Venous blood sample were drawn from subjects after they had fasted overnight. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, glucose, high-sensitivity C-reactive protein and lipoprotein (a) levels were measured using an automatic biochemistry analyzer, ARCHITECT c8000 (Abbott Laboratories, Abbott Park, IL, USA). Low-density lipoprotein cholesterol was calculated according to the Friedewald formula [21 (link)]. Homocysteine levels were determined using an AxSYM immunoassay analyzer (Abbott Laboratories, Abbott Park, IL, USA). Fibrinogen levels were measured using an ACL ELife Pro coagulation analyzer (IL, Milan, Italy).
Axsym immunoassay analyzer
Manufactured by Abbott
Sourced in United States
The AxSYM immunoassay analyzer is a laboratory equipment used for automated processing and analysis of immunoassay tests. It performs various immunoassay procedures, such as enzyme immunoassay (EIA) and fluorescence polarization immunoassay (FPIA), to detect and measure specific analytes in biological samples.
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Lab products found in correlation
3 protocols using axsym immunoassay analyzer
1
Lipid and Inflammatory Biomarker Analysis
2
Urinary Biomarkers in Anesthesia
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Briefly, 24-hour urine was collected before anesthesia. The level of microalbumin (MAlb) was detected by immune transmission (Hitachi7600, Hitachi, Tokyo, Japan). The level of alpha-1 microglobulin (α1-MG) was detected by immune rate nephelometry (protein chemistry analyzer IMMAGE800, Backman Coulter). The level of beta-2 microglobulin (β2-MG) was detected by microparticle immunoluminescence (AXSYM immunoassay analyzer, Abbott, Abbott Park, IL). The level of N-acetyl-beta-D-glucosidase (NAG) was detected by immunoturbidimetry (Synergy H4, BioTek, Winooski, VT).
3
Comprehensive Blood Biomarker Analysis
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A volume of 10 ml of fasting blood sample was collected in EDTA vacutainers. Haematological parameters were measured on a five-part differential cell counter (Sysmex) and the remaining blood was centrifuged at 1500 g for 20 min. Separated plasma was stored for biochemical investigations at -20°C. Plasma holo-TC was measured using microparticle enzyme immunoassay. Microparticle enzyme intrinsic factor assay was used for the quantitative determination of plasma vitamin B 12 . Plasma folate and tHcy were measured by the fluorescence polarisation immunoassay technique. All four biomarkers were analysed on an AxSYM immunoassay analyzer (Abbott Laboratories) (17) . Plasma creatinine was measured using the alkaline picrate method with a Daytona analyser (Randox) (14) .
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