experiment was repeated three times, and the averaged value was calculated for each concentration.
3
Evaluating Dox and SRF Effects on B16F10 and HCC-LM3 Cells
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B16F10‐Luci or HCC‐LM3‐Luci cells (2 × 105 cells/ml) were seeded in 96‐well plates and allowed to attach for 12 h. Then, 0.3 μg/ml Dox, 5 μg/ml Dox, 10 μg/ml RBC‐EVs loaded with Dox, 1.5 μg/ml SRF or 10 μg/ml RBC‐EVs loaded with SRF was added. The viability was assayed at 24 h, 48 h or 72 h by using a Cell Counting Kit‐8 assay (TransGen, Beijing, China).
4
Evaluating Osteosarcoma Cell Proliferation
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The proliferation of OS cells was evaluated by a Cell-Counting Kit-8 (CCK-8, TransGen, Inc., Beijing, China) assay. Osteosarcoma cells (4000/well) were seeded out in 96-well plates for 0 hour, 24 hours, and 48 hours, respectively. Then, 10 µL of CCK-8 solution was added to each well, and incubated for an additional 2 hours at 37°C. The absorbance of each wells was measured at 450 nm with a microplate reader. Three independent experiments were performed over multiple days.
5
Cell Proliferation Assay via CCK-8
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Cell proliferation was detected by Cell Counting Kit-8 (CCK-8; TransGen Biotech Co., Ltd.Beijing, China.) according to the manufacturer's instructions. 1000 cells/well were seeded into 96-well plates and cultured. The 10 uL CCK-8 solution was added every 24 hours. After 2-hour incubation, we detected the absorbency at a test wavelength of 450nm.
6
Evaluating the Cytotoxic Effects of SeO2 and GSK-J4
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The pharmacological effects of SeO2 and GSK-J4 on cell proliferation were assessed using Cell Counting Kit-8 (CCK-8, TransGen, Beijing, China) following the manufacturer's instructions. Briefly, HeLa and SiHa cells were seeded at a density of 3 × 103 cells/well in 96-well culture plates and treated with varying concentrations of SeO2 and GSK-J4. After treatment, 20 μl of CCK-8 solution was added to each well and incubated for an additional 2 h at 37 °C in a 5% CO2 incubator. The optical density (OD) of each well was measured at 450 nm using a microplate reader (BioTek, VT, USA), and the data was graphically displayed. The growth rate (%) was calculated using the formula: (ODExperiment − ODBlank)/(ODControl − ODBlank) × 100%. The IC50 was determined using GraphPad Prism 9 software (GraphPad Software, USA).
7
Light-Induced p53 and Cell Growth
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The effects of light-induced p53 expression on cell growth were determined by Cell Counting Kit-8 assay (Transgene, China). Briefly, 5×103 cells/well were seeded in a 96-well plate for 12h and then transfected as mentioned above (each vector was adjust to 25 ng/well). At 0, 24, 48, 72 hours after blue light illumination, 100 μl fresh medium containing 10% of CCK-8 was replaced to each well and the cells were cultured for 1 hour. The absorbance at 450 nm was determined using an ELISA microplate reader (Bio-Rad, Hercules, CA, USA). Experiments were repeated for three times.
8
Cell Growth Assay with VP and YAP
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The effects of VP and YAP on cell growth were determined by Cell Counting Kit-8 assay (Transgene, China). In brief, 5×103 cells per well were seeded in a 96-well plate for 12h culture and then transfected or treated with corresponding plasmids and/or VP (10 μg/ml) as mentioned previously. After transfection for 48h, 100 μl fresh medium with 10% of CCK-8 was replaced into each well and the cells were cultured for another one hour. The absorbance of 450 nm was detected by using an ELISA microplate reader (Bio-Rad, Hercules, CA, USA). Every experiment was repeated for three times.
9
Cell Viability Assay for Cytotoxic Drugs
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Cytotoxicity
of screened drugs
were evaluated by cell proliferation assay. Briefly, 293T cells were
plated 5000 cells per well in a 96-well plate for 24 h and then treated
with serially diluted drugs for 24 h. Next, the live cell number was
quantified by Cell Counting Kit 8 (CCK8, Transgene). The absorbance
was measured at 450 nm using a microplate spectrophotometer (Multiskan
Go, Thermo Scientific). Experiments were conducted in triplicate,
and cell viability was calculated as the ratio of experiment group
related to the control group.
of screened drugs
were evaluated by cell proliferation assay. Briefly, 293T cells were
plated 5000 cells per well in a 96-well plate for 24 h and then treated
with serially diluted drugs for 24 h. Next, the live cell number was
quantified by Cell Counting Kit 8 (CCK8, Transgene). The absorbance
was measured at 450 nm using a microplate spectrophotometer (Multiskan
Go, Thermo Scientific). Experiments were conducted in triplicate,
and cell viability was calculated as the ratio of experiment group
related to the control group.
10
Assessing Cell Proliferation in ccRCC
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Cell proliferation was determined by an ethynyl-2-deoxyuridine (EdU) incorporation assay using an EdU Apollo DNA in vitro kit (RiboBio, Guangzhou, China) and BeyoClick™ EdU Cell Proliferation Kit with DAB (Beyotime, China) following the manufacturer’s instructions. ccRCC cells were seeded in 96-well plates and cell viability was evaluated with the Cell Counting Kit 8 (TransGen Biotech, Beijing, China). Absorbance was measured (OD value) at a wavelength of 450 nm.
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