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Metamorph premier

Manufactured by Molecular Devices
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Sourced in Japan, United States

MetaMorph Premier is a comprehensive software package designed for advanced imaging and analysis. It provides a powerful platform for controlling automated microscopes and capturing high-quality images. MetaMorph Premier offers a range of tools for tasks such as image acquisition, processing, and analysis.

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Lab products found in correlation

5 cy3 oo, by Panagene (2 mentions) 4 3 probe, by Panagene (2 mentions) 35 mm glass bottomed microwell dish, by MatTek (1 mentions) Orca 2 ccd camera, by Hamamatsu Photonics (1 mentions) 100 w highpressure mercury burner, by Olympus (1 mentions) Metamorph premier software, by Molecular Devices (1 mentions) Disc spinning unit, by Olympus (1 mentions)

Close spelling variants of this product

MetaMorph (766 citations) MetaMorph Premier software (6 citations) MetaMorph Premier Image Analysis Software (2 citations) MetaMorph Premier version 7.5.2 (2 citations)

11 protocols using metamorph premier

1

Telomere Length Analysis by PNA-FISH

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Cells were treated with 0.5 μg/ml of Colcemid for 4–7 h before harvest. Chromosomes were fixed and telomere PNA-FISH performed with a 5′-Cy3-OO-(CCCTAA)4-3′ probe (PANAgene) as described 14 (link), 63 (link). For CO-FISH, metaphase spreads were incubated sequentially with 5′-Cy3-OO-(CCCTAA)4-3′ and 5′-FAM-CO-(TTAGGG)4-3′ probes. Images were captured on a Nikon Eclipse 800 microscope and processed with MetaMorph Premier (Molecular Devices).
Gu P., Wang Y., Bisht K.K., Wu L., Kukova L., Smith E.M., Xiao Y., Bailey S., Lei M., Nandakumar J, & Chang S. (2016). Human Pot1 OB-fold mutations unleash rampant telomere instability to initiate tumorigenesis. Oncogene, 36(14), 1939-1951.
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2

Chromosome Fusion Analysis by PNA-FISH

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Cells were treated with 0.5 μg ml−1 of Colcemid for 4–7 h before chromosome harvest. Metaphase spreads were fixed in 4% formaldehyde in PBS for 10 min and then dehydrated with sequential rinses in 70, 85 and 100% ethanol for 2 min each and air dried. Telomere PNA-FISH was performed with 10 ng μl−1 5′-Tam-OO-(CCCTAA)4-3′ probe in PNA-FISH hybridization mix (2% BSA, 100 μg ml−1 tRNA, 0.6 × SSC, 100% formamide)66 (link)67 (link). For CO-FISH, fixed metaphase spreads were incubated sequentially with 10 ng μl−1 5′-Tam-OO-(CCCTAA)4-3′ and 10 ng μl−1 5′-FITC-CO-(TTAGGG)4-3′ probes in PNA-FISH hybridization mix24 (link). All images were captured on a Nikon Eclipse 800 microscope and processed with MetaMorph Premier (Molecular Devices). The percent of chromosome fusions observed is defined as: total number of chromosome fusions in 30–50 metaphase spreads analysed divided by the total number of chromosomes examined × 100%. Telomere-free fusions are defined as end-to-end chromosome fusions without any telomeric signals at sites of fusion.
Rai R., Chen Y., Lei M, & Chang S. (2016). TRF2-RAP1 is required to protect telomeres from engaging in homologous recombination-mediated deletions and fusions. Nature Communications, 7, 10881.
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3

