Uv 4075 uv vis detector

Manufactured by Jasco

Sourced in Japan

The UV-4075 UV/VIS detectors are a series of spectrophotometric instruments designed for the measurement of absorbance, transmittance, and concentration of samples in the ultraviolet and visible light spectrum. These detectors utilize a deuterium and tungsten-halogen light source to cover the wavelength range from 190 to 1100 nanometers. The UV-4075 models offer optical resolution and wavelength accuracy suitable for a variety of analytical applications.

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Lab products found in correlation

Pu 4180 pump, by Jasco (4 mentions) Pu 4080i infusion pump, by Jasco (3 mentions) Chromnav software, by Jasco (2 mentions) Superose 6 increase 10 300 gl column, by GE Healthcare (2 mentions) Pu 4180 rhplc pump, by Jasco (2 mentions) Cobas 6000, by Roche (1 mentions) Precimat glycerol, by Roche (1 mentions) Superose 6 increase 10 300 gl, by GE Healthcare (1 mentions) Cobas 6000 analyzer, by Roche (1 mentions) Enzymatic kit, by DiaSys (1 mentions) Jms t100 gcv, by JEOL (1 mentions) Multiskan microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions) Ascend 400 mhz nmr spectrometer, by Bruker (1 mentions) X ray tube, by Bruker (1 mentions) U shape microplates, by Greiner (1 mentions) Ltq orbitrap xl mass spectrometer, by Thermo Fisher Scientific (1 mentions) Lc net 2 adc controller, by Jasco (1 mentions) Inca x sight, by Oxford Instruments (1 mentions) 2100f microscope, by JEOL (1 mentions) Optima 2100 dv instrument, by PerkinElmer (1 mentions)

Spelling variants of this product

UV-4075 (14 citations) UV detector (7 citations) UV-vis detector (6 citations) UV-4075 detector (3 citations) UV-4075 UV/Vis (2 citations)

7 protocols using uv 4075 uv vis detector

Comprehensive Plasma Lipid and Lipoprotein Analysis

Cited 1 time

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Plasma triglycerides (Roche Diagnostics), total cholesterol, free cholesterol, nonesterified FAs (all DiaSys Diagnostic Systems) were quantified using commercially available reagents.
Plasma lipoproteins were separated by fast protein liquid chromatography using a system containing a PU-4180 pump with a linear degasser and UV-4075 UV/VIS detectors (Jasco, Tokyo, Japan), as described (12 (link)). Plasma plant sterols were determined by GC as described (26 (link)). Circulating levels of aspartate aminotransferase, alanine aminotransferase, and albumin were determined using a routine clinical chemistry analyzer (Cobas 6000; Roche Diagnostics) with standard reagents (Roche Diagnostics).

Li R., Palmiotti A., de Vries H.D., Hovingh M.V., Koehorst M., Mulder N.L., Zhang Y., Kats K., Bloks V.W., Fu J., Verkade H.J., de Boer J.F, & Kuipers F. (2021). Low production of 12α-hydroxylated bile acids prevents hepatic steatosis in Cyp2c70−/− mice by reducing fat absorption. Journal of Lipid Research, 62, 100134.

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Plasma Lipid Profiling by FPLC

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Blood samples were collected. Plasma was separated by centrifugation and plasma triglyceride (TG) and free fatty acid (FFA) levels were measured using enzymatic kits (157.109.910.917 and 157.819.910.935, respectively; Diasys Diagnostic Systems, Holzheim, Germany) with Precimat Glycerol or FFA standard FS (10,166,588; Roche, Mannheim, Germany and 157.809.910.065; Diasys Diagnostic Systems, respectively) for the calibration curve. Lipoprotein TG distribution was measured by fast performance liquid chromatography (FPLC) using a system containing a PU-4180 pump with a linear degasser and UV-4075 UV/VIS detectors (Jasco, Tokyo, Japan). Pooled plasma samples (n = 8–12) were injected onto a Superose 6 Increase 10/300 GL column (GE Healthcare, Hoevelaken, The Netherlands) and eluted at a constant flow rate of 0.31 ml/min in PBS (pH 7.4). Triglycerides were measured in line by the addition of TG reagent (157.109.910.917; Diasys, Holzheim, Germany) at a constant flow rate of 0.1 ml/min using an additional PU-4080i infusion pump (Jasco, Tokyo, Japan). Data acquisition and analysis were performed using ChromNav software (version 1.0; Jasco, Tokyo, Japan).

