Log In Sign up
The largest database of trusted experimental protocols

Anti g9a

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Anti-G9a is a highly specific antibody that recognizes the G9a protein. G9a is a histone methyltransferase enzyme that catalyzes the mono- and di-methylation of histone H3 at lysine 9 (H3K9me1 and H3K9me2), playing a crucial role in epigenetic regulation.

Automatically generated - may contain errors

Lab products found in correlation

Anti h3k9me2, by Cell Signaling Technology (5 mentions) Anti h3k9me1, by Cell Signaling Technology (4 mentions) Ab40278, by Abcam (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Anti β actin, by Merck Group (2 mentions) Anti phospho β catenin ser33 37 thr41, by Cell Signaling Technology (2 mentions) Anti nrf2, by Cell Signaling Technology (2 mentions) Filipin, by Merck Group (2 mentions) Anti β catenin, by Cell Signaling Technology (2 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (2 mentions) Anti sirt6, by Cell Signaling Technology (2 mentions) Alexa488 conjugated anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Hrp conjugated anti β actin antibody, by Merck Group (2 mentions) Hrp conjugated anti rabbit total igg, by Jackson ImmunoResearch (2 mentions) Pe conjugated f4 80, by Cytek Biosciences (2 mentions) Anti ser9 phospho gsk 3β, by Cell Signaling Technology (2 mentions) Anti ho 1, by Cell Signaling Technology (2 mentions) Anti trxr1, by Cell Signaling Technology (2 mentions) Anti lrp2 antibody, by Santa Cruz Biotechnology (2 mentions) Anti srebp2 antibody, by Abcam (2 mentions)

Close spelling variants of this product

Anti-G9a (3306 (4 citations) Anti-G9a antibody (4 citations) Anti-G9a (clone C6H3) (2 citations) Rabbit anti-G9a (clonal C6H3) (2 citations)

16 protocols using anti g9a

1

Western Blot Analysis of RUNX3, G9a, and Methylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously described [7 (link)]. Antibodies specific to human proteins were anti-RUNX3 (ab40278, Abcam), anti-G9a (#3306, Cell Signaling Technology), and β-actin (SC-47778, Santa Cruz), pan-methyl lysine antibody (ab23366, Abcam).
Lee S.H., Hyeon D.Y., Yoon S.H., Jeong J.H., Han S.M., Jang J.W., Nguyen M.P., Chi X.Z., An S., Hyun K.G., Jung H.J., Song J.J., Bae S.C., Kim W.H., Hwang D, & Lee Y.M. (2020). RUNX3 methylation drives hypoxia-induced cell proliferation and antiapoptosis in early tumorigenesis. Cell Death and Differentiation, 28(4), 1251-1269.
+ Open protocol
+ Expand
2

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates for western blotting were harvested in Laemmli lysis buffer (62.5 mM Tris-HCl, pH 6.8, 20% glycerol, 2% SDS, 2 mM DTT) with proteinase inhibitor freshly added. The following primary antibodies were used for immunoblotting: anti-BRD4 (Bethyl, A301-985-50, dilution 1:5,000), anti-Myogenin (Santa Cruz Biotechnology, sc-576, dilution 1; 500), anti-Troponin-T (Sigma, T6277, dilution 1:2000), anti-β-actin (Sigma, A1978, dilution 1: 10,000), anti-G9a (Cell Signaling, 3306S, dilution 1: 300), anti-H3K9ac (Abcam, ab4441, dilution 1:1,000), and normal rabbit IgG (Santa Cruz Biotech, sc-2027).
Yang N., Das D., Shankar S.R., Goy P.A., Guccione E, & Taneja R. (2022). An interplay between BRD4 and G9a regulates skeletal myogenesis. Frontiers in Cell and Developmental Biology, 10, 978931.
+ Open protocol
+ Expand
3

Immunofluorescence Analysis of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
HRP-conjugated anti-β-ACTIN antibody, Filipin and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich/ Merck Millipore. Alexa488-conjugated anti-rabbit IgG, HRP-conjugated anti-rabbit total IgG and light chain specific IgG antibodies were purchased from Jackson ImmunoResearch, USA; PE-conjugated F4/80 was procured from Tonbo Biosciences, USA. Alexa Fluor 660 conjugated CD68 was purchased from ThermoFischer Scientific. Anti-G9a, anti-SIRT6, anti-H3K9me1, anti-H3K9me2, anti-H3K9Ac, anti-Ser33/37/Thr41 phospho-β-CATENIN, anti-Ser9 phospho-GSK-3β, anti-β-CATENIN, anti-NRF2, anti-HO1 and anti-TRXR1 antibodies were obtained from Cell Signaling Technology, USA. Anti-LRP2 antibody was purchased from Santa Cruz Biotechnology, USA; anti-SREBP2 antibody was procured from Abcam, USA; and anti-NQO1 antibody was purchased from Calbiochem, USA.
Prakhar P., Bhatt B., Lohia G.K., Shah A., Mukherjee T., Kolthur-Seetharam U., Sundaresan N.R., Rajmani R.S, & Balaji K.N. (2023). G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival. PLOS Pathogens, 19(10), e1011731.
+ Open protocol
+ Expand
4

