Alamethicin and uridine 5′-diphosphoglucuronic acid trisodium salt (UDPGA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Deuterated DMSO and methanol were purchased from Cambridge Isotope Laboratories (Tewksbury, MA, USA). UGT Reaction Mix - Solution B, pooled human liver microsomes and recombinant human UGTs 1A1, 1A3, 1A4, 1A6, 1A8–10, 2B4, 2B7, 2B15 and 2B17 were purchased from BD Gentest (Woburn, MA, USA). High-performance liquid chromatography-grade solvents and analytical-grade reagents were used in all experiments.
Methanol
Manufactured by Cambridge Isotopes
Sourced in United States
Methanol is a clear, colorless liquid chemical compound. It is the simplest alcohol, with the chemical formula CH3OH. Methanol is commonly used as a solvent, fuel, and feedstock for the production of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Inosine 15n4, by Cambridge Isotopes (2 mentions)
Thymine d4, by Cambridge Isotopes (2 mentions)
Glycocholate d4, by Cambridge Isotopes (2 mentions)
Deuterated dmso, by Cambridge Isotopes (1 mentions)
Uridine 5 diphosphoglucuronic acid trisodium salt udpga, by Merck Group (1 mentions)
Alamethicin, by Merck Group (1 mentions)
Pooled human liver microsomes, by BD (1 mentions)
13c methanol, by Cambridge Isotopes (1 mentions)
Luna nh2 column, by Phenomenex (1 mentions)
Lithium heparin, by Thermo Fisher Scientific (1 mentions)
Methanol, by Restek (1 mentions)
Purelab options7, by Elga LabWater (1 mentions)
Benzalkonium chloride, by Thermo Fisher Scientific (1 mentions)
Butylated hydroxyanisole, by Merck Group (1 mentions)
Cetrimide, by Merck Group (1 mentions)
Deuterated chloroform, by Cambridge Isotopes (1 mentions)
Carbopol 940, by Thermo Fisher Scientific (1 mentions)
6 protocols using methanol
1
Characterization of UGT Isoenzyme Activity
2
EPR Characterization of Mn(III,IV)salpn-Methanol Complexes
3
Negative Ion Mode MS of Plasma Metabolites
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Negative ion mode MS analysis of polar metabolites. LC–MS samples were prepared from plasma (30 μl) via protein precipitation with the addition of four volumes (120 μl) of 80% methanol containing inosine‐15N4, thymine‐d4, and glycocholate‐d4 internal standards (Cambridge Isotope Laboratories). The samples were centrifuged (10 min, 9000 g, 4°C), and the supernatants were injected directly onto a 150 × 2.0 mm Luna NH2 column (Phenomenex). The column was eluted at a flow rate of 400 μl/min with initial conditions of 10% mobile phase A (20 mM ammonium acetate and 20 mM ammonium hydroxide in water) and 90% mobile phase B (10 mM ammonium hydroxide in 75:25 v/v acetonitrile/methanol) followed by a 10 min linear gradient to 100% mobile phase A. The column temperature was kept at 40°C. MS analyses were carried out using electrospray ionization in the negative ion mode using full scan analysis over m/z 70–750 at 70,000 resolution and 3 Hz data acquisition rate. Additional MS settings are as follows: ion spray voltage, −3.0 kV; capillary temperature, 350°C; probe heater temperature, 325°C; sheath gas, 55; auxiliary gas, 10; and S‐lens RF level 50.
4
Plasma Metabolite Extraction Protocol
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Mice were culled immediately by cervical dislocation. Blood was taken via cardiac puncture, collected into a microtainer tube lined with lithium heparin (Fisher Scientific), and centrifuged (10 min, maximum speed, 4°C). Plasma was subsequently snap frozen. Metabolites were extracted from plasma by adding extraction buffer, consisting of 80% methanol containing inosine-15N4, thymine-d4, and glycocholate-d4 internal standards (Cambridge Isotope Laboratories) in a 1:4 ratio of plasma to extraction buffer. Samples were then centrifuged twice (5 min, maximum speed, 4°C), and supernatants were collected.
5
Quantification of Acrylamide using Stable Isotope
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Native AA of concentration 1004 µg/mL in methanol (99% purity) was purchased from Restek (Benner Circle, Bellefonte, U.S.) and labelled acrylamide (1,2,3-13 C labelled AA) (99% purity) of concentration 1000 µg/mL in methanol (+100 ppm hydroquinone) used as internal standard (IS) was acquired from Cambridge Isotope Laboratories (Andover, MA, USA). All solvents and reagents were of analytical grade, special for chromatography and were purchased from Merck (Darmstadt, Germany), LGC Promochem GmbH (Wesel, Germany), Alfa Aesar (Thermo Fisher Scientific, USA). Ultrapure water was obtained through a PURELAB Option-S7 and PURELAB Ultra Ionic system (Elga Labwater, High Wycombe, UK).
To assess the quality control, a certified reference material "acrylamide in rusk" (ERM ® -BD274, BAM, Berlin, Germany) with the certified value of 74 μg/kg was used.
To assess the quality control, a certified reference material "acrylamide in rusk" (ERM ® -BD274, BAM, Berlin, Germany) with the certified value of 74 μg/kg was used.
6
Formulation and Characterization of Omega-3 Enriched Topical Gel
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Fish oil from menhaden (crude source of omega-3 fatty acids), cis-eicosapentaenoic acid analytical standard, cis-docosahexaenoic acid analytical standard, cetrimide, and butylated hydroxyanisole (BHA) were purchased from Sigma-Aldrich (USA). CoQ10 powder was purchased from Beijing Wisapple Biotech Co., Ltd (China). Methanol and deuterated chloroform were purchased from Cambridge Isotope Laboratories (USA), and beeswax was obtained from R&M Chemicals (Selangor, Malaysia). Benzalkonium chloride and carbopol 940 were purchased from ACROS (New Jersey, USA), triethanolamine was obtained from Friendemann Schmidt (Parkwood, WA, Australia), and porcine ears were obtained from an abattoir in Ipoh, Malaysia.
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