Twenty-six cholecystolithiasis patients who received laparoscopic cholecystectomy in the First Affiliated Hospital of Harbin Medical University between February 2017 and March 2018 were enrolled. The study participants were randomly divided into experimental (Berberine; n = 11–14) and control (Placebo; n = 9–12) groups. The subjects of the experimental group were given 6 mL of polyethylene glycol (PEG) containing 1.5‰ berberine (polyethylene glycol berberine; brand name: Zhan Lian Ping, Heilongjiang Liaoyuan Science and Technology Co., Ltd., China) in the surface of gallbladder forssa and adjacent intestine, omentum majus and stomach before suturing the incision. The patients of the Placebo group received an equal volume of PEG (medical PEG, brand name: Xian Taihua Medical Co., Ltd., China) without berberine. Plasma from enrolled patients were collected prior to the surgery, as well as 12 and 24 h after administration of berberine. ELISA assays were used to measure plasma concentrations of TIMP-1 (BOSTER, Pleasanton, CA, USA), active MMP-3 (BOSTER) and MMP-8 (BOSTER).
Mmp 8
Manufactured by Boster Bio
Sourced in United States
MMP-8 is a lab equipment product used for the detection and measurement of matrix metalloproteinase-8 (MMP-8), also known as neutrophil collagenase. MMP-8 is an enzyme involved in the breakdown of extracellular matrix components and plays a role in various biological processes.
Automatically generated - may contain errors
2 protocols using mmp 8
1
Berberine Effects on Cholecystolithiasis Surgery
2
Protein Expression Analysis in Rat Kidney
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A frozen kidney tissue of rats in each group was taken to extract proteins and estimate the protein by bicinchoninic acid assay (BCA) colorimetric method. The protein was denatured and separated by SDS-PAGE electrophoretic technique and transferred to a polyvinylidene difluoride (PVDF) membrane. After formulating with 5% bovine serum albumin (BSA) for 2 h, the blocked membranes were incubated with ERK1/2 (cell signaling), TGF-β1, MMP-2, MMP-7, MMP-8, MMP-11, MMP-14, TIMP1, and TIMP2 (Boster Biological Technology, Ltd., Wuhan, PR China) antibody, and then washed with tris buffered saline with Tween (TBST), and detected with a secondary antibodies (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD), and finally these membranes were subjected to chemiluminescence detection assay.
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