La Rose A.M., Bazioti V., Hoogerland J.A., Svendsen A.F., Groenen A.G., van Faassen M., Rutten M.G., Kloosterhuis N.J., Dethmers-Ausema B., Nijland J.H., Mithieux G., Rajas F., Kuipers F., Lukens M.V., Soehnlein O., Oosterveer M.H, & Westerterp M. (2021). Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia. Molecular Metabolism, 53, 101265.

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Plasma Lipid Profile Quantification

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Plasma triglycerides, free fatty acids, total cholesterol, and free cholesterol were measured using commercially available kits (DiaSys Diagnostic Systems, Holzheim, Germany; Roche Diagnostics, Basel, Switzerland). Plasma lipoproteins were separated by fast protein liquid chromatography using a system containing a PU-4180 pump with a linear degasser and UV-4075 UV/VIS detectors (Jasco, Tokyo, Japan), as described.³⁸ (link) Plasma (25 μL) was diluted with phosphate-buffered saline (PBS) (pH 7.4) in a 1:1 ratio before being loaded onto the column (Superose 6 Increase 10/300 GL; GE Healthcare, Hoevelaken, the Netherlands). Lipoproteins were then separated using PBS (pH 7.4, flow rate of 0.31 mL/min) as eluent. Total cholesterol concentrations were quantified using a colorimetric reagent (11489232; Roche Diagnostics) that was added in line at a rate of 0.1 mL/min using an additional PU-4080i infusion pump (Jasco). Data acquisition and analysis were performed using ChromNav software (version 1.0; Jasco).
Plasma transaminases were analyzed using a Cobas 6000 analyzer with standard reagents (Roche Diagnostics). Endotoxin was measured using Endosafe limulus amebocyte lysate cartridges for the Nexgen-PTS (Charles River, Leiden, the Netherlands) after a dilution of 30× in limulus amebocyte lysate water.

de Boer J.F., de Vries H.D., Palmiotti A., Li R., Doestzada M., Hoogerland J.A., Fu J., La Rose A.M., Westerterp M., Mulder N.L., Hovingh M.V., Koehorst M., Kloosterhuis N.J., Wolters J.C., Bloks V.W., Haas J.T., Dombrowicz D., Staels B., van de Sluis B, & Kuipers F. (2020). Cholangiopathy and Biliary Fibrosis in Cyp2c70-Deficient Mice Are Fully Reversed by Ursodeoxycholic Acid. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1045-1069.

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Plasma Cholesterol Profiling in Mice

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Blood samples were collected from mice. Plasma was separated by centrifugation, and cholesterol levels were measured using an enzymatic kit (catalog no.: 113009910026; Diasys Diagnostic Systems, Holzheim, Germany) with Cholesterol FS standard (catalog no.: 113009910030; Diasys Diagnostic Systems) for the calibration curve. Lipoprotein cholesterol distribution was measured by fast performance liquid chromatography using a system containing a PU-4180 pump with a linear degasser and UV-4075 UV/VIS detectors (Jasco, Tokyo, Japan). Pooled plasma samples (n = 15–16 mice per pool) were injected onto a Superose 6 Increase 10/300 GL column (GE Healthcare, Hoevelaken, The Netherlands) and eluted at a constant flow rate of 0.31 ml/min in PBS (pH 7.4). Cholesterol was measured in line by addition of cholesterol reagent at a constant flow rate of 0.1 ml/min using an additional PU-4080i infusion pump (Jasco, Tokyo, Japan). Data acquisition and analysis were performed using ChromNav software (version 1.0; Jasco, Tokyo, Japan).

Groenen A.G., La Rose A.M., Li M., Bazioti V., Svendsen A.F., Kloosterhuis N.J., Ausema A., Pranger A., Heiner-Fokkema M.R., Niezen-Koning K.E., Houben T., Shiri-Sverdlov R, & Westerterp M. (2022). Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease. Journal of Lipid Research, 63(2), 100167.