Signaling Pathway Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with ice-cold phosphate-buffered saline (PBS) and then solubilized with protease inhibitor (Upstate Biotechnology, Temesula, CA, USA)-containing RIPA buffer. Lysates were immunoblotted with indicated antibodies, anti-G9a, anti-DUSP4, anti-p-AMPK, anti-AMPK, anti-S6K and anti-p-S6K (T389) (Cell Signaling Technology), anti-H3K9me1 (Millipore, MA, USA), anti-GLP, anti-H3K9me2, anti-histone 3, anti-LC3B (Abcam), anti-H3K9me3, anti-caspase 3, anti-PARP, anti-p62, anti-β-actin anti-GAPDH, anti-α-tubulin (GeneTex), anti-p-Akt, anti-Akt, anti-p-ERK and anti-ERK (Santa Cruz Biotechnology, Santa Cruz, CA).
Li K.C., Hua K.T., Lin Y.S., Su C.Y., Ko J.Y., Hsiao M., Kuo M.L, & Tan C.T. (2014). Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma. Molecular Cancer, 13, 172.
+ Open protocol
+ Expand
5

Analyzing RUNX3 and G9a in Gastric Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human gastric tissues specimens were provided from the Seoul National University Hospital. Proteins were extracted from human gastric tumor or normal tissues by tissue homogenization in RIPA buffer (Thermo Scientific). Tissue lysates were subjected to western blot analysis sing anti-RUNX3 (ab40278, Abcam), anti-G9a (#3306, Cell Signaling Technology), and β-actin (SC-47778, Santa Cruz) antibodies. This study was approved by the Institutional Review Board of Seoul National University Hospital (1706-105-860), and informed consent was obtained from each subject.
Lee S.H., Hyeon D.Y., Yoon S.H., Jeong J.H., Han S.M., Jang J.W., Nguyen M.P., Chi X.Z., An S., Hyun K.G., Jung H.J., Song J.J., Bae S.C., Kim W.H., Hwang D, & Lee Y.M. (2020). RUNX3 methylation drives hypoxia-induced cell proliferation and antiapoptosis in early tumorigenesis. Cell Death and Differentiation, 28(4), 1251-1269.
+ Open protocol
+ Expand
6

Chromatin Immunoprecipitation of Epigenetic Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assay was performed using a SimpleChIP Enzymatic Chromatin IP kit (Cell Signaling Technology) according to the manufacturer’s instructions. Each immunoprecipitation was performed using 1 μg of following antibodies: anti-H3K9me2 (#4658), anti-Myc (#9402), anti-Max (#4739), anti-G9a (#3306) (from Cell Signaling Technology), anti-TFIID (sc-421), anti-c-Ets1 (sc-55581), anti-pSTAT4 (sc-28296), anti-Foxp3 (sc-53876), anti-HNF1A (sc-393925), anti-HNF3a (sc-101058) (from Santa Cruz Biotechnology). Isotype normal IgG was used as a negative control. ChIP primers were designed to amplify the different sites in 15-PGDH promoter regions by real time PCR. The primers are as follows: P_set1 F1, 5’- GAG CAA GGA ACC TCT GTC CC -3’; R1, 5’- TGT CAT CAT CAA CAG GCG CT -3’; P_set2 F2, 5’- TGC TTA GCG GCT TAC CAA CA -3’; R2, 5’- GGG GAA ATG GGA GTT GAG CA -3’; P_set3 F3, 5’- CCT GGT GCT CAC CTG AGT ATT -3’; R3, 5’- ATG GCA ACA TGC TGG GAG AA -3’; P_set4 F4, 5’- CTG GAC AGT GGC AGT GGA AA -3’; R4, 5’- AGC AAG GAC TGA GGT CTA GAG AA -3’. BioRad SsoAdvanced™ Universal SYBR® Green Supermix was used to amplify the target DNA fragments on a Bio-Rad C1000 Thermal Cycler.
Zhang J., Chen W., Ma W., Song K., Lee S., Han C, & Wu T. (2022). Epigenetic Silencing of 15-Hydroxyprostaglandin Dehydrogenase by Histone Methyltransferase EHMT2/G9a in Cholangiocarcinoma. Molecular cancer research : MCR, 20(3), 350-360.
+ Open protocol
+ Expand
7

