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Anti cd43 1b11

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Anti-CD43 (1B11) is a mouse monoclonal antibody that binds to the CD43 antigen. CD43 is a sialoglycoprotein expressed on the surface of most hematopoietic cells, including T cells, B cells, and monocytes. The specific function of this antibody clone is to recognize and bind to the CD43 molecule.

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Lab products found in correlation

Foxp3 fixation and permeabilization kit, by Thermo Fisher Scientific (3 mentions) Anti cd86 gl 1, by BioLegend (3 mentions) Anti cd19 1d3, by BioLegend (2 mentions) Ki67 sola15, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Anti cd138 281.2, by BD (1 mentions) Cd62l mel 14, by BioLegend (1 mentions) Anti cd4 rm4 5, by BioLegend (1 mentions) Anti cd4 rm4 5, by Thermo Fisher Scientific (1 mentions) Anti cd25 pc61, by Thermo Fisher Scientific (1 mentions) Anti nk1.1 pk136, by BioLegend (1 mentions) Anti f4 80 bm8, by BioLegend (1 mentions) Anti ctla 4 uc10 4b9, by BioLegend (1 mentions) Anti cd8a 53 6, by BioLegend (1 mentions) Anti cd28 37, by BioLegend (1 mentions) Anti cd11c hl3, by BD (1 mentions) Anti cd45 30 f11, by BD (1 mentions) Anti cd44 im7, by BD (1 mentions) Anti cd11b m1 70, by BioLegend (1 mentions) Anti mhc class 2 m5 114, by BD (1 mentions)

Close spelling variants of this product

Anti-CD43 (4 citations) CD43 (1B11; (3 citations) Anti-CD43 (Clone 1B11; (2 citations)

4 protocols using anti cd43 1b11

1

Multiparameter Flow Cytometry Analysis

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The following antibodies were used for cell surface staining: anti-CD4 (RM4-5, BioLegend), anti-CD19 (1D3, BioLegend), anti-CD38 (90, eBio), anti-CD138 (281–2, BD), anti-CD86 (Gl1, Biolegend), anti-Ter119 (TER-119, eBio), anti-GL7 (GL-7, eBio), anti-mouse IgD (11–26, eBio) anti-mouse IgM (11/41, eBio), anti-PD1 (J43, eBio), anti-CD43 (1B11, BioLegend), CD62L (MEL-14, BioLegend). Following surface staining, intracellular staining was performed with the FOXP3 permeabilization and fixation kit from eBiosciences according to the manufacturers recommendations using the following antibodies: Ki-67 (SolA15, BD) or FOXP3 (FJK-16s, eBio). FOXP3 expression in cells from FOXP3-DTR mice was also analyzed by GFP expression. Lymphocyte populations were initially gated on the basis of forward scatter versus side scatter. B cells were gated as CD19+CD4- cells within the lymphocyte gate. GC B cells were gated as CD38-GL7+ cells within the B cell gate and plasmablasts were gated as CD38+CD138+ cells within the B cell gate. CD4 T cells were gated as CD4+ cells within the lymphocyte gate. See S1 Fig for gating strategies. Flow cytometic data were collected with an LSRII (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Moore T.C., Messer R.J, & Hasenkrug K.J. (2018). Regulatory T cells suppress virus-specific antibody responses to Friend retrovirus infection. PLoS ONE, 13(4), e0195402.
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2

