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The L21415 is a laboratory instrument designed for DNA/RNA extraction and purification. It utilizes magnetic bead-based technology to isolate and purify nucleic acids from a variety of sample types. The core function of this product is to provide reliable and efficient nucleic acid extraction for downstream applications such as PCR, sequencing, and other molecular biology techniques.

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Lab products found in correlation

Hoechst, by Thermo Fisher Scientific (4 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (4 mentions) Lkgm002, by Cosmo Bio (3 mentions) Sc 13083, by Santa Cruz Biotechnology (1 mentions)

4 protocols using l21415

1

Immunostaining of Stomach Tissues

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Stomachs were prepared, stained and imaged using methods modified from Ramsey et al.42 (link) Briefly, paraffin-embedded specimens were cut into 5 μm sections, deparaffinised and rehydrated. After antigen retrieval with 10 mM sodium citrate (pH 6.0), sections were washed with PBS and blocked in 1% BSA and 0.3% Triton X-100 in PBS followed by overnight incubation with primary antibodies. The primary antibodies used for immunostaining were rat anti-CD44v (1:10 000 from Cosmo Bio, LKGM002) and goat anti-GIF (1:10,000, gift from Dr David Alpers, Washington University in St. Louis). After washing, sections were incubated with secondary antibodies and GS-II lectin (1:500, L21415, ThermoFisher). Sections were washed, stained with Hoechst (62249, ThermoFisher) 1:20 000 in PBS and mounted in ProLong Gold Antifade mountant (P36934, ThermoFisher).
Bockerstett K.A., Lewis S.A., Wolf K.J., Noto C.N., Jackson N.M., Ford E.L., Ahn T.H, & DiPaolo R.J. (2019). Single-cell transcriptional analyses of spasmolytic polypeptide-expressing metaplasia arising from acute drug injury and chronic inflammation in the stomach. Gut, 69(6), 1027-1038.
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2

Immunostaining of Gastric Tissues

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Stomachs were prepared, stained, and imaged using methods modified from Ramsey et al.57 (link) The primary antibodies used for immunostaining were goat anti–vascular endothelial growth factor B (1:100, sc-13083; Santa Cruz Biotechnology, Dallas, TX), anti-CD44v9 (1:10,000, LKGM002; Cosmo Bio, Carlsbad, CA), and goat anti-GIF (1:10,000; a gift from David Alpers, Washington University in St. Louis). After washing, sections were incubated with secondary antibodies and GS-II lectin (1:500, L21415; ThermoFisher, Waltham, MA). Sections were washed, stained with Hoechst (62249; ThermoFisher) 1:20,000 in PBS, and mounted in ProLong Gold Antifade mountant (P36934; ThermoFisher).
Bockerstett K.A., Petersen C.P., Noto C.N., Kuehm L.M., Wong C.F., Ford E.L., Teague R.M., Mills J.C., Goldenring J.R, & DiPaolo R.J. (2020). Interleukin 27 Protects From Gastric Atrophy and Metaplasia During Chronic Autoimmune Gastritis. Cellular and Molecular Gastroenterology and Hepatology, 10(3), 561-579.
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3

Stomach Tissue Immunostaining Protocol

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Stomachs were prepared, stained and imaged using methods modified from Ramsey et al.42 (link) Briefly, paraffin-embedded specimens were cut into 5 µm sections, deparaffinised and rehydrated. After antigen retrieval with 10 mM sodium citrate (pH 6.0), sections were washed with PBS and blocked in 1% BSA and 0.3% Triton X-100 in PBS followed by overnight incubation with primary antibodies. The primary antibodies used for immunostaining were rat anti-CD44v (1:10 000 from Cosmo Bio, LKGM002) and goat anti-GIF (1:10,000, gift from Dr David Alpers, Washington University in St. Louis). After washing, sections were incubated with secondary antibodies and GS-II lectin (1:500, L21415, ThermoFisher). Sections were washed, stained with Hoechst (62249, ThermoFisher) 1:20 000 in PBS and mounted in ProLong Gold Antifade mountant (P36934, ThermoFisher).
Bockerstett K.A., Lewis S.A., Wolf K.J., Noto C.N., Jackson N.M., Ford E.L., Ahn T.H, & DiPaolo R.J. (2019). Single-cell transcriptional analyses of spasmolytic polypeptide-expressing metaplasia arising from acute drug injury and chronic inflammation in the stomach. Gut, 69(6), 1027-1038.
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4

Immunostaining of Stomach Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stomachs were prepared, stained, and imaged using methods modified from Ramsey et. al.37 (link) Briefly, paraffin-embedded specimens were cut into 5 μm sections, deparaffinized and rehydrated. After antigen retrieval with 10 mM sodium citrate (pH 6.0), sections were washed with phosphate-buffered saline (PBS) and blocked in 1% bovine serum albumin and 0.3% Triton X-100 in PBS followed by overnight incubation with primary antibodies. The primary antibodies used for immunostaining were previously described rabbit anti-GKN3 serum (1:400),28 (link) rabbit anti-TFF2 (1:100, ab49536, Abcam), and goat anti-GIF (1:10,000, gift from David Alpers, Washington University in St. Louis). After washing, sections were incubated with secondary antibodies and GS-II lectin (1:500, L21415, ThermoFisher). Sections were washed, stained with Hoechst (62249, ThermoFisher) 1:20,000 in PBS and mounted in ProLong Gold Antifade mountant (P36934, ThermoFisher).
Bockerstett K.A., Lewis S.A., Noto C.N., Ford E.L., Saenz J.B., Jackson N.M., Ahn T.H., Mills J.C, & DiPaolo R.J. (2020). Single Cell Transcriptional Analyses Identify Lineage-Specific Epithelial Responses to Inflammation and Metaplastic Development in the Gastric Corpus. Gastroenterology, 159(6), 2116-2129.e4.
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