Quantifying Leukocyte-Endothelial Interactions in IVC

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Leukocyte-endothelial interactions were measured similar to our previously described experiments involving cremasteric venules40 (link). For IVC analysis, 200 µl rhodamine-6G (0.067 mg/ml) was injected intravenously via the tail vein 2 hours following partial IVC ligation. The abdomen was then reopened and labeled leukocytes were visualized with a Nikon FN1 fixed-stage microscopy system with X-cite for epi-fluorescence. The area of interest consisted of a 2-3 mm segment of IVC distal to the IVC ligation. Videos were recorded with a Photometrics Coolsnap Cascade 512B color digital camera system for 2 minutes and analyzed with the MetaMorph Premier software package (Molecular Devices). After intravital microscopy, mice were sacrificed with intraperitoneal (i.p.) injection of sodium pentobarbital (100 mg/kg). Adherent cells were defined by lack of movement for at least 2 minutes. The number of adherent cells was manually counted as overlaid cells (yellow) in pictures taken at 0 min (assigned green) and 2 min (assigned red) of recording.
Wang H., Wang Q., Wang J., Guo C., Kleiman K., Meng H., Knight J.S, & Eitzman D.T. (2017). Proprotein convertase subtilisin/kexin type 9 (PCSK9) Deficiency is Protective Against Venous Thrombosis in Mice. Scientific Reports, 7, 14360.
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4

Melanin Quantification in Keratinocytes

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Images were obtained by automated microscopy (Olympus, automated stage, ZDC autofocus, Tokyo, Japan) and were analyzed by Metamorph Premier software (Molecular Device LLC, Sunnyvale, CA). First, the backgrounds (keratinocytes-only) were subtracted from each image and thresholds were determined manually, to detect pigmented areas using forskolin (FSK) (pro-pigmentation) and phenylthionuria (PTU) (anti-pigmentation) controls.
Lee J.H., Chen H., Kolev V., Aull K.H., Jung I., Wang J., Miyamoto S., Hosoi J., Mandinova A, & Fisher D.E. (2014). High Throughput, High Content Screening for Novel Pigmentation Regulators Using a Keratinocyte/Melanocyte Co-culture System. Experimental dermatology, 23(2), 125-129.
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5

Angiotensin II-Induced Wound Healing

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PASMCs were seeded on 24-well plates. At 80% to 90% confluency, cells were serum starved for 24 hours and scratches were made using a 20-μL pipette tip. Cells were washed with Dulbecco’s PBS (without calcium and magnesium) and stimulated with basal smooth muscle cell medium containing 1 μM AngII, 10 μM TRV023 with or without 10 μM losartan. The wound closure was monitored using a live-cell station Zeiss Axio Observer microscope (Duke Light Microscopy Core Facility). The images were captured hourly in real time from 0 to 12 hours. The initial edges of the scratch at 0 hour were marked and migrated distance at 12 hours was measured using MetaMorph Premier (Molecular Devices).
Ma Z., Viswanathan G., Sellig M., Jassal C., Choi I., Garikipati A., Xiong X., Nazo N, & Rajagopal S. (2021). β-Arrestin–Mediated Angiotensin II Type 1 Receptor Activation Promotes Pulmonary Vascular Remodeling in Pulmonary Hypertension. JACC: Basic to Translational Science, 6(11), 854-869.
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6

Enhancing Confocal Microscopy Imaging

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Sections affixed to slides were viewed on an Olympus DSU (disc scanning unit) confocal microscope equipped with a Q-Fire digital camera, as well as DAPI and mCherry filters and a Photometrix QuantEm 512 SC camera for fluorescent microscopy image capture. MetaMorph Premier (Molecular Devices, Sunnyvale, CA, USA) imaging software in conjunction with ImageJ (U.S. NIH, Bethesda, MD, USA) and Adobe Photoshop software were used to acquire and process images. The Smart Sharpen filter and Brighten/Contrast tools in Photoshop CS4 were used to enhance contrast in TEM images and to correct brightness levels in brightfield images.
Lynn G.E., Oliver J.D., Nelson C.M., Felsheim R.F., Kurtti T.J, & Munderloh U.G. (2015). Tissue Distribution of the Ehrlichia muris-Like Agent in a Tick Vector. PLoS ONE, 10(3), e0122007.
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7