Multiparametric Flow Cytometry Analysis

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The harvested spleens were mashed and single-cell suspensions of the spleens were stained with the following surface antibodies: anti-CD45 (30-F11, BD Biosciences), LIVE/DEAD Fixable Blue cell stain (Invitrogen), anti-CD19 (ID3, BD Biosciences), anti-CD8a (53-6.7, BioLegend), anti-CD27 (LG7F9, eBioscience), anti-CD11b (M1/70, BioLegend), anti-CD11c (HL3, BD Biosciences), anti-CD86 (GL1, BioLegend), anti-NK1.1 (PK136, BioLegend), anti-NKp46 (29A1.4, BioLegend), anti-MHC Class II (M5/114.15.2, BD Biosciences), anti-F4/80 (BM8, BioLegend), anti-CD80 (16-10A1), anti-CD3 (17A2, BioLegend), anti-CD4 (RM4-5, Invitrogen), anti-CTLA-4 (UC10-4B9, BioLegend), anti-PD-1 (29F.1A12, eBioscience), anti-CD28 (37.51, BioLegend), anti-CD44 (IM7, BD Biosciences), anti-CD43 (1B11, BioLegend), anti-CD47 (miap301, BioLegend), anti-CD62L (MEL-14, BioLegend), anti-CD25 (PC61.5, eBioscience), and anti-CD107a (1D4B, BioLegend). Intracellular staining was then performed with a FOXP3 permeabilization and fixation kit (eBioscience) according to manufacturer’s recommendations using the following antibodies: Ki-67 (B56; BD Biosciences) and GrB (QA16A02, BioLegend). Flow cytometric data were collected with a Beckman Coulter Cytoflex LX (6-L NUV) flow cytometer and analyzed using FlowJo software (Tree Star).
Rao D., O’Donnell K.L., Carmody A., Weissman I.L., Hasenkrug K.J, & Marzi A. (2021). CD47 expression attenuates Ebola virus-induced immunopathology in mice. Antiviral research, 197, 105226.
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3

Multiparameter Flow Cytometry Analysis

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The following antibodies were used for cell surface staining: monoclonal antibody 34 (mAb34) against FV glycogag, anti-CD19 (1D3; BioLegend), anti-CD8 (53-6.7; BD), anti-CD43 (1B11; BioLegend), anti-CD11a (LFA1; BioLegend), anti-CD44 (1M7; BioLegend), anti-Ter119 (TER-119; eBio), anti-CD11b (M1/70; eBio), and Db GagL dextramer (Immudex). Following surface staining, intracellular staining was performed with a FOXP3 permeabilization and fixation kit from eBiosciences according to the recommendations of the manufacturer using the following antibodies: Ki-67 (SolA15; BD) and GrB (GB11; Invitrogen). Lymphocyte populations were initially gated on the basis of forward scatter versus side scatter. Flow cytometric data were collected with an LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star). FACS analysis was performed on a FACSAria instrument (BD Biosciences).
Moore T.C., Gonzaga L.M., Mather J.M., Messer R.J, & Hasenkrug K.J. (2017). B Cell Requirement for Robust Regulatory T Cell Responses to Friend Retrovirus Infection. mBio, 8(4), e01122-17.
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4

Flow Cytometric Analysis of Activated Immune Cells

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Cell surface staining was performed using the following antibodies: anti-CD11b (M1/70, Miltenyi Biotec), anti-CD11c (N418, BioLegend) anti-CD3 (17A2, eBioscience), anti-CD49b (DX5, eBioscience), anti-CD69 (H1.2 F3, eBioscience), anti-CD86 (GL-1, BioLegend), anti-MHC-II (M5/114.15.2, Miltenyi Biotec), anti-NK1.1 (PK136, BD Bioscience). Dead cells were excluded from analysis (positive for fixable viability dye, eBioscience). For detection of activated T cells splenocytes were stained with anti-CD4 (GK1.5, eBioscience), anti-CD8 (53–6.7, eBioscience), anti-CD43 (1B11, BioLegend) and anti-CD62L (MEL-14, eBioscience). Intracellular IFN-γ (XMG1.2, Miltenyi Biotec), IL-2 (JES6-5H4, Miltenyi Biotec) and TNF-α (MP6-XT22, BioLegend) staining was performed as described [28 (link),29 (link)]. Data were acquired on LSR II flow cytometer (BD Bioscience) and analyses were performed using FACSDiva (BD Bioscience) and Flow Jo (Tree Star, USA) software.
Gibbert K., Francois S., Sigmund A.M., Harper M.S., Barrett B.S., Kirchning C.J., Lu M., Santiago M.L, & Dittmer U. (2014). Friend retrovirus drives cytotoxic effectors through Toll-like receptor 3. Retrovirology, 11, 126.
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