Cryptotanshinone of 99.26% purity (Selleckchem, Poznań, Poland), ≥99.9% methanol for HPLC (Chempur, Piekary Śląskie, Poland), 96% ethanol (Stanlab Sp. J., Lublin, Poland), ≥99.5% isopropanol (Fluka: BioChemika, Buchs, Switzerland), sodium hydroxide (Stanlab Sp. J.), 2-amino-2-methyl-1,3-propanediol (AMPD, Sigma-Aldrich, Poznań, Poland), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS, Sigma-Aldrich), 2,2′,2″-nitrilotriethanol (TEOA, Sigma-Aldrich), tris(2-propanol)amine (TIPA, Sigma-Aldrich), 96% sulfuric acid (VI) (Stanlab Sp. J., Lublin, Poland), 35–38% hydrochloric acid (HCl, POCH BASIC, Gliwice, Poland), acetonitrile for HPLC (Sigma-Aldrich), formic acid for HPLC (Sigma-Aldrich), and deionized water were used in the experimental procedures.
2 amino 2 hydroxymethyl 1 3 propanediol tris
Manufactured by Merck Group
Sourced in Poland
2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) is a chemical compound commonly used as a buffer in biochemical and molecular biology applications. It serves as a pH-regulating agent, helping to maintain a stable pH environment in various experimental setups.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by EKF Diagnostics (1 mentions)
Cryptotanshinone, by Selleck Chemicals (1 mentions)
Ethanol, by EKF Diagnostics (1 mentions)
Acetonitrile for hplc, by Merck Group (1 mentions)
2 amino 2 methyl 1 3 propanediol ampd, by Merck Group (1 mentions)
Formic acid for hplc, by Merck Group (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
H2so4, by Avantor (1 mentions)
Alkaline phosphatase, by US Biological (1 mentions)
Nuclease p1, by US Biological (1 mentions)
Forskolin, by Bio-Techne (1 mentions)
Ethylenediaminetetraacetic acid, by Thermo Fisher Scientific (1 mentions)
Adenosine monophosphate, by Merck Group (1 mentions)
Isoproterenol, by Merck Group (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid, by Thermo Fisher Scientific (1 mentions)
Ethylene glycol bis 2 aminoethylether n n n n tetraacetic acid, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate, by Bio-Techne (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
6 protocols using 2 amino 2 hydroxymethyl 1 3 propanediol tris
1
Cryptotanshinone Extraction and Purification
2
Nanomaterial Synthesis Protocol
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HNO3 (68%, BDH), ethanol (200
proof, Pharmco), H2O2 (30%, Fisher), H2SO4 (95.0%, J.T.Baker), tetrahydrofuran (THF, >99.5%,
Supelco), ethyl acetate (99.9%, Fisher), muscovite mica (Ruby, ASTM
V-1/V-2, S&J Trading), silver pellets (99.999%, Alfa Aesar), aluminum
pellets (99.999%, Kurt J. Lesker), DGEBA (Hexion Inc. EPON Resin 825),
EPON Resin 1004 F (Hexion Inc.), and 2-amino-2-(hydroxymethyl)-1,3-propanediol
(Tris, >99.9%, Sigma-Aldrich) were purchased and used as received.
Deionized (DI) water (>18.2 MΩ cm resistivity) was obtained
from an EMD Milli-Q Integral Water Purification System.
proof, Pharmco), H2O2 (30%, Fisher), H2SO4 (95.0%, J.T.Baker), tetrahydrofuran (THF, >99.5%,
Supelco), ethyl acetate (99.9%, Fisher), muscovite mica (Ruby, ASTM
V-1/V-2, S&J Trading), silver pellets (99.999%, Alfa Aesar), aluminum
pellets (99.999%, Kurt J. Lesker), DGEBA (Hexion Inc. EPON Resin 825),
EPON Resin 1004 F (Hexion Inc.), and 2-amino-2-(hydroxymethyl)-1,3-propanediol
(Tris, >99.9%, Sigma-Aldrich) were purchased and used as received.
Deionized (DI) water (>18.2 MΩ cm resistivity) was obtained
from an EMD Milli-Q Integral Water Purification System.
3
Calf Thymus DNA Extraction and Analysis
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Calf thymus genomic DNA was purchased from Sigma–Aldrich (St Louis, MO). The DNA was diluted in 10-mmol/l phosphate buffer, pH 7.4 at room temperature and aliquoted into 250-µl Eppendorf tubes containing 60 μg DNA each, and irradiated subsequently.
