Rat anti mouse cd 11b antibody

LPS (Escherichia coil O111:B4) used for cell culture studies was purchased from Calbiochem (San Diego, CA; cat# 437627) and for animal studies was purchased from Sigma-Aldrich (St. Louis, Mo; cat# L3012). Anti-tyrosine hydroxylase (TH) and anti-Iba-1 were purchased form EMD Millipore (Burlington, MA) and Wako (Richmond, VA), respectively. The secondary antibodies were purchased from Vector Laboratories (Burlingame, CA). The rat anti-mouse CD-11b antibody was purchased from abD Serotec (Raleigh, NC, cat# MCA711G). Anti-pro-IL-1β, anti-alpha-synuclein (α-synuclein), and anti-3-Nitrotyrosine (3-NT) antibodies were purchased from Abcam (Cambridge, MA). Mouse interleukin-1 receptor antagonist (IL-1Ra), NLRP3 inhibitor MCC950, caspase-1 inhibitor Z-YVAD, IRE1α (inositol-requiring enzyme 1α) inhibitor 4μ8C, TNF-α, and IL-1β ELISA kits were purchased from R&D Systems (Minneapolis, MN). Tauroursodeoxycholic acid (TUDC) was from Selleckchem (Houston, TX). Mouse IL-1β pro-form ELISA kit was from eBioScience (San Diego, California). Recombinant mouse IL-1β was from BioLegend (San Diego, CA). Cell culture ingredients were obtained from Invitrogen (San Diego, CA). All other reagents came from Sigma Chemical Co. (St. Louis, MO).