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Multimodal Characterization of Bioactive Compounds

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The X-ray intensity data were measured on a Bruker D8 goniometer system equipped with a Bruker Turbo X-ray Source rotating-anode X-ray tube (MoKa, λ = 0.71073 Å) and a multilayered confocal mirror monochromator. ¹H- and ¹³C-NMR spectra were acquired on a Bruker Ascend 400 MHz NMR spectrometer (BRUKER BioSpin, Faellanden, Switzerland) at 400 and 125 MHz, respectively. LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a source of electrospray ionization (ESI) was employed to get ESI-MS spectra of pure compounds. A Jasco HPLC system consisting of PU-4180 RHPLC pump, LC-Net II/ADC controller, and UV-4075 UV/Vis detector (Jasco, Tokyo, Japan) and the GC–MS system (JMS-T100 GCV, JEOL Ltd., Tokyo, Japan) were used to identify bioactive constituents. Biological activities were in vitro assayed by using a Multiskan^TM microplate spectrophotometer (Thermo Fisher Scientific, Osaka, Japan) and U-shape microplates (Greiner Bio-one, Monroe, NC, USA). Reagents, solvents, and chemicals at high grades were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan), Junsei Chemical Co., Ltd. (Tokyo, Japan), Fisher Scientific company (Hampton, NH, USA) and Sigma-Aldrich (St. Louis, MO, USA).

Quan N.V., Xuan T.D., Anh L.H, & Tran H.D. (2019). Bio-Guided Isolation of Prospective Bioactive Constituents from Roots of Clausena indica (Dalzell) Oliv. Molecules, 24(24), 4442.

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Plasma Lipoprotein Analysis by FPLC

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Total cholesterol and triglyceride content of the major lipoprotein classes (VLDL, LDL and HDL) were measured using FPLC analysis. In short, the system consisted of a PU-4180 RHPLC pump and a UV-4075 UV-Vis detector (Jasco). Plasma samples of each experimental group of mice were pooled, diluted in PBS and loaded onto a Superose® Increase 10/300 GL column (GE Healthcare) for separation of lipoproteins at a flow rate of 0.31 mL/min. A second flow was used to add the cholesterol (Roche, #1489232) enzymatic reagent at a flow rate of 0.10 mL/min.

Verzijl C.R., Oldoni F., Loaiza N., Wolters J.C., Rimbert A., Tian E., Yang W., Struik D., Smit M., Kloosterhuis N.J., Fernandez A.J., Samara N.L., Ten Hagen K.G., Dalal K., Chernish A., McCluggage P., Tabak L.A., Jonker J.W, & Kuivenhoven J.A. (2022). A novel role for GalNAc-T2 dependent glycosylation in energy homeostasis. Molecular Metabolism, 60, 101472.

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Nanohybrid Characterization Protocol

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X-ray diffraction (XRD) patterns were obtained using a Texture
Analysis D8 ADVANCE Diffractometer (Bruker, Billerica, MA, USA) with
Cu Kα radiation. Scanning electron microscopy (SEM) imaging
was performed on a TM-1000 microscope (Hitachi, Tokyo, Japan). Transmission
electron microscopy (TEM) and high-resolution TEM microscopy (HR-TEM)
images were obtained on a 2100F microscope (JEOL, Tokyo, Japan) equipped
with an EDX detector INCA x-sight (Oxford Instruments, Abingdon, UK).
Interplanar spacing in the nanostructures was calculated by using
the inversed Fourier transform (FT) with the GATAN digital micrograph
program (Corporate Headquarters, Pleasanton, CA, USA). Spectrophotometric
analyses were run on a V-730 spectrophotometer (JASCO, Tokyo, Japan).
Inductively coupled plasma–optical emission spectrometry (ICP–OES)
was performed on an OPTIMA 2100 DV instrument (PerkinElmer, Waltham,
MA, USA). To recover the bionanohybrids, a Biocen 22 R (Orto-Alresa,
Ajalvir, Spain) refrigerated centrifuge was used. Chromatographic
analyses were run at 25 °C using a high-performance liquid chromatography
(HPLC) pump PU-4180 (JASCO, Tokyo, Japan) and a UV-4075 UV–vis
detector (JASCO, Tokyo, Japan).

Ortega-Nieto C., Losada-Garcia N., Pessela B.C., Domingo-Calap P, & Palomo J.M. (2023). Design and Synthesis of Copper Nanobiomaterials with Antimicrobial Properties. ACS Bio & Med Chem Au, 3(4), 349-358.

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