Growth curves were measured using a BioTek PowerWaveX2 microplate spectrophotometer. H. volcanii H53 (parent) and ΔartA, ΔhvpssA, and ΔhvpssD mutant strains carrying the plasmid expressing the complementary gene (or pTA963 as control) were first incubated in 5-ml liquid cultures in CA medium supplemented with tryptophan with continuous shaking at 45°C until suitable OD600 values (0.2 to 0.5) were reached. Approximately 6 μl of each culture (adjusted to correct for OD600 differences) was then transferred into 194 μl of fresh CA medium supplemented with tryptophan (50 μg ml−1 final concentration) and grown to stationary phase, with OD600 recordings taken every 30 min.
Powerwavex2
Manufactured by Agilent Technologies
Sourced in United States
The PowerWaveX2 is a high-performance lab equipment designed for precise waveform analysis. It offers advanced digital signal processing capabilities to capture and analyze complex waveforms with high accuracy and speed.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using powerwavex2
1
Growth Curves of H. volcanii Mutants
2
Growth Curve Analysis of H. volcanii
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Growth curves were generated using a Biotek PowerWaveX2 microplate spectrophotometer. H. volcanii strains were first incubated in 5-ml liquid cultures in CA medium supplemented with tryptophan and uracil with continuous shaking at 45°C, until suitable OD600 values (0.2–0.5) were reached. Approximately 2 μl of each culture (adjusted for OD600 differences) was then transferred into 198 μl of fresh CA medium supplemented with tryptophan and grown to stationary phase, with OD600 recordings taken every 30 min.
3
Liquid Hv-Cab Growth Curve Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid Hv-Cab starting cultures (5 mL) were inoculated from single colonies and grown at 45°C under shaking (250 rpm). After reaching an OD600 of 0.3 to 0.5 (measured in the culture tube, path length approximately 1.5 cm), all cultures were diluted to an OD600 of 0.05 in 20 mL to obtain comparable conditions at the start of the growth curve. Subsequently, OD600 was monitored for 74 hours; these measurements were performed by pipetting 250 μL of culture into a 96-well plate and measuring the absorption with a Biotek PowerWaveX2 microplate spectrophotometer. When cultures reached an OD above 0.75, samples were diluted 1:5 before measurement.
4
Quantitative Turbidimetric IgG Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantitative turbidimetric assay was used for the measurement of IgG in test samples [25 ]. The anti-rabbit IgG antibodies formed insoluble complexes when mixed with samples containing purified rabbit IgG (Invitrogen, Life technologies, Carlsbad, CA). The scattering of light by the immune-complexes to determine the IgG concentration in the sample was quantified by comparing with a calibrator of known IgG concentration (5 mg/ml to 0.625 mg/ml). The 50μl volume of each dilution was dispensed in duplicate in 96 well flat bottom microtitre plate (Greiner bio-one, Cellstar) and 50μl of anti-rabbit IgG prepared in tris buffer was added to these dilutions. Absorbance of each well was measured at 540 nm by ELISA plate reader (Biotek instruments, powerwave X2, USA) and plotted between absorbance and IgG concentration of each dilution. The IgG standard curve was used to determine the concentration of IgG in test samples.
5
Cytotoxicity evaluation of ZnO nanostructures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, the cytotoxicity of ZnONFs, CHCZnO NPs, and HC was evaluated on African monkey kidney (Vero) cells grown in a specific medium as previously described [15 (link)]. The cells were seeded in a 96-well cell culture plate and allowed to grow until they reached approximately 70% confluency. ZnONFs, CHCZnO NPs, and HC were added to the cells at various concentrations ranging from 2000 mg/mL to 62.5 mg/mL @10 µL per 100 µL media and incubated for one day. The next day, a resazurin dye was added to all the wells, and the optical densities were measured using a Biotek Instruments Powerwave X2 after 4 h. The cytotoxicity was calculated based on the untreated cells and the background coloration of the media, and the inhibitory concentration (IC50) was calculated using a specific equation [16 ].
where Y is the response value along with maximum (Max) and minimal (Min) values, and X is the inhibitory concentration. nH is Hill coefficient.
where Y is the response value along with maximum (Max) and minimal (Min) values, and X is the inhibitory concentration. nH is Hill coefficient.
6
MTT Cytotoxicity Assay of Silk Fibers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3T3 fibroblast cell lines were cultured in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum and 1% penicillin-streptomycin. The cells were maintained at 37°C, 5% CO2, and 95% relative humidity in a CO2 incubator.
The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazoliumbromide] cytotoxicity assay was carried out on the control and treated silk fibers. Cell viability was measured by MTT assay.22 (link) The 3T3 fibroblasts were seeded at a density of 1×105 cells per well in a 24-well plate containing silk fibers. After 24 and 120 hours of incubation, the medium was removed and the cells were washed with phosphate-buffered saline. The wells were then replaced with MTT (stock concentration 5 mg/mL). The plate was then incubated for 4 hours at 37°C. After incubation, the formazan reaction product was dissolved in solubilizing buffer and absorbance was measured at 570 nm using a microtiter plate reader (Powerwave X2, BioTek Instruments, Winooski, VT, USA). The morphology of cells treated with the silk fibers was viewed under an inverted phase contrast optical microscope (Axiostar II, Carl Zeiss, Oberkochen, Germany).
The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazoliumbromide] cytotoxicity assay was carried out on the control and treated silk fibers. Cell viability was measured by MTT assay.22 (link) The 3T3 fibroblasts were seeded at a density of 1×105 cells per well in a 24-well plate containing silk fibers. After 24 and 120 hours of incubation, the medium was removed and the cells were washed with phosphate-buffered saline. The wells were then replaced with MTT (stock concentration 5 mg/mL). The plate was then incubated for 4 hours at 37°C. After incubation, the formazan reaction product was dissolved in solubilizing buffer and absorbance was measured at 570 nm using a microtiter plate reader (Powerwave X2, BioTek Instruments, Winooski, VT, USA). The morphology of cells treated with the silk fibers was viewed under an inverted phase contrast optical microscope (Axiostar II, Carl Zeiss, Oberkochen, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!