2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) (99.9% purity) was purchased from Sigma-Aldrich at a concentration of 30 mmol/l. Nuclease P1, snake venom phosphodiesterase, and alkaline phosphatase were purchased from United States Biological (Swampscott, MA), Sigma Chemical Co. (St Louis, MO) and Roche Applied Science (Indianapolis, IN), respectively. Water and acetonitrile for LC–MS/MS were purchased from Sigma Chemical Co. (St Louis, MO).
2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) (99.9% purity) was purchased from Sigma-Aldrich at a concentration of 30 mmol/l. Nuclease P1, snake venom phosphodiesterase, and alkaline phosphatase were purchased from United States Biological (Swampscott, MA), Sigma Chemical Co. (St Louis, MO) and Roche Applied Science (Indianapolis, IN), respectively. Water and acetonitrile for LC–MS/MS were purchased from Sigma Chemical Co. (St Louis, MO).
4
Evaluation of Forskolin and PMA Signaling
5
Comprehensive Onion and Egg Analysis
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Sodium dodecyl sulphate (SDS) was purchased from Beijing Biodee Biotechnology Co., Ltd. (Beijing, China). Hydrochloric acid (HCl) and trichloroacetic acid (TCA) were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China). The BCA protein assay kit was purchased from Thermo Fisher Technology Co., Ltd. (Shanghai, China). 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris) and 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) were obtained from Sigma-Aldrich Co., LLC. (Shanghai, China). Thiobarbituric acid (TBA) was bought from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). 1,1,3,3-tetraethoxypropane (MDA) was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). n-Alkane (C7–C40, purity ≥ 97%) and 2-methyl-3-heptanone with a purity of 99% were purchased from o2si Smart Solutions (Shanghai, China) and Dr. Ehrenstorfer (Beijing, China), respectively. Onion and egg were obtained from the local market (Beijing, China), and table salt was obtained from China Salt Industry Co., Ltd. (Shanghai, China).
6
Purification and Characterization of Cod Trypsin
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Dimethyl sulfoxide (DMSO), Succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide (Suc-AAPF-pNA), Benzoyl-L-Arg-p-nitroanilide (BAPNA), EDTA, Bis-Tris, glycine, sodium dodecyl sulphate (SDS), dithiothreitol (DTT), benzamidine, -mercaptoethanol, phenyl methyl sulphonyl fluoride (PMSF), soybean trypsin inhibitor, tosyl-lysyl-chloromethyl ketone (TLCK), Bowman-Birk trypsin inhibitor, 2-Amino-2-(hydroxymethyl)-1,3propanediol (TRIS), DEAE-Sepharose Fast Flow and Benzyloxycarbonyl-Gly-Pro-Arg pnitroanilide acetate salt (CBZ-GPR-pNA) were from Sigma Aldrich, St. Louis, MO, USA.
Calcium chloride dihydrate, sodium chloride, sodium hydroxide, methanol, glycerol, acetic acid and hydrochloric acid were from Merck, Darmstadt. Succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide (Suc-AAPA-pNA), Succinyl-L-Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide (Suc-AAPL-pNA) and Benzyloxycarbonyl-L-Phe-L-Arg-p-nitroanilide (CBZ-FR-pNA) were from Bachem AG, Bubendorf, Switzerland. Bio-Rad protein assay was from Bio-Rad laboratories, Hercules, California, USA. PageRuler Prestained Protein Ladder SDS from Fermentas (now Thermo Fisher Scientific, Rockford, IL). NucleoSpin Plasmid kit from Macherey-Nagel (US).
benzamidine purified cod trypsin was purified as described before [4] and obtained from Zymetech (Reykjavik, Iceland).
Calcium chloride dihydrate, sodium chloride, sodium hydroxide, methanol, glycerol, acetic acid and hydrochloric acid were from Merck, Darmstadt. Succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide (Suc-AAPA-pNA), Succinyl-L-Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide (Suc-AAPL-pNA) and Benzyloxycarbonyl-L-Phe-L-Arg-p-nitroanilide (CBZ-FR-pNA) were from Bachem AG, Bubendorf, Switzerland. Bio-Rad protein assay was from Bio-Rad laboratories, Hercules, California, USA. PageRuler Prestained Protein Ladder SDS from Fermentas (now Thermo Fisher Scientific, Rockford, IL). NucleoSpin Plasmid kit from Macherey-Nagel (US).
benzamidine purified cod trypsin was purified as described before [4] and obtained from Zymetech (Reykjavik, Iceland).
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