G9a Regulation of SNAIL Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
G9a-knockdown cells were selected by cell sorting for EGFP expression. These cells and BIX-01294-treated HCC cells were subjected to Western blot analysis using anti-G9a (Cell Signaling Technology, Danvers, MA), anti-tubulin (Oncogene Science, Cambridge, MA), anti-histone H3K9me2 and anti-histone H3 (Millipore, Billerica, MA) antibodies. Endogenous SNAIL was immunoprecipitated and subjected to Western blotting with rabbit anti-SNAIL (Cell Signaling Technology) and mouse anti-G9a (Abcam, Cambridge, MA) antibodies.
Yokoyama M., Chiba T., Zen Y., Oshima M., Kusakabe Y., Noguchi Y., Yuki K., Koide S., Tara S., Saraya A., Aoyama K., Mimura N., Miyagi S., Inoue M., Wakamatsu T., Saito T., Ogasawara S., Suzuki E., Ooka Y., Tawada A., Otsuka M., Miyazaki M., Yokosuka O, & Iwama A. (2017). Histone lysine methyltransferase G9a is a novel epigenetic target for the treatment of hepatocellular carcinoma. Oncotarget, 8(13), 21315-21326.
+ Open protocol
+ Expand
8

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed using the following primary antibodies as previously described,17 (link) anti-G9a, anti-phospho c-Jun N-terminal kinase (anti-p-JNK), anti-JNK anti-p-P38, anti-p-ERK anti-H3K9me2, anti-cleaved poly(ADP-ribose) polymerase 1 (PARP1), anti-cleaved caspase-8 and GAPDH (Cell Signaling Technology).
Lou H., Pan H., Huang Z., Wang Z, & Wang D. (2019). Inhibition of G9a promoted 5-fluorouracil (5-FU) induced gastric cancer cell apoptosis via ROS/JNK signaling pathway in vitro and in vivo. RSC Advances, 9(26), 14662-14669.
+ Open protocol
+ Expand
9

Antibody panel for chromatin research

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies are used for this study: anti-α-Tubulin (clone B-5-1-2, Sigma-Aldrich); anti-FLAG M2 antibody (F3165, Sigma-Aldrich); anti-V5 antibody (R960-25, Thermo Fisher which is same as #46-0705, Life technology); anti-G9a (#8620,(11)); anti-GLP (#0422,(11)); anti-SUV39H1 (clone D11B6, Cell signalling); anti-MCAF1/ATF7IP [48 (link)] and anti-ATF7IP (ab84497, Abcam); anti-MPP8 (16796-1-AP, Proteintech); anti-H3 (07-690, Millipore); anti-Pan-Kme2 (c4930/c5123 mix.(19)); anti-Pan-Kme3 (76118, Abcam); anti-SETDB1/ESET (cp10377, Cell Applications).
Tsusaka T., Kikuchi M., Shimazu T., Suzuki T., Sohtome Y., Akakabe M., Sodeoka M., Dohmae N., Umehara T, & Shinkai Y. (2018). Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8. Epigenetics & Chromatin, 11, 56.
+ Open protocol
+ Expand
10

Embryo Immunofluorescence Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryos were treated as previously described (Nichols and Smith, 2009 (link)). Primary antibodies used are as follows: anti-CDX2 (Biogenex, clone CDX2-88), anti-H3K9me2 (Abcam, UK, ab1220), anti-GFP (Nacalai tesque, Japan, GF090R), anti-G9a (Cell Signaling, MA, 68851T), anti-GLP (Research and Diagnostic Systems, MN, PP-B0422-00), anti-SOX2 (Abcam, UK, ab92494), anti-SOX17(Research and Diagnostic Systems, MN, AF1924). Mean nuclear intensities of IF and DAPI signal were quantified using mageJ and corrected for the staining background. As nuclear size is changing between stages all IF measurements were normalised to DAPI signal as a proxy for DNA content.
Zylicz J.J., Borensztein M., Wong F.C., Huang Y., Lee C., Dietmann S, & Surani M.A. (2018). G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst. eLife, 7, e33361.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!