Telomere Length Analysis by PNA-FISH

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Cells were treated with 0.5 μg/ml of Colcemid for 4–7 h before harvest. Chromosomes were fixed and telomere PNA-FISH performed with a 5′-Cy3-OO-(CCCTAA)4-3′ probe (PANAgene) as described 14 (link), 63 (link). For CO-FISH, metaphase spreads were incubated sequentially with 5′-Cy3-OO-(CCCTAA)4-3′ and 5′-FAM-CO-(TTAGGG)4-3′ probes. Images were captured on a Nikon Eclipse 800 microscope and processed with MetaMorph Premier (Molecular Devices).
Gu P., Wang Y., Bisht K.K., Wu L., Kukova L., Smith E.M., Xiao Y., Bailey S., Lei M., Nandakumar J, & Chang S. (2016). Human Pot1 OB-fold mutations unleash rampant telomere instability to initiate tumorigenesis. Oncogene, 36(14), 1939-1951.
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8

Quantifying Focal Adhesion Size

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Images were taken on the Olympus IX81 as described above, converted to grey scale and processed with a high-band pass filter using Metamorph Premier (v 7.7.2.0, Molecular Devices, Sunnyvale, CA) to sharpen and enhance the focal adhesions. Focal adhesion size was determined by tracing a line along the length of the adhesion of interest and measured using the ‘Measure’ feature in Image J similar to the procedure described previously [29] (link). A minimum of 90 adhesions were analyzed per condition.
Janson I.A., Kong Y.P, & Putnam A.J. (2014). Nanotopographic Substrates of Poly (Methyl Methacrylate) Do Not Strongly Influence the Osteogenic Phenotype of Mesenchymal Stem Cells In Vitro. PLoS ONE, 9(3), e90719.
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9

Peptide Internalization in H1299 Cells

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A total of 1
mL of H1299 cell suspension (∼1.5 × 104 cells)
was seeded in a 35 mm glass-bottomed microwell dish (MatTek) and cultured
overnight. Cells were gently washed with PBS twice and treated with
FITC-labeled peptide (5 μM) in phenol-red free and HEPES supplemented
RPMI containing 10% FBS at 37 °C for 2 h in the presence of 5%
CO2. After removal of the medium, the cells were gently
washed with DPBS twice and imaged on a Visitech Infinity 3 Hawk 2D-array
live cell imaging confocal microscope equipped with 60× oil objective.
Data were analyzed using MetaMorph Premier (Molecular Devices).
Trinh T.B., Upadhyaya P., Qian Z, & Pei D. (2015). Discovery of a Direct Ras Inhibitor by Screening a Combinatorial Library of Cell-Permeable Bicyclic Peptides. ACS Combinatorial Science, 18(1), 75-85.
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10

Quantifying Cell Population Responses to Clodronate

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SCs (10,000 cells/cm2), BMDMs (10,000 cells/ cm2), HUVEC (10,000 cells/ cm2), or DRGs (500 neurons/ cm2) were seeded onto 48-well plates in their respective culture medium. Two days later, clodronate liposome of various concentration were added to respective culture media and incubated for 48 hrs at 37°C. Cells were then fixed in 4% PFA and stained with primary antibodies to S100 (SCs), Iba1 (BMDMs), vWF (HUVECs), or βIII-tubulin (DRG), followed by respective secondary antibody with Alexa-555 conjugation. Stained cells were imaged using an Olympus IX81 microscope (Olympus) using a cooled, charged-coupled device (CCD) camera with MetaMorph Premier version 7.6.5.0 (Molecular Devices). Four random fields were sampled at 20x magnification for each well (replicate. Images were analyzed on ImageJ (NIH), and area of stain were quantified by first converting the images to binary before performing the “particle analysis” function to determine the percentage of stained area (primary antibody positive; DAPI was not included) for the entire imaged area. An average from the four images was obtained to represent a replicate), where 4 replicates were performed per condition.
Pan D., Sayanagi J., Acevedo-Cintron J.A., Schellhardt L., Snyder-Warwick A.K., Mackinnon S.E, & Wood M.D. (2020). Liposomes embedded within fibrin gels facilitate localized macrophage manipulations within nerve. Journal of neuroscience methods, 348, 108981.
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